In laboratory experiments samples of natural or chloroform‐fumigated soils were inoculated with an Alcaligenes xylosoxidans subsp. denitrificans which is able to use 2,4‐dichlorophenoxyacetic acid (2,4‐D) as a sole carbon source. Biotic factors affecting survival and activity of the inoculant were determined. In natural soil the numbers and activity of Alc. xylosoxidans declines in few days. The strain proliferated only when it was inoculated immediately after soil fumigation. Its activity 15 d after inoculat…
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