Effect of Hypergravity on the Level of Heat Shock Proteins 70 and 90 in Pea Seedlings

Kozeko, Liudmyla; Kordyum, Е.L.

doi:10.1007/s12217-008-9044-1

Cited by 15 publications

(6 citation statements)

References 14 publications

(15 reference statements)

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“…Based on this data, we speculate bioactive concentration window for endogenous SA to induce root growth is critical, and this may have played role in differential response to 10 ×g for 12 and 24 h. Alternatively, robust changes in jasmonate and SA at 10 ×g for 12 and 24 h could be in response to hypergravity-induced physiological stress rather than a direct correlation with root phenotype. Because, jasmonate and SA alone or in combination are known to ameliorate stress conditions 60 , and hypergravity on the other hand is reported to induce such physiological stress in living organism/s including plants 61 .…”

Section: Discussionmentioning

confidence: 99%

Novel hypergravity treatment enhances root phenotype and positively influences physio-biochemical parameters in bread wheat (Triticum aestivum L.)

Swamy

Hosamani

Sathasivam

et al. 2021

Sci Rep

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Hypergravity—an evolutionarily novel environment has been exploited to comprehend the response of living organisms including plants in the context of extra-terrestrial applications. Recently, researchers have shown that hypergravity induces desired phenotypic variability in seedlings. In the present study, we tested the utility of hypergravity as a novel tool in inducing reliable phenotype/s for potential terrestrial crop improvement applications. To investigate, bread wheat seeds (UAS-375 genotype) were subjected to hypergravity treatment (10×g for 12, and 24 h), and evaluated for seedling vigor and plant growth parameters in both laboratory and greenhouse conditions. It was also attempted to elucidate the associated biochemical and hormonal changes at different stages of vegetative growth. Resultant data revealed that hypergravity treatment (10×g for 12 h) significantly enhanced root length, root volume, and root biomass in response to hypergravity. The robust seedling growth phenotype may be attributed to increased alpha-amylase and TDH enzyme activities observed in seeds treated with hypergravity. Elevated total chlorophyll content and Rubisco (55 kDa) protein expression across different stages of vegetative growth in response to hypergravity may impart physiological benefits to wheat growth. Further, hypergravity elicited robust endogenous phytohormones dynamics in root signifying altered phenotype/s. Collectively, this study for the first time describes the utility of hypergravity as a novel tool in inducing reliable root phenotype that could be potentially exploited for improving wheat varieties for better water usage management.

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Section: Discussionmentioning

confidence: 99%

Novel hypergravity treatment enhances root phenotype and positively influences physio-biochemical parameters in bread wheat (Triticum aestivum L.)

Swamy

Hosamani

Sathasivam

et al. 2021

Sci Rep

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“…Figure 3A is an example of how a RGP experiment set-up could look like. In this study by Kozeko and Kordyum (2009) pea seedling (Pisum sativum) are exposed to various levels of hypergravity (HG) for 1 h each. After the HG exposure the samples are brought back to 1 g for either one or 24 h and the FIGURE 2 | Examples of reaching steady states.…”

Section: Examples Of the Rgp Hypothesismentioning

confidence: 99%

Centrifuges for Microgravity Simulation. The Reduced Gravity Paradigm

Loon

Jack²

2016

Front. Astron. Space Sci.

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Due to the cumbersome nature of performing real microgravity-spaceflight research scientists have been searching for alternatives to perform simulated microgravity or partial gravity experiments on Earth. For more than a century one uses the slow rotating clinostat as developed by von Sachs at the end of the nineteenth century. Since then, the fast rotating clinostat, the 3D clinostat or the random positioning machine, the rotating wall vessels, tail suspension and bed rest head down tilt and lately the levitating magnets have been introduced. Several of these simulation systems provide some similarities of the responses and phenotypes as seen in real microgravity experiments. However, one should always realize that we cannot reduce gravity on Earth, other than the relative short duration free fall studies in e.g., drop towers or parabolic aircraft. In this paper we want to explore the possibility to apply centrifuges to simulate microgravity or maybe better to simulate hypo-gravity. This Reduced Gravity Paradigm, RGP is based on the premise that adaptations seen going from a hypergravity level to a lower gravity are similar as changes seen going from unit gravity to microgravity.

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“…For example, accumulation of Heat Shock Proteins (HSPs) is crucial in protecting from thermal killing during heat stress (Vierling 1991). Kozeko and Kordyum have observed changes in the levels of HSPs under hypergravity (Kozeko and Kordyum 2009) and clinorotation (Kozeko and Kordyum 2006) in pea. Thus plants may adapt to the microgravity environment (real or simulated), which is also a stress, by synthesising required proteins.…”

Section: Effects Of Microgravity On Chlorophyll Contentmentioning

confidence: 99%

Effects of Clinorotation on Growth and Chlorophyll Content of Rice Seeds

Jagtap

Awhad

Santosh

et al. 2010

Microgravity Sci. Technol.

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Microgravity, as a different environment, has been shown to affect plant growth and development (Sievers et al. 1996;Sack 1997). In the present study, effects of slow clinorotation (2 rpm) on growth and chlorophyll content in rice (variety: PRH-10) seedlings were investigated. Rice seeds were clinorotated continuously for 3, 5 and 7 days under ambient conditions. Root and shoot lengths and weights of rice seedlings were measured on the third, fifth and seventh day. Chlorophyll was extracted using N, N-Dimethylformamide (DMF). Absorption and fluorescence spectra of chlorophyll were recorded. Chlorophyll a, chlorophyll b and total chlorophyll contents were calculated from absorption spectra using Arnon's method. Results showed an increase in root and shoot lengths in clinorotated samples. Similar results were obtained for root and shoot weights. Absorption spectra of chlorophyll showed no shift in the absorption peaks. Chlorophyll content was increased in clinorotated samples as compared to the controls. Interestingly, the difference between chlorophyll content in control and clinorotated samples decreased as the number of days of clinorotation increased. Chlorophyll a/b ratio was lowered in clinorotated samples as compared to the controls. These results suggest that slow clinorotation (2 rpm) affects plant growth and chlorophyll content in rice seedlings.

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Effect of Hypergravity on the Level of Heat Shock Proteins 70 and 90 in Pea Seedlings

Cited by 15 publications

References 14 publications

Novel hypergravity treatment enhances root phenotype and positively influences physio-biochemical parameters in bread wheat (Triticum aestivum L.)

Novel hypergravity treatment enhances root phenotype and positively influences physio-biochemical parameters in bread wheat (Triticum aestivum L.)

Centrifuges for Microgravity Simulation. The Reduced Gravity Paradigm

Effects of Clinorotation on Growth and Chlorophyll Content of Rice Seeds

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