Effect of high-molecular-weight glutenin subunit Dy10 on wheat dough properties and end-use quality

Yan, Wei; Guo, Zhen-Ru; CHEN, Qing; Yang, Li; Zhao, Kan; Wan, Yongfang; HAWKESFORD, Malcolm J.; Kong, Li; Pu, Zhien; Deng, Mei; Jiang, Qiantao; Lan, Xiujin; Wang, Jirui; CHEN, Guo-yue; Ma, Jian; Zheng, Youliang; Wei, Yuming; Qi, Pengfei

doi:10.1016/j.jia.2022.08.041

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…The glutenin and gliadin contents in whole wheat our samples were determined using an Agilent 1260 In nity system with the Zorbax 300SB-C18 stable bond analytical column (4.6 × 250 mm, 5 µm) (Agilent, Palo Alto, CA, USA). Experiments were conducted as described by Zheng et al (2018) and Wang et al (2023).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Fine-mapping and validation of Gpc-5B as the locus responsible for increases in the grain protein content, grain size, and spike density in common wheat (Triticum aestivum L.)

Li,

Zhu

et al. 2024

Preprint

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Grain protein content (GPC), grain size, and spike morphology are important traits for common wheat (Triticum aestivum) breeding. However, few genes positively regulating these three traits have been reported. In this study, the gene responsible for increases in GPC, grain size, and spike density in the mutant S-Cp2 were mapped and cloned. This mutant was obtained by treating the common wheat cultivar ‘Shumai482’ with ethyl methanesulfonate (EMS). According to a genetic analysis, the increases in GPC and grain size and the formation of compact spikes in S-Cp2 were controlled by a single dominant locus (Gpc-5B) on chromosome 5BL. This locus was mapped to an 87.24 kb interval between markers KASP-58070 and KASP-58158, wherein 5Bq (homologous to Q on chromosome 5AL) was detected. One missense mutation (G-to-A) was detected within the microRNA172-binding site of 5Bq in S-Cp2 (i.e., 5BQ4 allele), suggesting that 5Bq is responsible for the mutant phenotype. This was confirmed by cloning 5Bq in revertants obtained by treating S-Cp2 with EMS. As expected, 5BQ4 in S-Cp2 was significantly more highly transcribed than 5Bq in ‘Shumai482’. Notably, 5Bq was positively selected during breeding. The study findings reflect the value of 5Bq for breeding novel wheat varieties.

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Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Fine-mapping and validation of Gpc-5B as the locus responsible for increases in the grain protein content, grain size, and spike density in common wheat (Triticum aestivum L.)

Li,

Zhu

et al. 2024

Preprint

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“…The effect of subunit Dy10 on wheat dough properties and end-use quality was studied by generating and analyzing a deletion mutant with the Dy-null allele. The Dynull allele might be exploited by wheat breeding programs [149].…”

Section: Allelic Variation At Glu-d1 Locusmentioning

confidence: 99%

Allelic Variation of High-Molecular-Weight Glutenin Genes in Triticum Species and Triticale (× Triticosecale Wittmack)

Spetsov,

Daskalova

2024

OBM Genet

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High-molecular-weight glutenin subunits (HMW-GS) encoded by alleles at the Glu-A1, Glu-B1, and Glu-D1 loci confer unique bread-making properties of common wheat (Triticum aestivum L.). The identification of HMW-GS is the prerequisite for pyramiding high-quality glutenin genes. The present review is designed to list all published HMW-GS alleles in Triticum species and triticale (A- and B genomes), focusing on methods for their identification. T. monococcum is characterized by 37 alleles at the Glu-1 locus versus four alleles in T. thaoudar and 39 in T. urartu. In total, 80 alleles at Glu-A1 of diploid Triticum species and about 42 alleles found in polyploid wheat landraces and varieties (4x and 6x), including triticale, were listed. Allelic variation at the Glu-B1 locus is divided into 3 groups: a – z, aa – az, and ba – ct, comprising 121 alleles, of which 26 subunits have unspecified alleles. At least 51 allelic variants at locus Glu-D1 of Triticum species were indicated, along with carriers of the species level. In addition, subunit-specific genetic loci have been tagged, facilitating molecular marker development of high-gluten wheat cultivars through marker-assisted breeding.

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“…Grain with a higher content of HMW-GSs is characterized by higher values of the sedimentation index [10]. HMW-GSs are regarded as the critical determinants of the processing suitability of wheat grain [50][51][52][53] despite the fact that they account for only 5-10% of storage proteins in wheat kernels [4,54]. The endosperm of wheat kernels contains 20-30% LMW-GSs [4,54].…”

Section: Introductionmentioning

confidence: 99%

The Quality of Winter Wheat Grain by Different Sowing Strategies and Nitrogen Fertilizer Rates: A Case Study in Northeastern Poland

Lachutta,

Jankowski

2024

Agriculture

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The present study was undertaken to determine the effect of different sowing strategies and spring nitrogen (N) fertilizer rates on the technological quality of winter wheat (Triticum aestivum L.) grain in terms of its milling quality, protein complex quality, and enzyme activity (falling number). Winter wheat grain for laboratory analyses was produced in a small-area field experiment conducted between 2018 and 2021 in the AES in Bałcyny (53°35′46.4″ N, 19°51′19.5″ E, NE Poland). The experimental variables were (i) sowing date (early: 6 September 2018, 5 September 2019, and 3 September 2020; delayed by 14 days: 17–20 September; and delayed by 28 days: 1–4 October), (ii) sowing density (200, 300, and 400 live grains m−2), and (iii) split application of N fertilizer in spring (40 + 100, 70 + 70, and 100 + 40 kg ha−1) at BBCH stages 22–25 and 30–31, respectively. A sowing delay of 14 and 28 days increased the bulk density (by 1 and 1.5 percent points (%p), respectively), vitreousness (by 3 and 6%p, respectively), and total protein content of grain (by 1% an 2%, respectively). A sowing delay of 14 days increased grain hardness (by 5%), the flour extraction rate (by 1.4%p), and the falling number (by 3%) while also decreasing grain uniformity (by 1.9%p). In turn, a sowing delay of 28 days increased the wet gluten content of grain (+0.5–0.6%p) and improved the quality of the protein complex in the Zeleny sedimentation test (+1.5%). An increase in sowing density from 200 to 300 live grains m−2 led to a decrease in grain uniformity (by 2.6%p), the total protein content (by 1.5%), and the wet gluten content of grain (by 0.7%p). A further increase in sowing density decreased grain vitreousness (by 1.4%p). The grain of winter wheat fertilized with 40 and 100 kg N ha−1 in BBCH stages 22–25 and 30–31, respectively, was characterized by the highest hardness (64.7), vitreousness (93%), flour extraction rate (73.9%), total protein content (134 g kg−1 DM), wet gluten content (36%), and Zeleny sedimentation index (69 mL).

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Effect of high-molecular-weight glutenin subunit Dy10 on wheat dough properties and end-use quality

Cited by 3 publications

References 25 publications

Fine-mapping and validation of Gpc-5B as the locus responsible for increases in the grain protein content, grain size, and spike density in common wheat (Triticum aestivum L.)

Fine-mapping and validation of Gpc-5B as the locus responsible for increases in the grain protein content, grain size, and spike density in common wheat (Triticum aestivum L.)

Allelic Variation of High-Molecular-Weight Glutenin Genes in <i>Triticum</i> Species and Triticale (× <i>Triticosecale</i> Wittmack)

The Quality of Winter Wheat Grain by Different Sowing Strategies and Nitrogen Fertilizer Rates: A Case Study in Northeastern Poland

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