Effect of intradermal human recombinant copper‐zinc superoxide dismutase on random pattern flaps in rats

Schein, Ophir; Westreich, Melvyn; Shalom, Avshalom

doi:10.1002/hed.23114

Cited by 8 publications

(5 citation statements)

References 44 publications

(37 reference statements)

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“…In a murine model study, Ophir Schein reported increased ischemic flap viability using Cu-Zn SOD in intradermal injections 15 minutes before incisions at a dose of 50mg/kg. The group received intradermal Cu-Zn SOD presented viability in 49.18mm while control group in 34.84mm (p> 0.01) (38).…”

Section: Reactive Oxygen Species (Ros) Scavengersmentioning

confidence: 84%

Ischemic Skin Flaps: What to Use to Save Them? A Narrative Review

Oliveira¹,

Santana²,

Silva³

et al. 2023

Rev. Foco

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Skin flaps necrosis is an important complication in reconstructive surgeries and implies in more comorbidity to patients and higher costs due to and longer period of dressing and local wound care. This article intends to review physiology of non-ischemic and ischemic flaps and approach the main substances classes investigated to improve ischemic flaps viability. It’s fundamental understanding biochemical events that occur during flap surgery and how neurovascular effect, inflammatory response and reactive oxygen species (ROS) contributes to skin flap necrosis. Many substances have been researched to improve ischemic flaps viability, such as vasodilators, heparinoids, ROS scavengers and others. They presented good results, however most of them were studied in animal models only. Recent studies using gene therapy have obtained really excitant results, however in experimental models. Although a vast number of substances have been researched, Nitroglycerin has been tested in humans can be already used besides its side effects.

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Section: Reactive Oxygen Species (Ros) Scavengersmentioning

confidence: 84%

Ischemic Skin Flaps: What to Use to Save Them? A Narrative Review

Oliveira¹,

Santana²,

Silva³

et al. 2023

Rev. Foco

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“…Surgical procedures such as surgical delay, ischemic preconditioning, and flap prefabrication, as well as pharmacological agents such as sympatholytics, vasodilators, calcium channel blockers, anticoagulants, free radical scavenging antioxidants, and growth factors (bFGF, PDGF, TGF, VEGF), can be used for this purpose. (4)(5)(6)(7)(8).…”

Section: Discussionmentioning

confidence: 99%

“…Many techniques have been described to overcome necrosis, often requiring secondary surgical intervention. Various surgical procedures, including surgical delay, ischemic preconditioning, and flap prefabrication, as well as pharmacological agents like sympatholytic, vasodilators, calcium channel blockers, anticoagulants, free radical scavenging antioxidants, and growth factors (such as bFGF, PDGF, TGF, and VEGF), can be used for this purpose (4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

The effect of Ranolazine on random pattern skin flap survival in rats

Öztürk¹,

Koçer²,

Koca³

et al. 2023

Med Sci Discov

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Objective: Flap surgery is widely used in the field of plastic surgery for defect reconstruction. The most important problem of this surgery is ischemic necrosis which may occur in the distal of the flap. For this reason, studies have been conducted to develop many treatment methods to solve this problem. Our study aimed to investigate the effects of different doses of ranolazine, a selective late sodium current blocker, on the viability of skin flaps prepared from the back of the rat. Material and Methods: A total of 32 rats, including 8 rats in each group, were included in the study. A random patterned caudal base 3x9 cm width : length modified McFarlane skin flap was elevated from the backs of all rats and adapted back to its place For 7 days postoperatively, 1 cc of saline was added to the control group (Group A), and 45 mg/kg, 90 mg/kg, and 180 mg/kg of ranolazine were added to the study groups (Group B, C, D), respectively, and administered daily with gastric lavage On the 7th postoperative day, photographs were taken to evaluate the viability of the flaps and scintigraphic evaluations were made. After the rats were sacrificed, the flaps were separated from their bases and evaluated histopathologically. Results: As a result of the evaluations, the viable flap area was calculated as 12.7548 cm2 for Group A, 14.9533 cm2, 16.494 cm2 and 16.7599 cm2 for Groups B, C and D, respectively, and a statistically significant difference was found between the groups (P = 0.00 <0.05 ). The viable flap area percentages were calculated as 57.486% for Group A, 68.908% for Group B, 70.174% for Group C and 74.603% for Group D, and a statistically significant difference was found between the groups (P = 0.00 <0.05). As a result of scintigraphic evaluation, the viable flap area was found to be 61.57% for Group A, 71.04% for Group B, 70.21% for Group C and 73.85% for Group D, and a statistically significant difference was found between the groups (P = 0.03 <0.05). Histopathological evaluation revealed a decrease in the flaps' inflammation, edema and necrosis scores with the increasing drug dose. Conclusion: As a result, although flap viability increased with increasing doses in the group given ranolazine, more detailed studies are needed before it can be used clinically.

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“…Flep kayıplarını ortadan kaldırmak ve azaltmak için çok sayıda teknik tanımlanmıştır. İki basamaklı bir işlem olan cerrahi geciktirme (delay fenomeni), iskemik ön koşullandırma, vasküler implantasyon ile flep prefabrikasyonu kullanılabilmekle beraber pratik uygulamada daha çok sempatolitik, vazodilatör, kalsiyum kanal blokörü, antikoagülan, serbest radikal temizleyici antioksidan ilaçlar ve büyüme faktörleri (bFGF, PDGF, TGF ve VEGF) kan akımının artırılması ve neovaskülarizasyonun sağlanması için kullanılmıştır (2)(3)(4)(5)(6)(7)(8). Kullanılan diğer bir alternatif de tam kandan elde edilen trombositten zengin ürünlerdir (1,(8)(9)(10).…”

Section: Introductionunclassified

Konsantre büyüme faktörünün random patern cilt flep yaşayabilirliği üzerine etkisi: deneysel çalışma

Gürsoy

Koca

Alişik

et al. 2020

Journal of Health Sciences and Medicine

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Introduction: Random pattern skin flaps are used frequently for the reconstruction of random pattern skin defects from various causes in plastic and reconstructive surgery. Most frequent complication with these flaps is the necrosis of distal parts due to inadequate blood supply. Various types of techniques have been defined to avoid and reduce flap loss. The aim of this study is, for the first time, to evaluate the effect of concentrated growth factor (CGF), which is derived from whole blood and is rich in growth factors, on the survival of random pattern skin flaps. Material and Method: Fifty Sprague-Dawley rats were used for the study. Forty of them were used for the experiment group and ten for the preparation of CGF. Distal based 3x9 cm McFarlene flaps were elevated from the back of the rats and in CGF group (n:20), CGF matrix was placed before closure. No treatment was given to the control group (n:20). On postoperative day 7, the flaps were evaluated by photographic, scintigraphic, and histopathological methods. Results: The median rate of surviving flap areas in control group was 53% (47-58). In CGF group the median rate of flap viability was %58 (55-64). The higher flap viability rates obtained from CGF applied group was found to be statistically significantly higher from control group (p<0,001). For the scintigraphic perfusion area evaluations, the median rate of flap perfusion was 54% (51-60) for the control group and 61% (59-64) for the CGF group. The higher flap perfusion rates obtained from CGF applied group was found to be statistically significantly higher from control group (p=0,001). The median rate of vascularity scores in CGF treated group was 10,0 (8,25-11,75) and 7,5 (6,25-9,00) in control group. The difference was found to be statistically significantly higher in the study group (p=0,003). Inflammation scores were not found to be statistically significantly different (p=0,246). Conclusions: This study shows that locally applied CGF under skin flap increases flap viability which might be associated with the increase in angiogenesis. This study for the first time shows that in flap surgery, applying CGF which is rich in growth factors, might have favorable effects on the flaps that are prone to ischemia but further studies are needed.

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Effect of intradermal human recombinant copper‐zinc superoxide dismutase on random pattern flaps in rats

Abstract: The beneficial effect of HR-CuZnSOD on flap survival is attained when it is given intradermally into the flap tissue. Theoretically, Hr-CuZnSOD delivered with local anesthetics used in flap elevation may be a valuable clinical tool.

Cited by 8 publications

References 44 publications

Ischemic Skin Flaps: What to Use to Save Them? A Narrative Review

Ischemic Skin Flaps: What to Use to Save Them? A Narrative Review

The effect of Ranolazine on random pattern skin flap survival in rats

Konsantre büyüme faktörünün random patern cilt flep yaşayabilirliği üzerine etkisi: deneysel çalışma

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