Effect of Lipopolysaccharide on Inflammation and Insulin Action in Human Muscle

Liang, Hanyu; Hussey, Sophie E.; Sanchez-Avila, Alicia; Tantiwong, Puntip; Musi, Nicolas

doi:10.1371/journal.pone.0063983

Cited by 152 publications

(133 citation statements)

References 41 publications

Supporting

Mentioning

126

Contrasting

Unclassified

Order By: Relevance

“…In addition, it has been shown that exercise decreases the expression of Toll-like receptor (TLR)2 and TLR4 in immune cells and skeletal muscle [73][74][75], while even short-term bed-rest increases the expression of TLR4 [66]. Importantly, activation of TLRs in skeletal muscle by factors such as lipopolysaccharide (LPS), heat shock protein 60 or NEFA has been suggested to be involved in the development of insulin resistance [73,76]. Moreover, because of exercise-induced increases in adrenaline (epinephrine), cortisol and other factors with immunomodulatory effects, physical activity may also have beneficial effects on immune system function [75].…”

Section: Myokines and Metabolic Regulationmentioning

confidence: 99%

Myokines in insulin resistance and type 2 diabetes

et al. 2014

View full text Add to dashboard Cite

Skeletal muscle represents the largest organ of the body in non-obese individuals and is now considered to be an active endocrine organ releasing a host of so-called myokines. These myokines are part of a complex network that mediates communication between muscle, the liver, adipose tissue, the brain and other organs. Recent data suggest that myokines regulated by muscle contraction may play a key role in mediating the health-promoting effects of regular physical activity. Although hundreds of myokines have recently been described in proteomic studies, we currently have a rather limited knowledge of the specific role these myokines play in the prevention of insulin resistance, inflammation and associated metabolic dysfunction. Several myokines are known to have both local and endocrine functions, but in many cases the contribution of physical activity to the systemic level of these molecules remains as yet unexplored. Very recently, novel myokines such as irisin, which is thought to induce a white to brown shift in adipocytes, have gained considerable interest as potential therapeutic targets. In this review, we summarise the most recent findings on the role of myokines in the regulation of substrate metabolism and insulin sensitivity. We further explore the role of myokines in the regulation of inflammation and provide a critical assessment of irisin and other myokines regarding their potential as therapeutic targets.

show abstract

Section: Myokines and Metabolic Regulationmentioning

confidence: 99%

Myokines in insulin resistance and type 2 diabetes

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Pyruvate oxidation was used to assess the activity of pyruvate dehydrogenase (PDH), the enzyme that catalyzes the oxidation of pyruvate and the provision of glucose-derived acetyl CoA to the TCA. [1][2][3][4][5][6][7][8][9][10][11][12][13][14] C]-pyruvate oxidation was assessed in a similar manner to fatty acid oxidation with the exception that pyruvate was substituted for BSA-bound palmitic acid. All samples were run in triplicate and data were normalized to total protein content and expressed in nmol/mg protein/h.…”

Section: Serum Endotoxin Was Measured With a Pyrogenementioning

confidence: 99%

“…Once in the circulation, endotoxin binds to the toll-like receptor 4 (TLR4) and activates several proinflammatory pathways (3). This modest elevation in circulating endotoxin, termed "metabolic endotoxemia" (2), has been implicated as a cause for the low-grade systemic inflammation observed in obesity and related metabolic diseases (2,4). Elevated circulating endotoxin has been shown to initiate obesity and insulin resistance in rodent models (4).…”

Section: Introductionmentioning

confidence: 99%

“…This modest elevation in circulating endotoxin, termed "metabolic endotoxemia" (2), has been implicated as a cause for the low-grade systemic inflammation observed in obesity and related metabolic diseases (2,4). Elevated circulating endotoxin has been shown to initiate obesity and insulin resistance in rodent models (4). In addition, elevated endotoxin concentrations have been associated with insulin resistance (4) and increased risk for incident type 2 diabetes in humans (5).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Probiotic supplementation attenuates increases in body mass and fat mass during high‐fat diet in healthy young adults

Osterberg

Boutagy

McMillan

et al. 2015

Obesity

View full text Add to dashboard Cite

Objective: The objective was to determine the effects of the probiotic, VSL#3, on body and fat mass, insulin sensitivity, and skeletal muscle substrate oxidation following 4 weeks of a high-fat diet. Methods: Twenty non-obese males (18-30 years) participated in the study. Following a 2-week eucaloric control diet, participants underwent dual X-ray absorptiometry to determine body composition, an intravenous glucose tolerance test to determine insulin sensitivity, and a skeletal muscle biopsy for measurement of in vitro substrate oxidation. Subsequently, participants were randomized to receive either VSL#3 or placebo daily during 4 weeks of consuming a high-fat (55% fat), hypercaloric diet (11,000 kcal day 21). Participants repeated all measurements following the intervention. Results: Body mass (1.42 6 0.42 kg vs. 2.30 6 0.28 kg) and fat mass (0.63 6 0.09 kg vs. 1.29 6 0.27 kg) increased less following the high-fat diet in the VSL#3 group compared with placebo. However, there were no significant changes in insulin sensitivity or in vitro skeletal muscle pyruvate and fat oxidation with the high-fat diet or VSL#3. Conclusions: VSL#3 supplementation appears to have provided some protection from body mass gain and fat accumulation in healthy young men consuming a high-fat and high-energy diet.

show abstract

“…Our data suggest that becoming sedentary leads to greater MT and endotoxemia, which in rodent models initiates reduced Inflammatory processes did not significantly influence the relationship between physical function and insulin sensitivity; however, microbial translocation covariates reduced the association to non-significance insulin signaling effectiveness and metabolic disease (Cani et al, 2007;Amar et al, 2011). This effect of endotoxin and insulin signaling has been replicated in human skeletal muscle myocytes and was reversible with blockade of LPS binding (Liang et al, 2013). Both being sedentary and insulin resistant contribute to reduced muscle anabolic signaling and permit loss of muscle mass, which sets the stage for development of frailty and its clinical consequences.…”

Section: Discussionmentioning

confidence: 61%

Microbial translocation and skeletal muscle in young and old vervet monkeys

Kavanagh

Brown

Davis

et al. 2016

AGE

View full text Add to dashboard Cite

Intestinal barrier dysfunction leads to microbial translocation (MT) and inflammation in vertebrate and invertebrate animal models. Age is recently recognized as a factor leading to MT, and in some human and animal model studies, MT was associated with physical function. We evaluated sarcopenia, inflammation, MT biomarkers, and muscle insulin sensitivity in healthy female vervet monkeys (6-27 years old). Monkeys were fed consistent diets and had large and varied environments to facilitate physical activity, and stable social conditions. Aging led to sarcopenia as indicated by reduced walking speeds and muscle mass, but general metabolic health was similar in older monkeys (n = 25) as compared to younger ones (n = 26). When older monkeys were physically active, their MT burden approximated that in young monkeys; however, when older monkeys were sedentary, MT burden was dramatically increased. MT levels were positively associated with inflammatory burden and negatively associated with skeletal muscle insulin sensitivity. Time spent being active was positively associated with insulin sensitivity as expected, but this relationship was specifically modified by the individual monkey's MT, not inflammatory burden. Our data supports clinical observations that MT interacts with physical function as a factor in healthy aging.

show abstract

Effect of Lipopolysaccharide on Inflammation and Insulin Action in Human Muscle

Cited by 152 publications

References 41 publications

Myokines in insulin resistance and type 2 diabetes

Myokines in insulin resistance and type 2 diabetes

Probiotic supplementation attenuates increases in body mass and fat mass during high‐fat diet in healthy young adults

Microbial translocation and skeletal muscle in young and old vervet monkeys

Contact Info

Product

Resources

About