Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells

Tunjung, Woro Anindito Sri; Yokoo, Masaki; Hoshino, Yumi; Miyake, Yuko; Kadowaki, Akane; Sato, Eimei

doi:10.1016/j.bbrc.2009.02.163

Cited by 18 publications

(21 citation statements)

References 26 publications

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“…The activation of caspases is a key factor in the signaling pathway that induces cell apoptosis, and the most important caspases are caspase-3, which is often referred to as the pre-caspase for the caspase cascade, and caspase-9 which is regarded as the execution caspase in cell apoptosis [31]. CD44, known to be a receptor for HA, constitutes a complicated system together with HAS-2 and HA for regulating cell apoptosis [32]. A study has revealed that knockdown of HAS-2 enhanced cell apoptosis by reducing HA content and CD44 expression and promoting caspase-3 expression, while overexpression of HAS-2 showed the opposite effect; the study also showed that HAS-2 partly inhibited the function of miR-26b in suppressing cell apoptosis [33].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

Zhang¹,

Feng²,

Wang³

et al. 2016

Cell Physiol Biochem

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Background: Breast cancer is characterized by a distinct metastatic pattern involving the regional lymph nodes, bone marrow, lung and liver. This study is aimed to investigate the effects of silencing the HAS-2 gene on the proliferation and apoptosis of human breast cancer cells. Methods: MCF-7 cells were collected and assigned into control, scrambled siRNA and HAS-2- siRNA groups. After transfection, the morphological changes in the MCF-7 cells were observed using phase contrast microscopy. qRT-PCR and Western blot assays were used to detect the mRNA and protein expression of apoptosis-related proteins. CCK-8 and flow cytometry were performed to evaluate cell proliferation, the cell cycle and apoptosis. Results: In the control and the scrambled siRNA groups, cells grew adhered to the wall and mainly showed a spindle shape with a clear nucleolus. Compared with the control and scrambled siRNA groups, increases in the number of cells in early apoptosis and metaphase cells in apoptosis were observed in the HAS-2-siRNA group. The HAS-2-siRNA group showed decreased expression of HAS-2 relative to that in the control and scrambled siRNA groups. No significant differences in cell proliferation, cell cycle distribution or apoptosis were noted between the control and scrambled siRNA groups. In the HAS-2-siRNA group, the cell proliferation ability decreased significantly, but the number of cells in the G0/G1 stage, the number of apoptotic cells and the expression of caspase-3 and caspase-9 increased significantly. Conclusion: Our findings indicate that HAS-2 gene silencing may inhibit proliferation and promote apoptosis in the MCF-7 human breast cancer cell line.

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Section: Discussionmentioning

confidence: 99%

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

Zhang¹,

Feng²,

Wang³

et al. 2016

Cell Physiol Biochem

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“…The degree of cumulus cells expansion if often cited as a major indicator oocyte selection for in vitro fertilization protocols (Yokoo et al, 2010). It has also been demonstrated that HA delays death and prevents fragmentation of porcine oocytes (Sato et al, 1994;Tunjung et al, 2009) and plays a role in cell migration during the early embryonic development (Peterson et al, 1993). Moreover, HA added to the culture medium supports the development of 1-and 2-cell porcine embryos (Miyano et al, 1994) as well as improves in vitro bovine embryo development to the blastocyst stage (Furnus et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Expression of CD44 in sheep oocytes and preimplantation embryos

Luz¹,

Alcântara-Neto²,

Batista³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in sheep oocytes and embryos. Immature oocytes (N = 66) were obtained from a local abattoir; mature oocytes (N = 35) and embryos (N = 41) were obtained by laparotomy from adult hair ewes submitted to ovarian stimulation treatment. The CD44 mRNA was detected by hemi-nested PCR, after reverse transcription, while proteins were located by indirect immunofluorescence, using anti-human CD44 monoclonal antibody. Human lymphocytes and immature bovine oocytes were used as positive and negative controls, respectively. Assessment of the oocyte nuclear stages as well as classification of the embryonic development stage were made with Hoechst 33342 staining. Indirect immunofluorescence detected CD44 expression on the surface of mature oocytes and embryos; immature oocytes did not take up the stain. These findings were supported by the RT-PCR data, which showed no mRNA templates for CD44, even after two consecutive amplifications, in material from immature oocytes and cumulus cells. The CD44 amplicons were detected after a second hemi-nested PCR in mature oocytes and embryos. The finding of CD44 in mature oocytes and preimplantational embryos could reflect the expression profile of hyaluronic acid during terminal folliculogenesis and preimplantational embryo development in sheep.

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“…MPF activation at the onset of meiotic resumption is inhibited by intra-oocyte cAMP, which is transferred from cumulus cells via gap junctional communication within COCs. Interruption of gap junctions in the COCs, which occurs in response to the pre-ovulatory surge of gonadotropins (Schoenfelder and Einspanier, 2003;Assidi et al, 2008;Tunjung et al, 2009), leads to a drop in the intra-oocyte concentration of cAMP, followed by MPF activation and meiotic resumptions. The reduction of the intra-oocyte cAMP concentration was suppressed by the inhibition of the interaction between hyaluronan and CD44.…”

Section: Discussionmentioning

confidence: 99%

“…The degree of cumulus cells expansion is often cited as a major indicator oocyte selection for in vitro fertilization protocols (Yokoo et al, 2010). It has also been demonstrated that HA delays death and prevents fragmentation of porcine oocytes (Sato et al, 1994;Tunjung et al, 2009) and plays a role in cell migration during the early embryonic development (Peterson et al, 1993). Moreover, HA added to the culture medium supports the development of 1-and 2-cell porcine embryos (Miyano et al, 1994) as well as improving in vitro bovine embryo development to the blastocyst stage (Furnus et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Expression of CD44 Gene in Goat’s Oocytes and Embryos

Aminafshar¹,

Bahrampour²,

Bagizadeh³

et al. 2014

GJBS

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The CD44 family belongs to a larger group of hyaluronic acid-binding proteins and plays important roles in oocyte maturation, fertilization and preimplantational embryo development. We analyzed the CD44 receptor in goat oocytes and embryos. Immature oocytes (N = 90) were obtained from a local abattoir; mature oocytes (N = 40) and embryos (N = 25) were obtained by laparotomy from adult hair ewes submitted to ovarian stimulation treatment. The CD44 mRNA was detected by PCR, after reverse transcription, while proteins were located by indirect immunofluorescence, using anti-human CD44 monoclonal antibody. Human lymphocytes and immature bovine oocytes were used as positive and negative controls respectively. Assessment of the oocyte nuclear stages as well as classification of the embryonic Growth phase were made with Hoechst 33342 staining. Indirect immunofluorescence detected CD44 expression on the surface of mature oocytes and embryos; immature oocytes did not take up the stain. These findings were supported by the RT-PCR data, which showed no mRNA templates for CD44, in material from immature oocytes and cumulus cells. The CD44 amplicons were detected after a second heminested PCR in mature oocytes and embryos. The finding of CD44 in mature oocytes and preimplantational embryos could reflect the expression profile of hyaluronic acid during terminal folliculogenesis and preimplantational embryo development in goats.

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Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells

Cited by 18 publications

References 26 publications

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

Expression of CD44 in sheep oocytes and preimplantation embryos

Expression of CD44 Gene in Goat’s Oocytes and Embryos

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