We examined the change in the content of pectic substances in tea leaves during the steaming of green tea. In four cultivars of tea leaves, water-soluble pectin (WSP) content increased with steaming time, whereas the dilute hydrochloric acid soluble pectin (HSP) content remained almost constant. A β-elimination reaction proceeds throughout the steaming process, so it was postulated that some water-insoluble forms of pectin in HSP convert to a water-soluble form via β-elimination degradation with increasing steaming time. When tea leaves were steamed, pectin fractions with a molecular weight of 1,100 × 10 3 were degraded from water-insoluble HSP and eluted as WSP.Additionally, as steaming time increased, the fraction of smaller molecular weight pectin increased.Keywords: pectic substances, green tea, steaming process
IntroductionIt has been elucidated that the bitter or astringent taste of catechins and the umami taste of amino acids such as theanine are important factors in the taste of green tea (Nakagawa et al., 1981).The taste of green tea changes depending not only on the conditions used to brew the tea, but also on how the tea leaves are processed.When raw tea leaves are processed differently, the tea products differ in quality. As an example, fukamushi tea steamed for a longer time tastes less astringent than asamushi tea steamed for a shorter time. The amounts of several components, such as catechins and amino acids, have typically been considered to be indices of the quality of the tea. However, the amounts of these components do not change during the course of tea processing (Takayanagi and Anan, 1986). In contrast, the amount of water-soluble pectin (WSP) increases during the steaming process with increased steaming time (Takayanagi et al., 1987). It was therefore assumed that the increase in WSP masked the astringent taste of catechins, as fukamushi tea tastes less astringent (Horie and Kohata, 1999 et al., 1960). In this paper, we examined the change in the content of pectic substances in tea leaves during the steaming process by varying cultivars, harvesting time, and maturity of the tea leaves. Additionally, we investigated the extent of b-elimination degradation and the molecular weight distribution of WSP during the steaming process. from the first crop season were also plucked by their degree of maturity: younger, optimum, and over-matured. On average, the interval between these three levels of maturity was 2 days. About 100 g of plucked tea leaves (raw leaves) were immediately frozen and the rest were steamed. About 100 g of tea leaves were steamed for 30, 60, 120, and 300 s for the 'Yabukita' cultivar, and for 60 and 300 s for the other three cultivars. The steamed leaves were then frozen. All frozen tea leaves were lyophilized and powdered using a cyclone sample mill. was stirred and heated in a boiling water bath for 10 min. After cooling to room temperature, the optical density was measured at 548 nm in a spectrophotometer.
Materials and Methods
Preparation of tea leaves
Preparation of al...