Effect of immersion cycles on growth, phenolics content, and antioxidant properties of Castilleja tenuiflora shoots

Valdez-Tapia, Raúl; Capataz-Tafur, Jacqueline; López-Laredo, Alma R.; Trejo‐Espino, José Luis; Trejo‐Tapia, Gabriela

doi:10.1007/s11627-014-9621-5

Cited by 15 publications

(7 citation statements)

References 27 publications

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“…However, changing immersion frequencies from every 4 h to 8h, vitrification did not occur. The same trend was observed by Valdz-Tapia et al [17], who found that those with the longest total daily immersion times (60 min every 24 h) resulted in the production of vitrification in Castilleja tenuiflora. In this study, it was indicated that longer immersion times induce vitrification, and also found that lower immersion frequency may prevent vitrification, but result in the production of vigorous plants.…”

Section: High Efficiency Of Propagation For Sagittaria Sagittifolia Usupporting

confidence: 85%

“…Valdez-Tapia et al [17] reported that low immersion frequency (every 24 h) produced better results than higher frequencies (every 3 h and 12 h) for Castilleja tenuiflora. Therefore, the results are consistent with Berthouly and Etienne [7], who reported that the optimum immersion frequencies differed among explants types and species.…”

Section: High Efficiency Of Propagation For Sagittaria Sagittifolia Umentioning

confidence: 99%

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High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Gao¹,

Lin²,

Luo³

et al. 2016

JAST-A

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Abstract:Although several studies have reported on the propagation of the Sagittaria sagittifolia to date, none of these methods have efficiently achieved the mass production of these plants. The present study aimed to investigate the propagation and growth of S. sagittifolia using a temporary immersion bioreactor system (TIBS) compared with conventional semi-solid and liquid culture. The effect of different immersion frequencies and immersion times together with supplementation of various plant growth regulators to the Murashige and Skoog (MS) medium was evaluated on shoot proliferation and plant growth. The results showed that the higher immersion frequency (every 6 h) and shorter immersion time (3 min and 10 min) in medium containing 4 mg/L BA and 0.1 mg/L NAA produced the highest multiplication rate (23), which are significantly higher than conventional semi-solid (3.6) and liquid (4.5) method, and the best plant growth parameter. While, the lower immersion frequency and longer immersion time (30 min every 12 h and 60 min every 24 h) induced vitrification and pollution rate in shoot tips 16.6% and 19%, 42% and 37%, respectively. There is distinct decrease in pollution rate (8.3%) in TIBS (10 min every 6 h) compared with the conventional semi-solid and liquid cultures. Medium containing 4 mg/L BA and 0.5 mg/L NAA using 10 min immersion every 6 h showed satisfaction at the rooting stage, with high shoot proliferation rate (21.6), 100% rooting and 94% plant survival. Therefore, applying TIBS in S. sagittifolia is an efficient method for scaling up the production of plantlets with high quality seedlings.

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Section: High Efficiency Of Propagation For Sagittaria Sagittifolia Usupporting

confidence: 85%

Section: High Efficiency Of Propagation For Sagittaria Sagittifolia Umentioning

confidence: 99%

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Gao¹,

Lin²,

Luo³

et al. 2016

JAST-A

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“…The production of phenolic compounds is the main goal of the vast majority of those studies (Andarwulan and Shetty, 1999;Lozovaya et al, 2000;Santos-Gomes et al, 2003;Gális et al, 2004;Lozovaya et al, 2006;Kouakou et al, 2007;Bairu et al, 2011;Cui et al, 2011;Krzyzanowska et al, 2011;Palacio et al, 2012;Szopa et al, 2013;Siu et al, 2014;Szopa and Ekiert, 2014;Yildirim and Turker, 2014). However, some of them are also focused on the study of the extract's bioactive properties, such as antioxidant (Grzegorczyk et al, 2007;Hakkim et al, 2007;Kovatcheva-Apostolova et al, 2008;Hussein et al, 2010;Amoo et al, 2012;Giri et al, 2012;Khateeb et al, 2012; 16 Barros et al, 2013;Bhagya and Chandrashekar, 2013;Cheniany et al, 2013;Madhu, 2013;Goyali et al, 2014;Lugato et al, 2014;Piątczak et al, 2014;Valdez-Tapia et al, 2014), antimicrobial (Hussein et al, 2010;Ncube te al., 2011;Zhao et al, 2011;Khateeb et al, 2012) and cytotoxic (Skorić et al, 2012) activities.…”

Section: Production Of Phenolic Compounds Through Plant Tissue Culturmentioning

confidence: 99%

Exploring plant tissue culture to improve the production of phenolic compounds: A review

Días

Sousa

Alves

et al. 2016

Industrial Crops and Products

231

162

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Plant tissue and organ culture has been extensively used from the beginning of the XX century for the study and comprehension of some primary biological mechanisms such as morphogenesis. However, with the increasing demand of the market for novel products derived from plants, in vitro culture became a reliable technique for the mass production of plant material. Moreover, the potential to use this technique for the production of some bioactive compounds, such as phenolic compounds, is immense since it allows the manipulation of the biosynthetic routes to increase the production and accumulation of specific compounds. This work intends to make a brief historical review of in vitro culture, highlighting its use for the production of bioactive compounds. Also, emphasizes the importance of phenolic compounds for the consumer as well reviews the metabolic pathways involved in its production in plant cells.Furthermore, it was carried out a comprehensive study on the work developed for the production of plant phenolic compounds in in vitro cultures, as well as on the type of elicitors used to increase of the same production; also a brief highlighting of the phenolic compounds which serve as elicitors. There are numerous reports directed to the production of phenolic extracts in in vitro plant cultures, however there is a lack in the production of individual phenolic compounds mainly due to the complexity of the biosynthetic routes and extraction procedures. Elicitation procedures are often used to increase the production of phenolics, archieving in most cases higher yields than in nonelicitated cultures. The increasing production of bioactive phenolic extracts/compounds allows for their further applicability, namely in the industry of functional foods or in pharmaceutical/medical fields.3

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“…For experiments, forty explants (21-day-old) of 2.5 cm size were transferred into a temporary immersion bioreactor system (RITA®) with 200 mL of B5 liquid medium (Gamborg et al 1968) and sucrose (0.09 M) without plant growth regulators. In each bioreactor the flow rate in the inlet air was 1 L min −1 and the immersion cycle was 5 min every 24 h. (Valdez-Tapia et al 2014). Nitrogen stress was induced by modifying the KNO 3 and (NH 4 ) 2 SO 4 basal concentration in B5 medium without altering the nitrate:ammonium ratio (24:1).…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

“…There are different systems that can be used for these purposes, one of them being temporary immersion bioreactors, which offer among other advantages the reduction of labour cost, reduction of vitrification compared with permanent immersion, complete renewal of atmosphere at each immersion and secondary metabolite enhancement (Ashraf et al 2013;Valdez-Tapia et al 2014). Secondary metabolites biosynthesis in plants varies depending on environmental stress, and the biosynthesis of these compounds can be stimulated by biotic and abiotic factors (Ramakrishna and Ravishankar 2011).…”

Section: Introductionmentioning

confidence: 99%

MORPHOGENESIS AND SECONDARY METABOLITES PRODUCTION IN THE MEDICINAL PLANT Castilleja tenuiflora Benth. UNDER NITROGEN DEFICIENCY AND STARVATION STRESS IN A TEMPORARY IMMERSION SYSTEM

Cortes¹

2017

Rev.Mex.Ing.Quim.

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G. Inei-Shizukawa, H. A. Velasco-Bedrán, G. F. Gutiérrez-López and H. Hernández-Sánchez Abstract Castilleja tenuiflora Benth, is a medicinal plant that synthesizes secondary metabolites such as phenylethanoid glycosides (PhGs), flavonoids, lignans, and iridoid glycosides with pharmacological properties. Nutrient deficiency stress in in vitro culturing of plants has been shown to be a good biotechnological strategy to stimulate secondary metabolism. Our aim was to evaluate morphogenesis (growth and development) and the production of secondary metabolites in C. tenuiflora shoots in response to stress by nitrogen (N) deficiency and starvation in temporary immersion bioreactors. Shoots were grown in B5 liquid medium with 25.74 mM (control), 1.32, 0.66 (deficiency) and 0 mM (starvation) N concentration. In all treatments, matter and shoot multiplication rate (SMR) decreased over 40%, chlorophylls content decreased, roots length increased, and leaves showed chlorosis respect to control. Under 0.66 mM N deficiency, the enzyme phenylalanine ammonia lyase (PAL) was more active (twice more than control) and total phenolic compounds were enhanced. At day 21: maximum concentration of PhGs (verbascoside and isoverbascoside) and iridoids (aucubin) was observed in 0.66, 1.32 and 0 mM N treatments (283 ± 0.5, 90 ± 0.3 and 12.5 ± 0.1 mg g −1 DM, respectively); these concentrations were 175, 143 and 34% greater than respective controls on the same day. Additionally, presence of anthocyanins was evident in stems of all plants developed under N deficiency and starvation. Despite the fact that, the matter, SMR and chlorophylls decreased because N stress deficiency, the production of secondary metabolites was enhanced, demonstrating that in vitro culture in temporary immersion bioreactors under suitable nutrients deficit such as N is an appropriate strategy to stimulate secondary metabolism in C. tenuiflora. Keywords: abiotic stress, Aucubin, nitrogen starvation, phenylethanoid glycosides, RITA® bioreactor. ResumenCastilleja tenuiflora Benth., es una planta medicinal que sintetiza metabolitos secundarios como feniletanoides glicosilados (PhGs), flavonoides, lignanos, e iridoides; que presentan actividad farmacológica. El estrés por deficiencia de nutrientes en el cultivo de plantas in vitro ha mostrado ser una estrategia biotecnológica apropiada para estimular su metabolismo secundario. En este trabajo se evaluaron la morfogénesis (crecimiento y desarrollo) y la producción de metabolitos secundarios en brotes de C. tenuiflora en biorreactores de inmersión temporal expuestos a estrés por deficiencia y ausencia de nitrógeno (N). El estrés se aplicó a un cultivo de los brotes cultivados en medio líquido B5 con 25.74 mM (control), 1.32, 0.66 (deficiencia) y 0 mM (ausencia) de N. En comparación con el control, todos los tratamientos mostraron una disminución de más del 40% en la biomasa y elíndice de multiplicación de brotes, el contenido de clorofila disminuyó, la longitud de las raíces aumentó y las hojas mostraron clorosis. En defi...

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Effect of immersion cycles on growth, phenolics content, and antioxidant properties of Castilleja tenuiflora shoots

Cited by 15 publications

References 27 publications

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Exploring plant tissue culture to improve the production of phenolic compounds: A review

MORPHOGENESIS AND SECONDARY METABOLITES PRODUCTION IN THE MEDICINAL PLANT Castilleja tenuiflora Benth. UNDER NITROGEN DEFICIENCY AND STARVATION STRESS IN A TEMPORARY IMMERSION SYSTEM

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