Effect of insulin-like growth factor-I on in vitro maturation, fertilization and early embryonic development of cattle oocytes

Abstract

The present experiment was conducted to investigate the effect of insulin-like growth factor-I on in vitro maturation, fertilization and early embryonic development of cattle oocytes. A total of 318 nos. bovine ovaries were collected from slaughter houses for one year and 1089 nos. culturable oocytes were collected. 357 out of 1089 nos. culturable oocytes, were subjected to IGF-I supplemented serum and serum free basic maturation media. There was no significant difference in respect of maturation, fertilization and embryonic development between IGF-I supplemented serum and serum free basic maturation as well as culture media. The mean percentage of in-vitro maturation of bovine oocytes (IVM) based on cumulus cell expansion was found to be significantly higher (P<0.05) in serum basic maturation media without IGF-I (75.43 ± 3.25) than the serum free basic maturation media with IGF-I (67.27 ± 6.33) respectively. The mean cleavage percentages at 4-cell, 8-cell, 16-cell, morula & blastocyst stages of embryos in serum culture (without IGF-I) and serum free culture media (with IGF-I) were recorded as 47.