Effect of Microalgal Extracts of Tetraselmis suecica against UVB-Induced Photoaging in Human Skin Fibroblasts

Abstract

Exposure of cells to ultraviolet B (UVB) radiation can induce production of free radicals and reactive oxygen species (ROS), which damage cellular components. In addition, these agents can stimulate the expression of matrix metalloproteinase (MMP) and decrease collagen synthesis in human skin cells. In this study, we examined the anti-photoaging effects of extracts of Tetraselmis suecica (W-TS). W-TS showed the strongest scavenging activity against 2,2-difenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals, followed by superoxide anions from the xanthine/xanthine oxidase system. We observed that the levels of both intracellular ROS and lipid peroxidation significantly increased in UVB-irradiated human skin fibroblast cells. Furthermore, the activities of enzymatic antioxidants (e.g., superoxide dismutase) and the levels of non-enzymatic antioxidants (e.g., glutathione) significantly decreased in cells. However, W-TS pretreatment, at the maximum tested concentration, significantly decreased intracellular ROS and malondialdehyde (MDA) levels, and increased superoxide dismutase and glutathione levels in the cells. At this same concentration, W-TS did not show cytotoxicity. Type 1 procollagen and MMP-1 released were quantified using RT-PCR techniques. The results showed that W-TS protected type 1 procollagen against UVBinduced depletion in fibroblast cells in a dose-dependent manner via inhibition of UVB-induced MMP-1. Taken together, the results of the study suggest that W-TS effectively inhibits UVB-induced photoaging in skin fibroblasts by its strong anti-oxidant ability.

The green microalga Tetraselmis suecica reduces oxidative stress and induces repairing mechanisms in human cells

“…have potential cosmetic and pharmaceutical applications for the human hair growth and pigmentation of the skin, and also for stimulating the increased production of skin structural proteins such as filaggrin and involucrin involved in dermal diseases as Psoriasis8. In a previous study, an ethanol/water extract of Tetraselmis suecica showed a strong scavenging activity against 2,2-difenyl-1-picrylhydrazyl (DPPH) and peroxyl radicals rather than against the superoxide anions from the xanthine/xanthine oxidase system9. Here we show that a similar type of extract from T. suecica also stimulates a strong response to cell damage and activates a repairing mechanism in human epidermal cells.…”

Protective properties of geniposide against UV-B-induced photooxidative stress in human dermal fibroblasts

“…Chronic exposure to UV-B radiation typically brings about the enhanced generation of reactive oxygen species (ROS) in the skin, which leads to oxidative stress and photodamages to macromolecules, such as proteins and nucleic acids (Jo et al. 2012 ). It also causes adverse changes in the extracellular matrix that lead to skin photoaging due to the enhanced production of matrix metalloproteinases (MMPs), including MMP-1, MMP-2 and MMP-9, which is possibly mediated by augmented ROS production (Ho et al.…”

Neferine prevents ultraviolet radiation‑induced skin photoaging

“…Glutathione (GSH) is the primary intracellular non-enzymatic antioxidant defense against ROS and serves a central role in the reduction of oxidative stress. GPx uses GSH as a hydrogen donor to reduce H 2 O 2 and hydroperoxides to H 2 O and O 2 (35). The imbalance between antioxidants and free radicals, which is either caused by a decrease in antioxidants or an increase in ROS, leads to cell damage and cell death (36).…”