Effect of HD-03, a herbal formulation, on the antioxidant defence system in rats

Abstract

The effect of HD‐03, a herbal formulation was investigated on the hepatocellular antioxidant defence system in CCl4 treated rats. Three doses of CCl4 were administered orally with liquid paraffin (1:1) to induce hepatic damage. Twenty four hours after the last dose, blood was collected by decapitation for the estimation of serum ALT and AST. The levels of antioxidant enzymes, lipid peroxidation and glycogen in the liver were estimated. Nineteen days pretreatment with HD‐03 (750 mg/kg) significantly reversed CCl4‐induced changes in serum ALT and AST levels. HD‐03 pretreatment also significantly reversed the CCl4‐induced changes in the different components of the cellular antioxidant system and lipid peroxidation. Pretreatment with HD‐03 significantly restored the hepatic glycogen levels which were depleted in CCl4 intoxicated rats. The observed reversal produced by HD‐03 in the serum AST and ALT may be due to the membrane stabilizing potential which helps in preventing the leakage of intracellular enzymes into the systemic circulation. The increase in the hepatic glycogen levels in HD‐03 pretreated rats, indicates its preventive effect on subcellular injury caused by CCl4, which leads to glycogenolysis, as a result of disturbance in the intracellular Ca+2 pool. The inhibition of lipid peroxidation and enhancement in the activity of antioxidant enzymes by HD‐03 may be due to the direct free radical scavenging activity and reactivation of these enzymes in the liver. Thus the antioxidant potential and inhibitory effect on­lipid peroxidation may play an important role in the antihepatotoxic activity of HD‐03. © 1998 John Wiley & Sons, Ltd.

Hepatoprotective Property of Schumannianthus Virgatus (Roxb.)rolfe Against D-Galactosamine Induced Hepatotoxicity in Wistar Rats.

“…Galactosamine administration in rats disrupts the membrane permeability of the plasma membrane, causing leakage of the enzymes from the cell, which leads to elevation in levels of serum enzymes (Mitra et al, 2000). It is found from this study that the levels of AST, ALT, ALP, GGT, total cholesterol, bilirubin and triglycerides increased significantly in the group treated with D-GalN compared to normal control and it indicates hepatic damage.Results are shown in Table:1.…”

Hepatoprotective and some haematological parameters effect of Allium ampeloprasum against carbon tetrachloride induced liver toxicity in albino rats

“…These vitamins and minerals work together to help stabilize blood . Calcium in leeks is also used for the proper clotting of blood in the human body in addition of iron was increased the haemopoiesis process in the bone marrow due to significant increased levels (p<0.05) of the hemoglobin and packed cell volume in the blood (36). Allium species also have immune enhancing actions that include promotion of lymphocyte synthesis, cytokine release, phagocytes and natural killer-cell activity (37) .…”

Hepatoprotective activity of Trapa acornis shell extracts against CCl4-induced liver injury in rats

“…According to the results, the high total phenolic and total flavonoid of TASEA and TASBU suggested their potential uses as a potential source of antioxidants in food applications. Hepatoprotective studies showed that plants have active ingredients that are capable of free radical scavenging in living systems (Mitra et al, 1998). In this study, the antioxidant activity of TAS was assayed by estimation of MDA and SOD levels in vivo.…”