Effect of hormonal manipulation and doxorubicin administration on cell cycle kinetics of human breast cancer cells

Abstract

Summary Dual-parameter flow cytometry, following bromodeoxyuridine (iL dUrd) incorporation and propidium iodide (P1) uptake into DNA, was used to study the effects of oestradiol and/or insulin on cell cycle kinetics of human breast cancer cells in vitro. After a lag-period of 6-12 h, an )ptimum in the percentage of S-phase cells was reached between 18 and 24 h after hormone administration. A 1 h pulse of oestradiol was as effective as the continuous presence of oestradiol in pushing the cells from quiescent growing cultures into the cell cycle. A I h pulse of insulin was less effective than continuous administration. The addition of doxorubicin resulted in an accumulation of the cells in the late S/G2M-phases. It is concluded that dual-parameter flow cytometry allows accurate assessment of the effects of hormones and chemotherapy on the cell cycle. Therefore this method is very suitable for studying the interaction of hormones and chemotherapy on cell growth.

A Focus on “Bio” in Bio–Nanoscience: The Impact of Biological Factors on Nanomaterial Interactions

“…[ 102 ] Similarly, estradiol has been used to increase the fraction of MCF‐7 cells in the S phase to improve the sensitivity of the breast cancer cell line to an S phase‐specific cytotoxic drug. [ 103,104 ] Roa et al used glucose‐capped AuNPs to improve the radiation sensitivity of DU‐145 prostate cancer cells that are typically resistant to radiation. It was reported that the NPs induced the accumulation of the cells in the G2/M phase, which are more sensitive to ionizing radiation.…”

A Focus on “Bio” in Bio–Nanoscience: The Impact of Biological Factors on Nanomaterial Interactions

“…[ 102 ] Similarly, estradiol has been used to increase the fraction of MCF‐7 cells in the S phase to improve the sensitivity of the breast cancer cell line to an S phase‐specific cytotoxic drug. [ 103,104 ] Roa et al used glucose‐capped AuNPs to improve the radiation sensitivity of DU‐145 prostate cancer cells that are typically resistant to radiation. It was reported that the NPs induced the accumulation of the cells in the G2/M phase, which are more sensitive to ionizing radiation.…”

Risk of Cancer in Diabetes: The Effect of Metformin

“…In summary, the proposed mechanisms explaining the possible positive link between hyperinsulinemia and cancer risk aredirect promotion of tumor proliferation [35],modulation of circulating growth factors and their binding proteins [36],binding to growth factor specific receptors [37]. …”

Effects of suramin on cell-cycle kinetics of MCF-7 human breast cancer cells in vitro

“…After this incubation cells were washed twice and harvested by trypsinisation, followed by the addition of 1 ml trypsin inhibitor (0.1 mg ml1; Sigma, St Louis, Mo, USA) in PBS. The cell pellets were stored in 2 ml of 70°C ethanol (-20C) before preparation for analysis of cell cycle distribution by dual-parameter (anti-bromodeoxyuridine-FITC/propidium iodide) flow cytometry, performed as described previously (Bontenbal et al, 1989). The results are expressed as mean ± intraexperimental standard errors or standard deviations.…”

A Focus on “Bio” in Bio–Nanoscience: The Impact of Biological Factors on Nanomaterial Interactions

“…[ 102 ] Similarly, estradiol has been used to increase the fraction of MCF‐7 cells in the S phase to improve the sensitivity of the breast cancer cell line to an S phase‐specific cytotoxic drug. [ 103,104 ] Roa et al used glucose‐capped AuNPs to improve the radiation sensitivity of DU‐145 prostate cancer cells that are typically resistant to radiation. It was reported that the NPs induced the accumulation of the cells in the G2/M phase, which are more sensitive to ionizing radiation.…”

Risk of Cancer in Diabetes: The Effect of Metformin

“…In summary, the proposed mechanisms explaining the possible positive link between hyperinsulinemia and cancer risk aredirect promotion of tumor proliferation [35],modulation of circulating growth factors and their binding proteins [36],binding to growth factor specific receptors [37]. …”

Effects of suramin on cell-cycle kinetics of MCF-7 human breast cancer cells in vitro

“…After this incubation cells were washed twice and harvested by trypsinisation, followed by the addition of 1 ml trypsin inhibitor (0.1 mg ml1; Sigma, St Louis, Mo, USA) in PBS. The cell pellets were stored in 2 ml of 70°C ethanol (-20C) before preparation for analysis of cell cycle distribution by dual-parameter (anti-bromodeoxyuridine-FITC/propidium iodide) flow cytometry, performed as described previously (Bontenbal et al, 1989). The results are expressed as mean ± intraexperimental standard errors or standard deviations.…”

A Focus on “Bio” in Bio–Nanoscience: The Impact of Biological Factors on Nanomaterial Interactions

“…[ 102 ] Similarly, estradiol has been used to increase the fraction of MCF‐7 cells in the S phase to improve the sensitivity of the breast cancer cell line to an S phase‐specific cytotoxic drug. [ 103,104 ] Roa et al used glucose‐capped AuNPs to improve the radiation sensitivity of DU‐145 prostate cancer cells that are typically resistant to radiation. It was reported that the NPs induced the accumulation of the cells in the G2/M phase, which are more sensitive to ionizing radiation.…”

Risk of Cancer in Diabetes: The Effect of Metformin

“…In summary, the proposed mechanisms explaining the possible positive link between hyperinsulinemia and cancer risk aredirect promotion of tumor proliferation [35],modulation of circulating growth factors and their binding proteins [36],binding to growth factor specific receptors [37]. …”

Effects of suramin on cell-cycle kinetics of MCF-7 human breast cancer cells in vitro

“…After this incubation cells were washed twice and harvested by trypsinisation, followed by the addition of 1 ml trypsin inhibitor (0.1 mg ml1; Sigma, St Louis, Mo, USA) in PBS. The cell pellets were stored in 2 ml of 70°C ethanol (-20C) before preparation for analysis of cell cycle distribution by dual-parameter (anti-bromodeoxyuridine-FITC/propidium iodide) flow cytometry, performed as described previously (Bontenbal et al, 1989). The results are expressed as mean ± intraexperimental standard errors or standard deviations.…”