Effect of magnetic field on the Eversa® Transform 2.0 enzyme: Enzymatic activity and structural conformation

Fraga, Fernanda Cristina; Valério, Alexsandra; Oliveira, Vanessa A.; Luccio, Marco Di; Oliveira, Débora de

doi:10.1016/j.ijbiomac.2018.10.171

Cited by 52 publications

(15 citation statements)

References 35 publications

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“…Unconventional approaches have been considered in order to enhance the catalytic capacity of soluble lipases. In an interesting work, Fraga et al [113] investigated the effects of a magnetic field on the enzymatic activity and structural conformation of the liquid lipase Eversa R Transform 2.0 R , aiming improve its stability and activity. Under a pH of 7.0, a magnetic field intensity of 1.34 T and 4 H of recirculation, the authors were able to obtain an enzyme activity 77% higher than the control sample.…”

Section: Enzymatic Hydroesterification: Use Of Soluble Lipasesmentioning

confidence: 99%

Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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Enzymatic synthesis of biodiesel showed advantageous characteristics in relation to other technologies once it works under bland conditions, no generation of wastewater, no occurrence of saponifications reactions and production of a biodiesel with high quality. Although many researches still apply immobilized lipases, the high costs associated with this biocatalyst hamper the economic viability of the process. Lipases in free/soluble/liquid formulation employed to biodiesel production via hydroesterification reaction have attracted interest from researchers because they are more cost effective than the immobilized form, making the enzymatic route more competitive. In addition, soluble lipases present higher reaction rates, reducing the time required to obtain a satisfactory biodiesel yield. Despite the fact that already exist industrial plants producing biodiesel with the assistance of lipases in liquid formulation, results of researches show that the process still needs to overcome some drawbacks. This paper is a comprehensive and critical discussion on the publications where soluble lipases were applied on biodiesel synthesis, as well as the challenges that the technology faces and its current status in pilot and industrial applications.

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Section: Enzymatic Hydroesterification: Use Of Soluble Lipasesmentioning

confidence: 99%

Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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“…No tratamento da lipase comercial Eversa® Transform 2.0 para o execução experimental foi utilizado um ímã permanente de neodímio borro-ferro com intensidade do fluxo magnético de 0,7 e 1,34 T. Neste trabalho foi utilizado o sistema de recirculação para analisar o efeito do campo ao tempo de exposição, com duração de 1, 2 e 4 horas. Com intuito de comprovar o efeito do campo na estrutura tridimensional da enzima este estudo procurou determinar a estrutura da enzima, a fim de verificar mudanças na estrutura secundária da proteína (FRAGA, et al, 2019). Diferente da metodologia de Fraga (2019), o estudo de lipases sobre a exposição do campo magnético verificou e comparou tanto a intensidade bem como a frequência do fluxo magnético na atividade enzimática, adotando o uso do campo magnético alternado (PRANDO, et al, 2018).…”

Section: Procedimentos Metodológicos Para Avaliar O Campo Magnético Nunclassified

Efeitos Do Uso Do Campo Magnético Na Atividade Enzimática in Vitro: Revisão Integrativa

Santos¹,

Oliveira²,

Porto³

et al. 2020

Ciência, Tecnologia E Inovação: Do Campo À Mesa

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Resumo Entre diversos tipos de biomoléculas, as enzimas possuem uma sensibilidade magnética ao ponto de modificar sua estrutura tridimensional. Essa modificação contribui no processo do encaixe-induzido da enzima ao substrato, e dependendo do tipo da classe enzimática e da intensidade do fluxo magnético pode haver uma diminuição ou aumento na atividade catalítica. Devido a isso, esse trabalho teve por objetivo sintetizar os trabalhos já publicados por meio de uma busca sistemática da literatura a fim de compreender quais enzimas já foram expostas ao tratamento do fluxo magnético para avaliar o efeito na atividade catalítica. Tratase de uma revisão integrativa. A busca foi realizada nas bases eletrônicas de dados Medline, Web of Science e Google scholar. Os critérios de elegibilidade foram artigos originais in vitro que utilizaram o campo magnético na atividade enzimática, publicada entre os anos de 2015 a 2020. Os artigos foram avaliados de acordo com os princípios de Bardín com análise do documento. Dos 69 artigos rastreados, participaram da revisão integrativa 08 artigos. Foi verificado artigos com resultados negativos e positivos. No entanto, a maioria teve efeito positivo, com aumento na atividade enzimática. Conclui-se que o campo magnético é uma alternativa para potencializar a atividade da enzima. Implicando em melhorar as condições reacionais na obtenção da enzima mais competitiva no setor industrial.

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“…Most of the published studies about Eversa lipases are transesterification of triglycerides or esterification of free fatty acids and used these enzymes in the free form [8][9][10][11][12]. Even though Eversa enzymes may be used in free form, their immobilization may enhance many properties such as stability and resistance to inhibitors chemicals [13].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Eversa Lipases on Hydrophobic Supports for Ethanolysis of Sunflower Oil Solvent-Free

Remonatto

Oliveira²,

Guisán

et al. 2022

Appl Biochem Biotechnol

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Lipases are an important group of biocatalysts for many industrial applications. Two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 was immobilized on four different hydrophobic supports: Lewatit-DVB, Purolite-DVB, Sepabeads-C18, and Purolite-C18. The performance of immobilized lipases was investigated in the transesterification of sunflower oil solvent-free in an anhydrous medium. Interesting results were obtained for both lipases and the four supports, but with Sepabeads support the lipases Eversa showed high catalytic activity. However, the more stable and efficient derivative was Eversa® Transform immobilized on Sepabeads C-18. A 98 wt% of ethyl ester of fatty acid (FAEE) was obtained, in 3 h at 40ºC, ethanol/sunflower oil molar ratio of 3:1 and a 10 wt% of the immobilized biocatalyst. After 6 reaction cycles, the immobilized biocatalyst preserved 70 wt% of activity. Both lipases immobilized in Sepabeads C-18 were highly active and stable in the presence of ethanol. The immobilization of Eversa Transform and Eversa Transform 2.0 in hydrophobic supports described in this study appears to be a promising alternative to the immobilization and application of these news lipases still unexplored.

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Effect of magnetic field on the Eversa® Transform 2.0 enzyme: Enzymatic activity and structural conformation

Cited by 52 publications

References 35 publications

Lipases in liquid formulation for biodiesel production: Current status and challenges

Lipases in liquid formulation for biodiesel production: Current status and challenges

Efeitos Do Uso Do Campo Magnético Na Atividade Enzimática in Vitro: Revisão Integrativa

Immobilization of Eversa Lipases on Hydrophobic Supports for Ethanolysis of Sunflower Oil Solvent-Free

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