Effect of hypertension on bacteria composition of prostate biopsy in patients with benign prostatic hyperplasia and prostate cancer in PSA grey-zone

Abstract

Abstract. Diagnostic prostate cancer (PC) is difficult to diagnose by prostate biopsy, even in patients with markedly elevated PSA levels. Therefore, we aimed to identify a new, better technique to detect PC in a more consistent manner. A variety of steps were employed to validate this proposed method, including DNA extraction, polymerase chain reaction (PCR) amplification, denaturing gradient gel electrophoresis (DGGE) and DGGE band sequencing. Four transperineal prostate biopsy specimens were obtained from male patients. The patients were under the age of 65 and PSA levels were 4-10 ng/ml. We also investigated the bacteria composition of transperineal prostate biopsy in patients with benign prostatic hyperplasia (BPH) and PC by PCR-DGGE profiling. Sequences from selected bands 2 and 4 both matched with Sphingomonas, which is present in lower amounts in PC without hypertension as compared to PC with hypertension, while there were no particular differences in the BPH group. Specific bacteria from the prostate biopsy tissues provide further confidence in PC diagnosis based on a PCR approach as a diagnostic tool, while hypertension was found to be a disturbing factor that can affect the diagnosis of BPH and PC in grey-zone.

P. acnes-Driven Disease Pathology: Current Knowledge and Future Directions

“…Inflammation is attributed to the presence of specific biomarkers such as elevated interleukin (IL)-6, tumor necrosis factor alpha (TNFα) and the acute phase protein, C reactive protein (Mechergui et al, 2009 ; Menschikowski et al, 2013 ; Yu et al, 2015 ). Recent work on urological fluids (i.e., urine, seminal fluid, prostatic secretions) as well as prostate biopsies suggests a significant conditional shift toward certain microbial species which may be used as a diagnostic index (Yu et al, 2015 ; Ni et al, 2016 ).…”

Astaxanthin Inhibits PC-3 Xenograft Prostate Tumor Growth in Nude Mice

“…The sequencing results showed that HA-treated tumor was rich in Lachnospiraceae and Lactobacillus sp. Previous studies showed that the microbial population in EPS, urine, seminal fluid, and prostate biopsy in PCa patients were significantly different, indicating correlation between PCa and BPH with microbiota [38]. Several studies had reported that lactobacilli enhance natural killer cell (NK) activity to effectively attack malignant tumor cells in vitro and in vivo [39,40,41].…”

“…However, there have been few studies on the effect of Lactobacillus on prostate cancer. Intestinal flora is related to human health and immunity, but whether there is any connection between urinary tract infection and intestinal flora—since urinary tract infection will change bacterial flora, which we had reported before [37,38]—had not been investigated previously. Whether Lactobacillus sp.…”