Effect of Malnutrition on Cortisol-Binding Protein in the Lens of Cynomolgus Monkeys

Ono, Shigeru; Hirano, Hiroko; Uemura, Hiroyuki; Takahashi, Hiroshi; Obara, K; Hatakeyama, Tomiji; Wako, Hiroshi

doi:10.3177/jnsv.22.249

Cited by 2 publications

(1 citation statement)

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“…Protein malnutrition may also result in physiological and biochemical changes that directly affect drug interactions. A num ber of these changes may be involved in the alteration of tissue sensitivity to drugs [Varma, 1979], drug-metabolizing enzymes [Newberne et al, 1978], drug pharmacoki netics [Shastri and Krishnaswamy, 1976] and cell-hormone interactions [Ono et al, 1976]. Because caffeine exposure during pregnancy is extensive [Hill, 1973] and a large number of pregnant women do not meet the recommended daily allowance of protein [Zlatnik and Burmeister, 1983], one may wonder whether such nutritional stress can alter whatever susceptibility the growing fetal brain may have to caffeine.…”

Section: Introductionmentioning

confidence: 99%

Effect of Protein Malnutrition and Maternal Caffeine Intake on the Growth of Fetal Rat Brain

et al. 1987

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Pregnant dams were divided into two subgroups on day 10 of gestation. Half were fed a 20% protein diet and the other half an 8% protein diet. A second group also subdivided was pair-fed with rats of the first group. Their diet was supplemented with caffeine in amount calculated to provide daily doses of 2 mg/l00 g body weight. On days 18, 20, and 22 randomly selected dams were injected with 3H-thymidine intraperitoneally and 1.5 h later their fetuses were delivered surgically in order to determine the rate of DNA synthesis along the gestation. The rest of the fetuses were delivered surgically on day 22. Pups’ brains were rapidly removed and DNA, RNA, protein and ³H-thymidine uptake were studied. Average body weights of the fetuses in the caffeine-supplemented control group were smaller than those of the noncaffeine group. Effects of caffeine that were similar in both diet groups included a decrease in brain DNA content and concentration and an increase in brain protein content and concentration. However, the percent decrease and increase, respectively, was different depending on the nutritional status. DNA synthesis was not affected by malnutrition or caffeine supplementation on day 18 of gestation. Caffeine’s effect on the rate of DNA synthesis was different on day 20 of gestation depending on nutritional status. Caffeine supplementation resulted in a decrease in DNA synthesis in both groups on day 22 of gestation. These data indicate that caffeine intake during pregnancy produces differential effects on fetal rat brain depending on dietary protein content.

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