Effect of low‐power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts

Marques, Márcia Martins; Pereira, Aymann Nassif; Fujihara, Neusa A.; Nogueira, Fernando; Eduardo, Carlos

doi:10.1002/lsm.20008

Cited by 151 publications

(140 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…23,24 Conversely, increased cell proliferation rates have been observed for LLLT-treated cells at both energy doses selected for use in this study (0.5 and 3 J/cm 2 ), confirming that this type of therapy, at specific parameter levels, may biomodulate cell functions. [13][14][15][16][17][18]25,26 Most of the effects of LLLT have been associated with the activation of mitochondrial cytochrome c oxidase, which leads to increased adenosine triphosphate (ATP) production and the activation of several cell-signalling pathways. 27,28 In the present study, it may be speculated that similar laser biostimulation occurred in those irradiated fibroblasts that presented significantly increased proliferation (Table 3).…”

Section: Discussionmentioning

confidence: 99%

“…[9][10][11][12] Several researchers have demonstrated the biostimulatory effects of LLLT on different cell types, such as increased cell proliferation, migration, differentiation, and protein synthesis, as well as gene expression of collagen type I and growth factors. [13][14][15][16][17] Based on the positive effects of LLLT on bisphosphonate-induced osteonecrosis lesions and the toxic effects of these types of drugs on cells, the aim of the present study was to evaluate the proliferation and apoptosis rates of gingival fibroblasts exposed to ZA treatment and subjected to LLLT according to specific parameters.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid

Pansani

Basso

Turirioni

et al. 2014

International Journal of Oral and Maxillofacial Surgery

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid

Pansani

Basso

Turirioni

et al. 2014

International Journal of Oral and Maxillofacial Surgery

View full text Add to dashboard Cite

“…This therapy is able to restore the regular metabolic potential of stressed cells. [16][17] With this in mind, it could be posited that the initial irradiation on an already stressed tissue-as occurred in the therapeutic protocol group-may have led to a prompt response to the LPT, whereas the tissue that started to be irradiated when it was in a regular metabolic condition could have been inhibited using a relatively high dose of daily irradiation. Concurring with this hypothesis, França et al 13 have observed positive results in preventive protocols by using LPT in smaller dose.…”

Section: Discussionmentioning

confidence: 99%

Effect of laser phototherapy in the prevention and treatment of chemo-induced mucositis in hamsters

Lopez¹,

Martins²,

Pavesi

et al. 2013

Braz. oral res.

View full text Add to dashboard Cite

The aim of this study was to investigate the effect of laser phototherapy (LPT) in the prevention and/or treatment of oral mucositis induced by 5-fluorouracil (5-FU; Eurofarma, São Paulo, Brazil) in hamsters. Ninety-six hamsters were divided into four groups (n = 24): Control (no treatment); Preventive [LPT from day (D) D−5 to D+5]; Therapeutic (LPT from D+5 to D+15); and Combined (preventive plus therapeutic LPT from D−5 to D+15). The animals received an intraperitoneal injection of 5-FU on Days 0 and 2. The pouch mucosa was scratched on Days 3 and 4. The irradiation parameters were: indium-gallium-aluminum-phosphide (InGaAlP) diode laser (MM Optics, São Carlos, Brazil) (660 nm), beam area of 0.036 cm², 40 mW, 1.11 W/cm², 6.6 J/cm², power density applied daily of 39.6 J/cm², in punctual mode (six points and six seconds per point) and contact mode, one application per day. The animals were sacrificed on Days 0, 5, 10 and 15 (n = 6) and weighed, and the pouch mucosa was removed for histopathological analysis. Clinical and corresponding histological scores were compared using ANOVA and Tukey's test (p ≤ 0.05). Similar weight losses ranging from 5% to 10% occurred in all groups. The therapeutic group had significantly lower clinical and histological scores than the other groups at Day 10. This study showed that positive effects on oral mucositis management were obtained only when LPT was applied in the therapeutic protocol (from D+5 to D+15 after chemotherapy).

show abstract

“…It has been demonstrated that different cell types under stress conditions are more sensitive to the biomodulatory effect of laser irradiation [17][18][19][20]. The cell stress mechanisms may range from alterations in the oxidative agents and the temperature of the culture medium to nutritional deficit (decrease of fetal bovine serum (FBS) concentration in the culture medium), the latter being more effectively used to evaluate the effects laser irradiation on cell metabolism [13,17,[21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Tagliani¹,

Oliveira²,

Lins³

et al. 2010

Laser Phys. Lett.

View full text Add to dashboard Cite

In spite of knowing that cells under stress are biostimulated by low level laser (LLL) irradiation, the ideal condition of stress to different cell lines has not yet been established. Consequently, the aim of the present in vitro study was to evaluate the effects of a defined parameter of LLL irradiation applied on stressed odontoblast-like pulp cells (MDPC-23). The cells were seeded (12500 cells/cm 2 ) in wells of 24-well plates using complete culture medium (DMEM) and incubated for 24 hours. Then, the DMEM was replaced by a new medium with low concentrations (nutritional stress condition) of fetal bovine serum (FBS) giving rise to the following experimental groups: G1: 2% FBS; G2: 5% FBS; and G3: 10% FBS. The cells were irradiated three times with LLL in specific parameters (808±3 nm, 100 mW, 1.5 J/cm 2 ) every 24 hours. No irradiation was carried out in groups G4 (2% FBS-Control), G5 (5% FBS-Control), and G6 (10% FBS-Control). For all groups, the cell metabolism (MTT assay) and morphology (SEM) was evaluated. The experimental groups showed enhanced cell metabolism and normal cell morphology regardless of FBS concentration. A slight increase in the cell metabolism was observed only in group G2. It was concluded that cell nutritional stress caused by reducing the concentration of FBS to 5% is the most suitable method to assess the biostimulation of LLL irradiated MDPC-23 cells.MDPC-23 cells adhered to the glass substrate exhibited a spindle-shaped morphology and some mitosis were observed (arrows); SEM ×500

show abstract

Effect of low‐power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts

Abstract: Low-power laser irradiation causes ultrastructural changes in cultured fibroblasts. We suggest that these alterations may lead to disturbances in the collagen metabolism.

Cited by 151 publications

References 20 publications

Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid

Effects of low-level laser therapy on the proliferation and apoptosis of gingival fibroblasts treated with zoledronic acid

Effect of laser phototherapy in the prevention and treatment of chemo-induced mucositis in hamsters

Nutritional stress enhances cell viability of odontoblastlike cells subjected to low level laser irradiation

Contact Info

Product

Resources

About