Effect of Migration and Capacitation on the Nuclear Stability of Human Sperm

Huret, JL; Courtot, Anne-Marie

doi:10.3109/01485018408987513

Cited by 8 publications

(8 citation statements)

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“…We observed a significant increase in Feulgen-DNA content as well as in mean nuclear surface area both in B2 and in FF. Such results do not confirm earlier results concerning the effect of migration and capacitation on the nuclear stability of human sperm (Huret and Courtot, 1984). However, that study used a nuclear chromatin decondensation ability test that evaluated the induced swelling of the spermatozoa heads.…”

Section: Discussioncontrasting

confidence: 81%

“…Many studies have highlighted the condensationldecondensation process that affects mammalian spermatozoa chromatin either during the spermiogenesislsper-bility as suggested by a nuclear decondensation ability test (Huret and Courtot, 1984). This paper reports on two experiments designed to study the effects on human sperm chromatin of in vitro capacitation without decondensing agents.…”

Section: Introductionmentioning

confidence: 99%

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Does in vitro capacitation alter chromatin stability of ejaculated human spermatozoa? cytochemical studies

Royère

Hamamah

Nicolle

et al. 1991

Molecular Reproduction Devel

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In vitro capacitation of human spermatozoa is commonly evaluated by the progressive motility percent. However its effects on sperm chromatin have hardly been studied. Our aim was to determine the extent to which in vitro capacitation with two treatments (B2 or human follicular fluid) alters the chromatin of human spermatozoa, by using two analytical methods, acridine orange staining and Feulgen-DNA cytophotometric measures. Ejaculates were obtained from 23 men participating in our in vitro fertilization program, and several measurements were made on the same ejaculate for each subject. No alteration was observed for the percent of native DNA after capacitation in B2, but spermatozoa incubation during the same time in human follicular fluid was followed by a significant decrease of the percent of native DNA (P less than 0.01). Feulgen-DNA content significantly increased after capacitation in either B2 or follicular fluid (P less than 0.05, P less than 0.001 respectively), and so did sperm nuclear surface area (P less than 0.001). In this study we observed a negative correlation between Feulgen-DNA content and fertilization rate (P less than 0.02). Moreover, the greater effects on Feulgen-DNA content were observed in men with abnormal sperm, whose spontaneous percent of native DNA was lower (P less than 0.05) and Feulgen-DNA content higher (P less than 0.05) than in men with normal sperm. These results indicate that capacitation in B2 as well as in human follicular fluid may alter the chromatin stability of human spermatozoa. Such results suggest a partial decondensation state of human spermatozoa during in vitro capacitation. However, beyond some level of decondensation, the fertilizing ability could be altered.

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Section: Discussioncontrasting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Does in vitro capacitation alter chromatin stability of ejaculated human spermatozoa? cytochemical studies

Royère

Hamamah

Nicolle

et al. 1991

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…On the other hand, it has been found that migration also selects a population with a better-stabilized nucleus [21]. It is therefore probable that a deficient spermiation and/or an incomplete maturation could allow an hypostabilization of the chromatin [21]. This hypothesis seems to be confirmed by the results obtained on a population of teratozoosperm [6].…”

Section: Optimal Stability Of the Nucleus And In Vivo Decondensationsupporting

confidence: 78%

“…It is known that in vitro swim-up migration selects sperm for good morphology and motility [36] and then eliminates most of the sperm having had a deficient spermiogenesis or an incomplete maturation in the epididymis. On the other hand, it has been found that migration also selects a population with a better-stabilized nucleus [21]. It is therefore probable that a deficient spermiation and/or an incomplete maturation could allow an hypostabilization of the chromatin [21].…”

Section: Optimal Stability Of the Nucleus And In Vivo Decondensationmentioning

confidence: 99%