Effect of Lactate Fermentation Type on the Biochemical Methane Potential of Tequila Vinasse

Diaz-Cruces, Víctor F.; García‐Depraect, Octavio; León-Becerril, Elizabeth

doi:10.1007/s12155-020-10093-z

Cited by 29 publications

(11 citation statements)

References 39 publications

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“…Under the optimal conditions for hydrogen production (PBR1) the most abundant taxons in the microbial communities were: Clostridiaceae with the dominant genus Clostridium ( C. pasteurianum, C. tyrobutyricum, C. kluyveri, C. acetobutylicum ), Lactobacillaceae with Lactobacillus ( L. uvarum, L. plantarum, L. aquaticus, L. sucicola, L. brevis ), Ruminococcaceae , Prevotellaceae with Prevotella and Bifidobacteriaceae with Bifidobacterium . Many studies describing optimal conditions for hydrogen production and analyzing structures of the respective DF microbial communities (set out in Table 3 ) clearly show similar tendencies, especially regarding the contribution of LAB, HPB and additionally acetate-producers ( Hung et al, 2007 ; Yang et al, 2007 ; Etchebehere et al, 2016 ; García-Depraect et al, 2017 , 2019a , 2019b , 2019c ; García-Depraect and León-Becerril, 2018 ; Palomo-Briones et al, 2018 ; Fuess et al, 2019 ; Diaz-Cruces et al, 2020 ). The balance between putative HPB and LAB with the tendency for the former to prevail is typical for the most optimal conditions of hydrogen production in the majority of cases.…”

Section: Discussionmentioning

confidence: 88%

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

et al. 2021

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This study describes the dynamics and complexity of microbial communities producing hydrogen-rich fermentation gas from sugar-beet molasses in five packed-bed reactors (PBRs). The bioreactors constitute a part of a system producing hydrogen from the by-products of the sugar-beet industry that has been operating continuously in one of the Polish sugar factories. PBRs with different working volumes, packing materials, construction and inocula were tested. This study focused on analysis (based on 16S rRNA profiling and shotgun metagenomics sequencing) of the microbial communities selected in the PBRs under the conditions of high (>100 cm3/g COD of molasses) and low (<50 cm3/g COD of molasses) efficiencies of hydrogen production. The stability and efficiency of the hydrogen production are determined by the composition of dark fermentation microbial communities. The most striking difference between the tested samples is the ratio of hydrogen producers to lactic acid bacteria. The highest efficiency of hydrogen production (130–160 cm3/g COD of molasses) was achieved at the ratios of HPB to LAB ≈ 4:2.5 or 2.5:1 as determined by 16S rRNA sequencing or shotgun metagenomics sequencing, respectively. The most abundant Clostridium species were C. pasteurianum and C. tyrobutyricum. A multiple predominance of LAB over HPB (3:1–4:1) or clostridia over LAB (5:1–60:1) results in decreased hydrogen production. Inhibition of hydrogen production was illustrated by overproduction of short chain fatty acids and ethanol. Furthermore, concentration of ethanol might be a relevant marker or factor promoting a metabolic shift in the DF bioreactors processing carbohydrates from hydrogen-yielding toward lactic acid fermentation or solventogenic pathways. The novelty of this study is identifying a community balance between hydrogen producers and lactic acid bacteria for stable hydrogen producing systems. The balance stems from long-term selection of hydrogen-producing microbial community, operating conditions such as bioreactor construction, packing material, hydraulic retention time and substrate concentration. This finding is confirmed by additional analysis of the proportions between HPB and LAB in dark fermentation bioreactors from other studies. The results contribute to the advance of knowledge in the area of relationships and nutritional interactions especially the cross-feeding of lactate between bacteria in dark fermentation microbial communities.

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Section: Discussionmentioning

confidence: 88%

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

et al. 2021

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“…Those high concentrations of lactate and acetate are suggestive of heterolactic fermentation, which would happen during the collection, handling, or even storage of the TV. Indeed, earlier reports have shown diverse feedstocks with high concentrations of lactate and acetate [47][48][49]. In the literature, the presence of LAB in DF is a cause of controversy due to its different effects on hydrogen production [10,50,51].…”

Section: Effect Of the Inoculum Type On The Metabolic Profilesmentioning

confidence: 99%

Dark Fermentation Process Response to the Use of Undiluted Tequila Vinasse without Nutrient Supplementation

et al. 2021

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The technical feasibility of valorizing tequila vinasse (TV), a wastewater with high pollution potential, through the production of biogenic hydrogen via dark fermentation, has long been proven in diverse lab-scale reactors that were operated either in batch or continuous mode. However, such systems have mainly been tested with diluted streams and nutrient supplementation, hindering the techno-economic attractiveness of the TV-to-hydrogen concept at large scale. In this study, the feasibility of producing hydrogen from high-strength undiluted TV with no added extra nutrients was evaluated under batch mesophilic conditions. Additionally, the use of two different acidogenic inocula obtained either by heat or heat-aeration pretreatment was investigated to get a greater understanding of the effect of inoculum type on the process. The results obtained showed that the TV utilized herein contained macro- and micro-nutrients high enough to support the hydrogenogenic activity of both cultures, entailing average hydrogen yields of 2.4–2.6 NL H2/L vinasse and maximum hydrogen production rates of 1.4–1.9 NL H2/L-d. Interestingly, the consumption of lactate and acetate with the concomitant production of butyrate was observed as the main hydrogen-producing route regardless of the inoculum, pointing out the relevance of the lactate-driven dark fermentative process. Clostridium beijerinckii was ascertained as key bacteria, but only in association with microorganisms belonging to the genera Enterobacter and Klebsiella, as revealed by phylogenetic analyses.

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“…Therefore, it is imperative to optimize the first stage for the sake of achieving a superior global process performance in the two-stage AD of FW. In this context, lactate has been found to be the most appropriate substrate for methane formation compared to other acidogenic end-products such as butyrate or propionate (Pipyn and Verstraete, 1981;Wu et al, 2016;García-Depraect et al, 2020;Diaz-Cruces et al, 2020;Detman et al, 2021). From a bioenergetic viewpoint, lactate is the most energetically favorable acidogenic end-product for methanization (Pipyn and Verstraete, 1981).…”

Section: Introductionmentioning

confidence: 99%

Two-Stage Anaerobic Digestion of Food Waste: Enhanced Bioenergy Production Rate by Steering Lactate-Type Fermentation During Hydrolysis-Acidogenesis

et al. 2022

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Effect of Lactate Fermentation Type on the Biochemical Methane Potential of Tequila Vinasse

Cited by 29 publications

References 39 publications

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

Dynamics and Complexity of Dark Fermentation Microbial Communities Producing Hydrogen From Sugar Beet Molasses in Continuously Operating Packed Bed Reactors

Dark Fermentation Process Response to the Use of Undiluted Tequila Vinasse without Nutrient Supplementation

Two-Stage Anaerobic Digestion of Food Waste: Enhanced Bioenergy Production Rate by Steering Lactate-Type Fermentation During Hydrolysis-Acidogenesis

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