Effect of Fiber Architecture on the Cell Functions of Electrospun Fiber Membranes

Sultana, Fariha; Vaughan, Melville B.; Khandaker, Morshed

doi:10.1007/978-3-319-41351-8_22

Cited by 4 publications

(6 citation statements)

References 3 publications

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“…Higher magnification confocal microscopy images confirm ( Figure 8 ) that the spheroid like cells are attaching onto PCL material using cytoplasmic extensions and more attachment can be seen at the PCL junctions. We have observed a similar trend of increased attachment of osteoblast cells at the PCL junctions on glass slides during the study of the effect of the number of PCL fiber layers on cell adhesion and proliferation on PCL membranes [ 22 ].…”

Section: Resultsmentioning

confidence: 80%

“…In addition, we are performing cytocompatibility analyses of PEGDA-PCL with varying number of layers (12 layers vs. 24 layers) for 7, 14 and 21 days using adult human mesenchymal stem cells. We have used osteoblast cells to understand the effect of architecture of PCL fiber layers on cell viability on PCL membranes [ 22 ]. Another limitation of the study is the use of the hepatocellular carcinoma cells in the scaffold instead of using primary liver or skin (fibroblasts) cells.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications

Kotturi

Abu-Abed

Zafar

et al. 2017

JFB

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Polyethylene Glycol Diacrylate (PEGDA) tissue scaffolds having a thickness higher than 1 mm and without the presence of nutrient conduit networks were shown to have limited applications in tissue engineering due to the inability of cells to adhere and migrate within the scaffold. The PEGDA scaffold has been coated with polycaprolactone (PCL) electrospun nanofiber (ENF) membrane on both sides to overcome these limitations, thereby creating a functional PEGDA-PCL scaffold. This study examined the physical, mechanical, and biological properties of the PEGDA and PEGDA-PCL scaffolds to determine the effect of PCL coating on PEGDA. The physical characterization of PEGDA-PCL samples demonstrated the effectiveness of combining PCL with a PEGDA scaffold to expand its applications in tissue engineering. This study also found a significant improvement of elasticity of PEGDA due to the addition of PCL layers. This study shows that PEGDA-PCL scaffolds absorb nutrients with time and can provide an ideal environment for the survival of cells. Furthermore, cell viability tests indicate that the cell adhered, proliferated, and migrated in the PEGDA-PCL scaffold. Therefore, PCL ENF coating has a positive influence on PEGDA scaffold.

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Section: Resultsmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications

Kotturi

Abu-Abed

Zafar

et al. 2017

JFB

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show abstract

“…Kotturi et al [ 36 ] used 12 layers of PCL NFM to produce PEGDA-PCL samples, whereas this study used 24 layers of PCL NFM. This study used a higher number of fiber layers because our previous studies observed a correlated higher amount of cellular attachment and proliferation for PCL membrane with a higher number of fiber layers [ 45 ]. Kotturi et al [ 36 ] studied how human hepatocellular carcinoma cells (GS5 cells) adhered, proliferated, and migrated higher in the PEGDA-PCL scaffold than in PEGDA alone.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Polycaprolactone Electrospun Nanofiber-Composites for Artificial Skin Based on Dermal Fibroblast Culture

et al. 2022

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The study’s aim was to develop a dermal equivalent scaffold that can mimic the architecture and biological performance of the human dermis. Poly ε-caprolactone (PCL) electrospun nanofiber material (ENF) was assembled with polyethylene glycol diacrylate (PEGDA), sodium alginate (SA) and type I collagen (CG1) to develop three groups of dermal equivalent scaffolds. These scaffolds were named PEGDA-PCL, SA-PCL and CG1-PCL. Scanning electron microscopy (SEM) images of cell-free scaffolds’ top and cross-sectional surface were collected and analyzed to examine internal morphology, specifically the adhesiveness of PCL fibers with the different scaffolds. Human dermal fibroblasts were cultured on each of the scaffolds. Cell viability studies including cell adhesion, cell differentiation and stress fiber production were conducted on each scaffold. Furthermore, the architectural integrity of each scaffold was verified by degradation analysis for 2 weeks by soaking each scaffold in phosphate-buffered saline (PBS) solution. Finally, we conducted rheological characteristics of each scaffold. Based on our results from the above analysis, the study concluded that CG1-PCL is best suitable for the dermal equivalent model and has potential to be used as a graft for skin repair.

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“…This study produced PCL NFM using an electrospun process which was presented in our earlier publication [16]. In short, PCL beads and acetone were sonicated for 45 min, and then the solution was poured into a 12 mL plastic syringe.…”

Section: Sample Preparationmentioning

confidence: 99%

Glutathione Immobilized Polycaprolactone Nanofiber Mesh as a Dermal Drug Delivery Mechanism for Wound Healing in a Diabetic Patient

et al. 2022

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Glutathione (GSH) is an anti-inflammatory and antioxidant biomolecule. Polycaprolactone (PCL) nanofiber mesh (NFM) is capable of the attachment and release of biomolecules for prolonged periods and has the potential as a transdermal drug delivery system during wound healing for a diabetic patient. Our earlier study found that high levels of sugar in diabetic male mice were significantly decreased by daily doses of glutathione administered on the mice. Furthermore, oxidative stress found in diabetic male mice led to the total depletion of glutathione levels in the body’s organs (pancreas, spleen, epididymis, and testis). The objective of this study was to attach GSH with PCL NFM for the controlled release of GSH biomolecules for long periods of time from the fiber mesh into a diabetic body. This study produced PCL NFM using an electrospun technique and tested it on mice to evaluate its efficiency as a dermal drug delivery mechanism. This study dissolved GSH (2.5 mg/mL) with phosphate-buffered saline (PBS) and glutaraldehyde (GLU) solution to create GSH-PBS and GSH-GLU complexes. Each complex was used to soak PCL NFM for 24 h and dried to create PCL-GSH-PBS and PCL-GSH-GLU meshes. Fiber morphology, degradation, fibroblast cell proliferation, cytotoxicity, and GSH release activities from each mesh were compared. Fibroblast cell adhesion and cytotoxicity tests found excellent biocompatibility of both GSH-immobilized PCL meshes and no degradation until 20 days of the study period. The disk diffusion method was conducted to test the antibacterial properties of the sample groups. Release tests confirmed that the attachment of GSH with PCL by GSH-GLU complex resulted in a steady release of GSH compared to the fast release of GSH from PCL-GSH-PBS mesh. The disk diffusion test confirmed that PCL-GSH-GLU has antibacterial properties. The above results conclude that GSH-GLU immobilized PCL NFM can be a suitable candidate for a transdermal anti-oxidative and anti-bacterial drug delivery system such as bandage, skin graft for wound healing application in a diabetic patient.

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Effect of Fiber Architecture on the Cell Functions of Electrospun Fiber Membranes

Cited by 4 publications

References 3 publications

Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications

Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications

Evaluation of Polycaprolactone Electrospun Nanofiber-Composites for Artificial Skin Based on Dermal Fibroblast Culture

Glutathione Immobilized Polycaprolactone Nanofiber Mesh as a Dermal Drug Delivery Mechanism for Wound Healing in a Diabetic Patient

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