Effect of glycerol on intracellular virus survival: implications for the clinical use of glycerol-preserved cadaver skin

The Development and Characterization of an Organotypic Tissue-Engineered Human Esophageal Mucosal Model

“…The tissue was washed in PBS and the epithelium peeled off using forceps and discarded. The remaining tissue was washed x3 in PBS and placed into 80% glycerol solution for 24 hours, transferred to 90% glycerol for 24 hours and stored in 100% glycerol at room temperature for a minimum of 4 months in order to sterilise the tissue; previous research having shown that this was a reliable method for the storage and sterilisation of skin whilst preserving tissue integrity 21 . When required the tissue was re-hydrated by multiple washing in PBS and sterility tested by incubating in DMEM at 37 °C.…”

Quality Sample Collection, Handling, and Preservation for an Effective Microbial Forensics Program

“…This medium is not used in the United States and has not been thoroughly tested for PCR inhibition. Glycerol, at different concentrations, may inactivate certain viruses while preserving others in clinical samples (25,41,42). A possible criterion for selecting an adequate collection and transport medium for viruses of agricultural concern could be the use of isotonic solutions with neutral pH (23).…”

Flow Cytometric Viability Assessment and Transmission Electron Microscopic Morphological Study of Bacteria in Glycerol

“…Glycerol has the advantages of preservation of the skin structure, reduction of tissue antigenicity, and antimicrobial~Van Baare et al, 1998! andantiviral~Van Baare et al, 1994;Marshall et al, 1995! properties. Although some articles have been published concerning a potential antimicrobial effect of 85% glycerol, some controversies persist concerning this effect~Kearney, 1998; Lomas et al, 2004!…”