Effect of Lutropin Concentration on the Efficiency of Isolated Porcine Kidney Storage in Modified Biolasol Solution

Ostróżka-Cieślik, Aneta; Dolińska, Barbara; Ryszka, Florian

doi:10.1016/j.transproceed.2020.01.100

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…It can be assumed that 0.1 µg/L PRL improves the behaviour of metabolic pathways and reduces the cell/tissue demand for energy [ 10 , 36 ]. Other studies also confirm the protective effect of sex hormones on nephrons in the period of ischemia [ 37 , 38 , 39 , 40 ]. The addition of 1 µg/L Zn 2+ to the modified Biolasol + PRL solution (group B3) caused a significant increase in the activity of enzymes in the kidney homogenates.…”

Section: Discussionmentioning

confidence: 56%

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

2021

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Zinc is an effective anti-inflammatory and antioxidant trace element. The aim of this study was to analyse the protective effect of zinc and zinc–prolactin systems as additives of preservation solutions in the prevention of nephron damage caused during ischemia. The study used a model for storing isolated porcine kidneys in Biolasol®. The solution was modified with the addition of Zn at a dose of 1 µg/L and Zn: 1 µg/L with prolactin (PRL): 0.1 µg/L. After 2 h and 48 h of storage, the levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, sodium, potassium, creatinine and total protein were determined. Zinc added to the Biolasol® composition at a dose of 1 µg/L showed minor effectiveness in the protection of nephrons. In turn, Zn2+ added to Biolasol + PRL (PRL: 0.1 µg/L) acted as a prolactin inhibitor. We do not recommend the addition of Zn(II) (1 µg/L) and Zn(II) (1 µg/L) + PRL (0.1 µg/L) to the Biolasol solution.

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Section: Discussionmentioning

confidence: 56%

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

2021

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“…The effectiveness of modified Biolasol ® in nephron protection was assessed based on the analysis of ALT and AST activities, total protein and urea concentrations as well as Na + and K + levels in the perfundates collected after starting and ending perfusion. A diagnostic tool was used to indirectly assess renal function [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. Analysis of the results obtained confirms the effect of selenium on ischemia-reperfusion injury of isolated porcine kidneys.…”

Section: Resultsmentioning

confidence: 99%

Therapeutic Potential of Selenium as a Component of Preservation Solutions for Kidney Transplantation

2020

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Selenium has strong antioxidant properties and diverse effects on the immune system. The aim of the study was to analyse the protective effect of selenium as a component of a kidney preservation solution on the prevention of ischemia-reperfusion injury of nephrons. The solution was modified by the addition of Se (1 µg/L), prolactin (0.1 µg/L) and Se with prolactin (1 µg/L Se + 0.1 µg/L PRL). The study used a model for storing isolated porcine kidneys in Biolasol® (modified Biolasol®), which minimizes ischemia-reperfusion injury of grafts. The introduction of Se4+ ions at a dose of 1 µg/L into the Biolasol® preservation solution in the form of Na2SeO3 caused an increase in the activity/concentration of the analysed biochemical parameters: aspartate transaminase, alanine transaminase, urea and protein. This suggests an adverse effect of Se4+ on nephron function during ischemia-reperfusion. The best graft protection was obtained by using Biolasol® modified with the addition of selenium (IV) at a dose of 1 µg/L and prolactin at a concentration of 0.1 µg/L. We proposed the mechanism of prolactin action in the metabolic reduction of selenite (SO32−) during ischemia/reperfusion.

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“…In our previous studies, we used Biolasol as a base to evaluate the effectiveness of pharmacological substances in flushing and preserving grafts. We have shown that prolactin (PRL, dose: 1 µg/L) [51,57], lutropin (LH, dose: 0.01 µg/L) [58], and selenium (Se 4+ , dose: 1 µg/L) [48] have a beneficial protective effect on the kidneys. The addition of Zinc to the Biolasol composition at a dose of 1 µg/L showed minor effectiveness in the protection of nephrons [59].…”

Section: Discussionmentioning

confidence: 99%

Effectiveness Assessment of a Modified Preservation Solution Containing Thyrotropin or Follitropin Based on Biochemical Analysis in Perfundates and Homogenates of Isolated Porcine Kidneys after Static Cold Storage

Ostróżka-Cieślik

Dolińska

Ryszka

2021

IJMS

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In this paper, we assess the nephroprotective effects of thyrotropin and follitropin during ischaemia. The studies were performed in vitro in a model of isolated porcine kidneys stored in Biolasol (FZNP, Biochefa, Sosnowiec, Poland) and modified Biolasol (TSH: 1 µg/L; FSH 1 µg/L). We used the static cold storage method. The study was carried out based on 30 kidneys. The kidneys were placed in 500 mL of preservation solution chilled to 4 °C. The samples for biochemical tests were collected during the first kidney perfusion (after 2 h of storage) and during the second perfusion (after 48 h of storage). The results of ALT, AST, and LDH activities confirm the effectiveness of Biolasol + p-TSH in maintaining the structural integrity of renal cell membranes. Significantly reduced biochemical parameters of kidney function, i.e., creatinine and protein concentrations were also observed after 48 h storage. The protective effect of Biasol + p-TSH is most pronounced after 2 h of storage, suggesting a mild course of damage thereafter. A mild deterioration of renal function was observed after 48 h. The results of our analyses did not show any protective effect of Biolasol + p-FSH on the kidneys during ischaemia.

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Effect of Lutropin Concentration on the Efficiency of Isolated Porcine Kidney Storage in Modified Biolasol Solution

Cited by 3 publications

References 16 publications

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

Biochemical Studies in Perfundates and Homogenates of Isolated Porcine Kidneys after Flushing with Zinc or Zinc–Prolactin Modified Preservation Solution Using a Static Cold Storage Technique

Therapeutic Potential of Selenium as a Component of Preservation Solutions for Kidney Transplantation

Effectiveness Assessment of a Modified Preservation Solution Containing Thyrotropin or Follitropin Based on Biochemical Analysis in Perfundates and Homogenates of Isolated Porcine Kidneys after Static Cold Storage

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