Effect of lactic acid bacteria inoculants on fermentation characteristics and aerobic stability of three native grasses in Mongolian steppe

Abstract

Leymus chinensis, Leymus secalinus and Elymus nutans are three common grasses of the steppes of Central Asia. This study examined the effects of lactic acid bacteria on the fermentation qualities and aerobic stabilities of L. chinensis, L. secalinus and E. nutans silages. The forages were directly ensiled with or without lactic acid bacteria. Lactobacillus plantarum (LP), Lactobacillus brevis (LBR) and Lactobacillus buchneri NCIMB40788 (LBU) from a commercial inoculant were used as additives at 1.0 9 10 5 cfu g À1 of fresh matter. The control was sprayed with the same volume of distilled water. Six replicates from each treatment were ensiled in 3-L plastic buckets and stored at ambient temperature (~30°C). Fermentation quality and aerobic stability were determined after 60 days of ensiling. LP increased lactic acid content, lowered pH and decreased the content of butyric acid and ammonia nitrogen (P < 0.001) of the three silages. LBU increased the aerobic stabilities of the silages (P < 0.001). Lactobacillus brevis slowed the increase in pH, ammonia N content, and yeast count and slowed the decrease of lactic acid content during exposure to air. Overall, LP improved fermentation quality and LBU increased the aerobic stabilities of the native grass silages. Lactobacillus brevis reduced aerobic spoilage of L. chinensis and L. secalinus silages.

Effects of Malic Acid and Sucrose on the Fermentation Parameters, CNCPS Nitrogen Fractions, and Bacterial Community of Moringa oleifera Leaves Silage

“…fallax was found to proliferate at the heterofermentative stage [ 64 ]. Compared with homofermentative fermentation, heterofermentative fermentation is generally characterized by a higher pH, a high AA, and a lower LA content [ 65 ]. In the present study, both L. parabrevis and Le.…”

Effect of isolated lactic acid bacteria on the quality and bacterial diversity of native grass silage

“…Moreover, these steppe types are tolerant to cold, drought, sand, and biotic stresses ( Pang et al., 2011 ; Yuan et al., 2012 ; Fu et al., 2014 ), and play a key role in meeting the needs of animal production and, indirectly, protecting human health ( Wang et al., 2022 ). Traditionally, these steppes have been commonly used to make hay or as pasture for grazing ruminants ( Zhang et al., 2018 ). Generally, native grass hay is the traditional method of native grass; however, because the quality and palatability of natural hay is sensitive to environmental factors, it is not feasible to address the seasonal and annual imbalances through hay preparation techniques ( Li et al., 2015 ).…”