Effect of high doses of gamma radiation on the functional characteristics of amniotic membrane

Singh, Rita; Purohit, Sumita; Chacharkar, M. P.

doi:10.1016/j.radphyschem.2006.09.010

Cited by 16 publications

(10 citation statements)

References 13 publications

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“…16 A study with high-dose gamma radiation (25-50 kGy) on AM observed no qualitative changes of water absorption capacity, water vapor transmission rate, and impermeability to different microorganism. 17 In vitro study to inactivate HIV-1 by radiation demonstrated the presence of active virus replication after exposure to as much as 5.0 mrad, and after irradiation with 10.0 mrad, the sample indicated cellular damage. It seems that good screening procedures along with sterilization by gamma radiation might be the most effective means for providing the safe possible allografts.…”

Section: Discussionmentioning

confidence: 99%

Comparisons of the Effects of Biological Membrane (Amnion) and Silver Sulfadiazine in the Management of Burn Wounds in Children

Mostaque

Rahman²

2011

Journal of Burn Care & Research

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This prospective study was conducted on 102 children with second-degree thermal burns to assess qualitative differences between topical silver sulfadiazine (SD) and oven-dried, radiation-sterilized human amnion as wound dressing. The patients were divided into silver SD and amniotic membrane (AM) group by random sampling technique. The variables compared 1) the number of days admitted in the hospital, 2) the number of dressing changes, 3) time needed for epithelialization, 4) comfort and pain of the patients during dressing, 5) comfort and pain of the patients between dressings, 6) activities during treatment, 7) acceptability of the modules by the patients or attending guardians, and 8) comfort of the doctor during application. Patients' ages ranging from 1 day to 12 years and admitted to inpatient burn unit within 72 hours of occurrence were included in this study. Fifty-one burned children enrolled in each group. The mean hospital stay is significantly lower in AM group (P < .01). The number of dressing changes in AM group was significantly low (P < .001). The mean time taken for epithelial coverage of superficial second-degree burns is significantly lower in AM than in SD group (P < .001) and also those of deep second-degree burns (P < .001). Application was painless in AM than SD group (P < .001). State of pain in-between application shows significant difference (P < .001). Application of AM was comfortable to the attending doctor (P < .001). Significant activity of the patients was observed during treatment (P < .01) with AM. AM was accepted by the patients or parents (P < .001). This study indicates that radiation-sterilized, oven-dried AM is a better treatment option because its use reduces hospital stay and the number of dressing changes. Epithelialization of the wound is quicker. The use of AM is painless and odorless. The procedure is easy and comfortable to the doctor, and it is well accepted. Most of the patients remain ambulatory during treatment.

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Section: Discussionmentioning

confidence: 99%

Comparisons of the Effects of Biological Membrane (Amnion) and Silver Sulfadiazine in the Management of Burn Wounds in Children

Mostaque

Rahman²

2011

Journal of Burn Care & Research

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“…High doses of irradiation up to 50 kGy do not have significant effect on the biomechanical properties of bone [25,65] . However, in most of the reports, the decrease of maximum load of cortical bone was observed after gamma irradiation with doses over 30 kGy [63,66,74,75] . Gamma irradiation has adverse effect on the mechanical and biological properties of bone allografts due to degradation of collagen in the bone matrix.…”

Section: Effect Of Gamma Radiation On Biomechanical Properties Of Tismentioning

confidence: 99%

“…Scanning electron microscopy (SEM) for collagen structure [62] , infrared spectroscopy for chemical structure of amniotic membrane [63] , bone graft models for osteoinduction and bone absorption [64] , and compression or bending tests for mechanical properties have been used [26,[65][66][67] . Voggenreiter et al [62] studied the bone surface structure of cortical bone grafts using SEM and observed no deleterious effects of cryopreservation and irradiation.…”

Section: Properties Of Tissuesmentioning

confidence: 99%

Radiation sterilization of tissue allografts: A review

Singh

2016

WJR

Self Cite

167

137

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Tissue substitutes are required in a number of clinical conditions for treatment of injured and diseased tissues. Tissues like bone, skin, amniotic membrane and soft tissues obtained from human donor can be used for repair or reconstruction of the injured part of the body. Allograft tissues from human donor provide an excellent alternative to autografts. However, major concern with the use of allografts is the risk of infectious disease transmission. Therefore, tissue allografts should be sterilized to make them safe for clinical use. Gamma radiation has several advantages and is the most suitable method for sterilization of biological tissues. This review summarizes the use of gamma irradiation technology as an effective method for sterilization of biological tissues and ensuring safety of tissue allografts.

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“…H&E staining was performed to confirm removing the amniotic epithelium. Then, the basement membrane can be preserved by drying or glycerol -cryopreservation after γsterilization [3,4,5].…”

Section: Preparationmentioning

confidence: 99%