Effect of Growth Regulators on Direct Clonal Propagation and Analysis of Total Phenolic Content of Wild and Propagated Mucuna pruriens

Abstract

Mucuna pruriens is an important vegetable cover crop. Almost every part of the plant is thoughtto contain L-3,4 dihydroxyphenylalanine (L-Dopa). The present study describes a quick anddependable technique for high-constancy miniature spread. Helper nodal explants were refinedon Murashige and Skoog’s (MS) media boosted with different groupings of cytokinins from15-day-old refined explants. Throughout the primary culture on 35 to 45 mg/l 6-benzyl aminopurine (BAP) greatest no of shoots was delivered. The number of shoots expanded when theflawless shoots were exposed to re-refine on a similar hormonal medium. The stretched shootscreated a limit of roots on half-strength MS medium enhanced with 20-40 mg/l naphthaleneacidic corrosive (NAA). The Plantlets were adjusted by moving them first to peat greenery:manure (1:1) combination further with sand: soil (1:1) blend, recording 90% endurance. Thisframework gives high constancy miniature engendering framework for proficient and fastminiature proliferation and evaluation of phenolic content of this significant green compostcover crop with restorative properties.