Effect of microalgae Messastrum gracile and Chlorella vulgaris on the in vitro propagation of orchid Cattleya labiata

Corbellini, Joana Rosar; Ribas, Luciana Lopes Fortes; Maia, Fabiano Rodrigo da; Corrêa, Diego de Oliveira; Noseda, Miguel D.; Suzuki, Rogério Mamoru; Amano, Érika

doi:10.1007/s10811-020-02251-9

Cited by 13 publications

(17 citation statements)

References 33 publications

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“…Data in the literature corroborated findings in the current study, since culture medium supplementation with 1 g L -1 of activated charcoal reduced oxidation and contamination in comparison to the treatment without it, during the micropropagation of Cattleya walkeriana (SOUZA et al, 2007) and Eugenia pyriformis (ASSIS et al, 2018). Futhermore, the germination and elongation phases, in vitro, also demand the control of oxidation caused by phenolic exudation in the region where explants are cut; oxidation was also responsible for explant darkening due to the activity of enzymes such as peroxidase and polyphenol oxidase (WILLADINO et al, 2013;CORBELLINI et al, 2020).…”

Section: Activated Charcoal In the In Vitro Germinationmentioning

confidence: 99%

“…With respect to the negative effects of activated charcoal supplementation, it is possible highlighting the adsorption of growth regulators (auxins and cytokinins), vitamins and of other organic substances important for explant in vitro development and growth. Activated charcoal also naturally releases substances that are found on, or adsorbed by, it (ABIRI et al, 2020;CORBELLINI et al, 2020).…”

Section: Activated Charcoal In the In Vitro Elongationmentioning

confidence: 99%

“…The definition of an appropriate culture medium is a limiting factor for orchid micropropagation (KIM et al, 2019); therefore, it is essential for studies carried out in each in vitro cultivation phase of C. crispata. Activated charcoal is among the components that can be added to culture media in order to act as antioxidant and to promote the adsorption of auxins and cytokinins, plant exudates and toxic metabolites (ANUCHAI et al, 2017;CORBELLINI et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Souza

Fernandes

Molinari

et al. 2021

NAT

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Micropropagation is an alternative for the genetic conservation and propagation of endemic species from “Campo Rupestre Ferruginoso”, such as the orchid Cattleya crispata. The aim of the present study is to assess the influence of activated charcoal on the in vitro germination, multiplication and elongation phases of C. crispata. Seeds extracted from mature capsules were used for inoculation in the culture medium that was adopted to assess the effect of supplementation, or not, with activated charcoal. Data about germination speed, seedling number, length, vigor, oxidation and contamination (bacterial and/or fungal) were assessed through these phases. Based on the results obtained, the use of activated charcoal was efficient in the in vitro germination and multiplication phases of C. crispata, providing greater speed and percentage of germination, less contamination and oxidation of the tissues, greater number, length and vigor of shoots, being effective for the genetic conservation and production of seedlings of the species. Culture medium without the supplementation of activated charcoal provided the best results for the in vitro elongation, with greater length, vigor and less oxidation of shoots.

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Section: Activated Charcoal In the In Vitro Germinationmentioning

confidence: 99%

Section: Activated Charcoal In the In Vitro Elongationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Souza

Fernandes

Molinari

et al. 2021

NAT

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“…Other additives that can be used on in vitro culture are polyamines (PAs), which are organic polycationic compounds, consisting of two or more amino groups, used to improve the growth and development of plants, due to their effects on cell division and differentiation (Abbasi et The regenerated PLBs can be grown in culture medium, without PGRs and with the addition of activated charcoal to stimulate elongation and root development. This strategy has been effective for some orchids, such as Hadrolaelia grandis (Vudala et al 2019)d labiata (Corbellini et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Effect of microalga Desmodesmus subspicatus, polyamines and plant growth regulators on the in vitro propagation of Cattleya warneri

Navarro

Corrêa

Behling

et al. 2022

Preprint

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Cattleya warneri, an orchid with high ornamental potential, suffers indiscriminate harvest and is classified as a vulnerable species, requiring propagation studies. The aim of this study was to evaluate the effect of biomass or aqueous extract of Desmodesmus subspicatus microalga, polyamines (PAs) and plant growth regulators (PGRs): 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA) on the in vitro propagation of C. warneri using the thin cell layer (TCL) technique. Entire protocorms and transversal sections (tTCLs) or longitudinal sections (lTCLs) were grown in MS culture medium, with the concentration of macronutrients reduced by half (MSM/2) containing D. subspicatus biomass or extract Pas, and BA and/or NAA. The results indicated that the apical tTCLs were the best explants and the entire protocorms formed seedlings. The regeneration of protocorm-like bodies (PLBs) was more efficient with the addition of 1.5 g L− 1 of microalgae biomass (92% and 4.7 PLBs) or 1.0 g L− 1 of extract (100% and 3.3 PLBs) and 8 µM BA (89% and 5.2 PLBs). The biomass (1.0 g L− 1) or extract (2.0 g L− 1) also enabled PLBs regeneration from entire protocorms (70% and 4.0 PLBs, 50% and 3.2 PLBs, respectively). The plant acclimatization was recommended using coconut fiber and vermiculite (1:1, v/v) as substrate (survival rate 84%% and greater fresh mass 0.895 g). In conclusion, a rapid and efficient protocol for the mass propagation of C. warneri was achieved using the TCL technique. In addition, MSM/2 medium supplementation with biomass and extract of D. subspicatus is an effective alternative to replace conventional PGRs.

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“…Mazepa et al (2021) avaliaram a aplicação da biomassa e do extrato aquoso da microalga Desmodesmus subspicatus na germinação e crescimento de plantas de alface, identificando a presença de glicosídeos e da citocinina natural zeatina, responsáveis pela atividade observada. Além da aplicação diretamente no campo, os extratos das microalgas D. subspicatus, Chlorella vulgaris e Messastrum gracile demonstraram potencial aplicação na propagação in vitro de oruídeas, ampliando as possibilidades de aplicação das microalgas na agricultura(CORBELLINI et al, 2020;NAVARRO et al, 2021).…”

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Produção de biomassa microalgal em escala: desafios e oportunidades

Santos¹,

Corrêa²,

Selesu³

et al. 2021

Microalgas: Potenciais Aplicações E Desafios

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Effect of microalgae Messastrum gracile and Chlorella vulgaris on the in vitro propagation of orchid Cattleya labiata

Cited by 13 publications

References 33 publications

ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

ACTIVATED CHARCOAL APPLICATION FOR THE MICROPROPAGATION OF Cattleya crispata (Thunb.) Van den Berg

Effect of microalga Desmodesmus subspicatus, polyamines and plant growth regulators on the in vitro propagation of Cattleya warneri

Produção de biomassa microalgal em escala: desafios e oportunidades

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