Effect of High Calorie Diet on Intestinal Flora in LPS-Induced Pneumonia Rats

Abstract

Intestinal flora plays an important role in inflammatory response to systemic or local organs of its host. High calorie diet has been shown to aggravate the condition of pneumonia and delay recovery, especially in children. However, the underlying mechanisms remain unclear. This study placed SPF rats in a conventional environment, high calorie diet or LPS atomization was performed respectively or combined. Analysis of high-throughput sequencing of intestinal content combined with animal weight, organ index, serum inflammatory factors indicators and bioinformatics found that after pulmonary infection combined with a high-calorie diet, rats showed significant changes such as weight loss and increased lung weight index, and their lung and intestinal tissues showed more obvious inflammatory changes. And its gut flora structure suggests, the abundance of Leuconostocaceae in significantly reduced; abundance of Staphylococcus, Planococcaceae, Staphylococcus, Staphylococcaceae, Bacillales, Gemellales and Aerococcus significant increased. The study showed that high calorie diet and LPS atomization synergistically promoted pneumonia process in rat pups, which is related to changes in structure of intestinal flora. It is worth noting that pneumonia rats fed by convention diet also causing intestinal flora imbalance. Long-term high calorie diets have a negative regulatory effect on health. Studies have shown that 1 high calorie diet may aggravate the condition of pneumonia and delay recovery in respiratory infectious diseases. Changes in the rhythm of modern life caused changes in structure of intestinal flora 2 , such as dietary imbalances 3,4. It is suggested that changes in dietary composition may cause changes in internal ecosystem of intestine 5. The healing or aggravation of respiratory inflammation is also related to structure of intestinal flora. For example, the hypothesis theory of "pulmonary-gut axis" 6 indicates that local inflammatory reactive substances of respiratory system and products of intestinal flora metabolism exchanged and stimulated through lymphatic system and blood circulation system. However, it is still unclear whether intake of high calorie diet aggravates inflammatory response of respiratory tract influenced by intestinal flora. This study explored potential influence of high calorie diet on aggravating pneumonia rats in intestinal flora. Materials and Methods Animals and reagents. Animals: Clean grade SD rats, 40, male, 4 weeks old, weighing 110 g ± 10 g. Animal certificate No.: 11401500016361, provided by Sibeifu (Beijing) Biotechnology Co., Ltd., license No.: SCXK (Jing) 2016-0002. It is kept in animal laboratory of Institution of Traditional Chinese Medicine, Beijing University of Chinese Medicine with free access to food and water, natural light. All animal experiments in this study were carried out in accordance with the relevant guidelines and regulations and approved by the Animal Ethics Review Committee of Beijing University of Traditional Chinese Medicine, with the approval number (Ani...

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