Effect of membrane hydrophilization on ultrafiltration performance for biomolecules separation

Susanto, Heru; Roihatin, Anis; Aryanti, Nita; Anggoro, Diky; Ulbricht, Mathias

doi:10.1016/j.msec.2012.04.036

Cited by 28 publications

(22 citation statements)

References 19 publications

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“…This, however, requires post‐functionalization chemistry which can be carried out in non‐solvents for the membrane. Such strategy has been successfully demonstrated on various commercial ultrafiltration membranes . For integral asymmetric PS‐ b ‐P4VP membranes it was demonstrated that a surface modification with dopamine leading to a polydopamine layer is possible .…”

Section: Discussionmentioning

confidence: 99%

Isoporous Block Copolymer Membranes

Abetz¹

2014

Macromol. Rapid Commun.

217

241

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The developments in membranes based on tailored block copolymers are reported with an emphasis on isoporous membranes. These membranes can be prepared in different geometries, namely flat sheets and hollow fibers. They display narrow pore size distributions due to their formation by self-assembly. The preparation of these membranes and possibilities to further functionalize such membranes will be discussed. Different ways to control the pore size will be addressed, and the potential of block copolymer blends to fabricate membranes with tailored pore sizes will be shown.

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Section: Discussionmentioning

confidence: 99%

Isoporous Block Copolymer Membranes

Abetz¹

2014

Macromol. Rapid Commun.

217

241

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“…Biomolecules separation is important in biology, medicine, and chemistry . Herein, pDNA a flexible biomolecule was used in this study.…”

Section: Resultsmentioning

confidence: 99%

Modification of microfiltration membranes by hydrogel impregnation for pDNA purification

Castilho

Correia

Amorim

et al. 2014

J of Applied Polymer Sci

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The huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane‐based technologies are the best match to assure simplicity, efficiency and easy scale‐up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR‐FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41610.

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“…[ 92 ] Susanto (2012) demonstrated reduced fouling when a hydrophilic monomer, poly(ethylene glycol) methacrylate, was drafted onto a commercial polyethersulfone (PES) UF membrane. [ 93 ] The modified membranes exhibited a flux drop of 20% compared to an 80% drop observed in unmodified membranes having higher hydrophobicity. The filtration performance of commonly used PES and polysulfone‐based membranes are prone to deterioration over time owing to repeated CIP procedures using strong alkalis.…”

Section: Advances In Membrane Technology For Downstream Applicationsmentioning

confidence: 99%

Intensified Downstream Processing of Monoclonal Antibodies Using Membrane Technology

Nadar

Shooter

Somasundaram

et al. 2020

Biotechnology Journal

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The need to intensify downstream processing of monoclonal antibodies to complement the advances in upstream productivity has led to increased attention toward implementing membrane technologies. With the industry moving toward continuous operations and single use processes, membrane technologies show promise in fulfilling the industry needs due to their operational flexibility and ease of implementation. Recently, the applicability of membrane‐based unit operations in integrating the downstream process has been explored. In this article, the major developments in the application of membrane‐based technologies in the bioprocessing of monoclonal antibodies are reviewed. The recent progress toward developing intensified end‐to‐end bioprocesses and the critical role membrane technology will play in achieving this goal are focused upon.

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Effect of membrane hydrophilization on ultrafiltration performance for biomolecules separation

Cited by 28 publications

References 19 publications

Isoporous Block Copolymer Membranes

Isoporous Block Copolymer Membranes

Modification of microfiltration membranes by hydrogel impregnation for pDNA purification

Intensified Downstream Processing of Monoclonal Antibodies Using Membrane Technology

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