Effect of If Current Blockade on Newborn Rat Heart Isolated According to Langendorff

Ziyatdinova, N. I.; Kuptsova, A. M.; Faskhutdinov, Lenar M.; Galieva, Alina Maratovna; Зефиров, А. Л.; Зефиров, Т. Л.

doi:10.1007/s10517-019-04541-w

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“…These include a switch from cardiomyocyte proliferation to cellular hypertrophy, a change in cardiomyocyte substrate utilization, an increase in myocardial oxidative capacity, and a metabolic shift from anaerobic glycolysis to aerobic respiration [8 , 9] . Prior works using newborn rats, lambs, and rabbits have leveraged the Langendorff-perfused neonatal heart preparation to provide valuable insight into developmental cardiac biology and pathophysiology [10] , [11] , [12] . However, an isolated-perfused heart model in the newborn mouse has yet to be established.…”

Section: Brief History and Rationalementioning

confidence: 99%

An isolated retrograde-perfused newborn mouse heart preparation

et al. 2020

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The Langendorff-perfused model is a powerful tool to study biological responses in the isolated heart in the absence of confounders. The model has been adapted recently to enable study of the isolated mouse heart and the effects of genetic manipulation. Unfortunately, the small size and fragility of the mouse heart pose significant challenges, limiting application of the Langendorff model to the study of adult mice. Cardiac development is a complex and dynamic process that is incompletely understood. Thus, establishing an isolated-perfused heart model in the newborn mouse would be an important and necessary advance. Here we present a method to successfully cannulate and perfuse the isolated newborn murine heart. We describe the basic and fundamental physiological characteristics of the ex-vivo retrograde-perfused beating neonatal heart in wild-type C57Bl/6 male mice. Our approach will enable future study of the physiological and pharmacological responses of the isolated immature murine heart to enhance knowledge of how developmental cardiac biology impacts health and disease. The Langendorff model is a powerful tool to study the heart without confounders. An isolated-perfused newborn murine heart model has yet to be established. We demonstrate the first successful isolated neonatal murine heart preparation.

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