Low temperature (0-10ºC) is a key limiting factor for biochemical wastewater treatment methods [1]. The fact that varying cooling speed rate can cause different degrees of microbial cell damage has been studied. Rapid cooling causes intracellular ice damage, while slow cooling leads to microbial solution loss (solution damage) [2]. In a biological wastewater treatment process, the temperature variation rate is relative to the microbe, which has great influence on wastewater treatment efficiency [3-4]. Under normal temperatures, the generation and elimination of active oxygen in vivo keeps balance, while the active oxygen tends to excessive accumulation