Effect of low intensity laser interaction with human skin fibroblast cells using fiber-optic nano-probes

Pal, Gopalendu; Dutta, Ashim; Mitra, Kunal; Grace, Michael S.; Amat, Albert; Romanczyk, Tara B.; Wu, Xingjia; Chakrabarti, Kristi R.; Anders, Juanita J.; Gorman, Eric T.; Waynant, Ronald W.; Tata, Darrell B.

doi:10.1016/j.jphotobiol.2006.12.001

Cited by 59 publications

(29 citation statements)

References 37 publications

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“…Pal et al [39] found that the total energy dose had greater influence on cellular proliferation and the kinetics of reactive oxygen species generation than did laser intensity.…”

Section: Laser Therapymentioning

confidence: 99%

Selected physical medicine interventions in the treatment of diabetic foot syndrome

et al. 2016

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The diabetic foot syndrome (DFS) is among chronic complications of diabetes mellitus; it can affect individuals with both type 1 and type 2 diabetes. Diabetic patients have up to a 25% lifetime risk of developing DFS, which is both a medical and social problem. Several studies have indicated that, apart from pharmacotherapy and modern active wound dressings, physical medicine also has a role in prevention and management of diabetic foot ulcers. The paper presents physical medicine interventions most recognized in the conservative management of DFS.

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“…Pal et al [39] found that the total energy dose had greater influence on cellular proliferation and the kinetics of reactive oxygen species generation than did laser intensity.…”

Section: Laser Therapymentioning

confidence: 99%

Selected physical medicine interventions in the treatment of diabetic foot syndrome

et al. 2016

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“…Reactive oxygen species (ROS) can both activate NF-kB directly [16], and ROS are also involved in NF-kB activation by other stimuli such as tumor necrosis factor alpha (TNFa), phorbol ester, and interleukin (IL)-1 [17]. Several laboratories have observed the formation of ROS in cells in vitro after LLLT [18,19,20,21], and it has been proposed that ROS are involved in the signaling pathways initiated after photons are absorbed by the mitochondria within cells [22].…”

Section: Introductionmentioning

confidence: 99%

Low level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts

Chen

Arany

Huang

et al. 2009

Mechanisms for Low-Light Therapy IV

106

116

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“…чение пролиферации более чем в 2 раза в первые 3 сут, что авторы связали с активацией Са [50]. В некоторых работах [24][25][26] положительные результаты воздействия НИЛИ с длиной волны 635 нм достигаются при значительно большей ЭП 5 -Дж/см 2 , но касаются они стимулирования высво-бождения клетками эпидермального фактора роста (EGF).…”

Section: таблица 2 параметры нили-освечивания (лазерные излучающие гunclassified

The influence of continuous low-intensity laser radiation at the red (635 nm) and green (525 nm) wavelengths on the human mesenchymal stem cells in vitro: a review of the literature and original investigations

Moskvin

Klyuchnikov

Antipov

et al. 2016

Vopr. kurortol. fizioter. lech. fiz. kul't.

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32Имеющиеся к настоящему времени научные данные свидетельствуют о принципиальной воз-можности комплексного применения физиотера-певтических факторов и клеточных технологий в регенеративной медицине и открывают перспекти-вы для развития нового направления в современной биомедицине -регенеративной физиотерапии [1]. Мезенхимальные стволовые клетки (МСК) -муль-типотентные стромальные клетки -содержатся почти во всех тканях человека, способны к диффе- , работающие в непрерывном режиме. Фиксация лазерных излучающих головок обеспечивалась специальной насадкой (банкой) для лазерно-вакуумного массажа из комплекта КБ-5 диаметром 35 мм. Экспозиция во всех режимах составляла 5 мин. Выводы. Показано, что при используемых энергетиче-ских и временных параметрах воздействия морфология и жизнеспособность МСК не меняется. Полученные нами данные демонстрируют предельный уровень выбранных энергетических параметров, при которых наблюдается не просто отсут-ствие положительного эффекта в отношении стимулирования пролиферации, но, возможно, и ингибирование. Non-government educational facility of higher professional education «REAVIZ», Samara, Russia Low-intensity laser radiation can be used as one of the methods for the non-specific regulation of the human mesenchymal stem cell (MSC) activity at the preliminary stage of their in vitro cultivation. The objective of the present study was to estimate the influence of the limiting regimes of continuous low-intensity laser radiation (CLIR) of red (635 nm) and green (525 nm) spectra. Material and methods. The adhesive culture of human mesenchymal stem cells obtained from a donor's umbilical cord tissue was used in the experiments (following 4 passages). They were irradiated using a Lazmik-VLOK laser therapeutic device equipped with the KLO-635-40 (635 nm, 4,9 mW/cm 2 ) and KLO-525-50 (525 nm, 5,4 mW/cm 2 ) laser diode emitting heads operating in a continuous mode. A special nozzle (jar) for laser and vacuum massage (KB-5, 35 cm in diameter) was employed to fix the heads. The exposure time in all the irradiation regimes was 5 minutes. Conclusion. The study has demonstrated that neither the morphological features nor the viability of mesenchymal stem cells was altered under the influence of laser irradiation at the aforementioned energy and time parameters. The data obtained indicate that laser irradiation with the limiting levels of the chosen energy parameters produces no positive effect on the cell proliferative activity; more than that, it may cause its inhibition. Ключевые слова: мезенхимальные стволовые клетки, лазерное излучение, морфология и деление клеток. The influence of continuous low-intensity laser radiation of the red (635 nm) and red (525 nm) spectra on the human mesenchymal stem cells in vitro:

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Effect of low intensity laser interaction with human skin fibroblast cells using fiber-optic nano-probes

Cited by 59 publications

References 37 publications

Selected physical medicine interventions in the treatment of diabetic foot syndrome

Selected physical medicine interventions in the treatment of diabetic foot syndrome

Low level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts

The influence of continuous low-intensity laser radiation at the red (635 nm) and green (525 nm) wavelengths on the human mesenchymal stem cells in vitro: a review of the literature and original investigations

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