Effect of incorporation of triphala into AH26 sealer on its cytotoxicity at different intervals

Abstract

Background. One of the essential properties of sealers used during endodontic treatment is their biocompatibility. Different materials are added to these sealers to improve their properties, including antibacterial activity. In recent years, there has been an increase in interest in the use of herbal medicines. This study aimed to evaluate the effect of incorporating triphala into AH26 sealer on its cytotoxicity on gingival fibroblasts at different intervals after mixing. Methods. In the present in vitro study, the cytotoxicity of AH26 sealer was evaluated once in its pure form and once after mixing it with triphala at 24-, 48-, and 72-hour, and 7-day intervals after mixing using the standard MTT assay protocol on gingival fibroblasts. Results. Two-way ANOVA was used to evaluate the effect of groups on the mean changes in cytotoxicity at different time intervals at a significance level of P<0.05. The results showed that the incorporation of triphala into the AH26 sealer did not increase or decrease its cytotoxicity (P=0.909). Besides, there was a decrease in cytotoxicity in both study groups. However, there was a relative increase in the sealers’ cytotoxicity in both groups in the first 72 hours (P<0.0001). Conclusion. Considering the well-established antibacterial properties of triphala in our previous study, the present study’s results showed that the incorporation of triphala into the AH26 sealer did not increase the cytotoxicity of the sealer. Therefore, it can be incorporated into the AH26 sealer to improve the other properties of the sealer, including its antibacterial activity.

Cytotoxicity and Mineralization Activity of Calcium Silicate-Based Root Canal Sealers Compared to Conventional Resin-Based Sealer in Human Gingival Fibroblast cells

“…Resin-based sealers like AH26 have been commonly applied in endodontics although, formaldehyde release by these sealers leads to cytotoxicity. Previous studies have shown that the maximum toxicity of this sealer is in the rst 24 hours, and the toxicity of this sealer decreases as the sealer sets (22). Aluminum is one of the elements that is found in high amounts in Endoseal and since the cytotoxicity and toxicity effects of aluminum on animals have been observed, this element can be considered as the cause of cytotoxicity induced by this sealer; The effect of zirconium oxide found in Ceraseal as an opaci er has been proven to induce the proliferation of broblasts and accelerate the reduction of in ammatory reactions, as well as the cytotoxic effects of dental materials containing bismuth have been demonstrated.…”