Effect of lipoic acid on the oxidoreductive status of red blood cells in rats subject to oxidative stress by chronic administration of adriamycin

Abstract

<jats:p> One of the most intriguing phenomena observed during adriamycin (ADR) toxicity has been attributed to ADR-induced oxidative stress. The study was aimed to assess the protective effect of lipoic acid (LA) against ADR induced damage to erythrocytes. Male albino rats (Wistar strain) were subjected to ADR (1 mg/kg body weight/day i.v.) once a week for a period of 12 weeks. Haematological indices like haemoglobin levels (Hb) and haematocrit (Ht) were also lowered along with a marked increase in the activities of serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT). These rats demonstrated enhanced erythrocyte membrane lipid peroxidation (LPO) and an onslaught in the antioxidant defence armoury, witnessed by lowered activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), vitamin A, vitamin C and vitamin E. Rats administered with ADR showed a marked decline in the activities of membrane-bound ATPases. Abnormal LPO and decreased deformability led to increased osmotic fragility of the red blood cells. Pretreatment with LA (35 mg/kg body weight/day i.p.) 24 hours prior to the administration of ADR once a week for a period of 12 weeks was effective in counteracting these biochemical disturbances, thereby minimizing the toxic side effects of ADR. </jats:p>

Decrease in NADPH-Cytochrome P450 Reductase Activity of the Human Heart, Liver and Lungs in the Presence of Alpha-Lipoic Acid

“…An additional benefi cial effect of ␣ -lipoic acid on the heart was already reported in rats exposed to adriamycin [28,29] . A similar protective mechanism was observed in erythrocytes [30] and the kidney [31] .…”

Effect of Alpha Lipoic Acid in Treatment of Type 2 Diabetes

“…Our results show after ALA supplementation OS levels were reduced, which support other studies [57][58][59][60].…”

The Protective Effects of α-Lipoic Acid on Kidneys in Type 2 Diabetic Goto-Kakisaki Rats via Reducing Oxidative Stress

“…The decreased levels of these antioxidants in the diabetic control group was also an indication of increased oxidative stress in these kidneys. ALA improved the anti-oxidation ability in rats by regenerating vitamin C, vitamin E and SOD through an O-GlcNAc-dependent mechanism [12,14]. In this study, we found a decreased level of SOD in the diabetes control group which was possibly as a result of decreased anti-oxidation capabilities.…”

“…ALA quenches singlet oxygen, hydroxyl radicals, hypochloric acid, superoxide anion radicals, peroxyl radicals and hydrogen peroxide. ALA has metal-chelating activity and it is able to regenerate other natural antioxidants, such as vitamin C or vitamin E from their radical or inactive forms [13,14]. In light of the antioxidant activity of ALA, the purpose of this study was to verify oxidative stress in the pathogenesis of diabetic nephropathy in type 2 diabetic rats and to evaluate the protective effect of ALA on diabetic nephropathy.…”