EFFECT OF EXOGENOUS PROLINE IN TWO SUGARCANE GENOTYPES GROWN in vitro UNDER SALT STRESS

Medeiros, Maria das Graças Freire de; Silva, Marina Medeiros de Araújo; Granja, Manuela Maria Cavalcante; Silva-Junior, Gilberto de Souza e; Câmara, Terezinha Rangel; Willadino, Lília

doi:10.15446/abc.v20n2.42830

Cited by 14 publications

(13 citation statements)

References 29 publications

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“…To cope with ROS, plants provides enzymatic (SOD, CAT, GPX and APX) and non-enzymatic antioxidant defense that helps to reduce oxidative damage and improve plant tolerance and resistance to drought and salt stress [55,56].Treatment with antioxidants, especially the combined antioxidants and proline led to synergistic effects between them and caused enhancement in enzymatic antioxidants in chickpea plants. Similar results have been reported by Medeiros et al [57], who found that there was an increase in APX activity in sugarcane using 20 mM proline. Furthermore, ASA foliar application increased CAT, SOD and Calendula POX activity [58].…”

Section: Changes In Enzymatic Antioxidantssupporting

confidence: 91%

Role of Ascorbic acid, Glutathione and Proline Applied as Singly or in Sequence Combination in Improving Chickpea Plant through Physiological Change and Antioxidant Defense under Different Levels of Irrigation Intervals

2020

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In recent years, the harmful effects of drought stress have been be mitigated by using bioactive compounds such as antioxidants and osmolytes. In this research, pot experiments were carried out to investigate the effects of ascorbic acid, glutathione and proline on alleviating the harmful effect of drought stress in chickpea plants during season 2017. Chickpea plant seeds were soaked in ascorbic acid (0.75 mM), glutathione (0.75 mM), proline (0.75 mM) singly and/or in sequence combinations for 4 h and then planted in pots. The pots were irrigated with water after seven days (to serve as control), after 14 days (moderate drought stress) and after 28 days (severe drought stress). The sequence combination of antioxidants and proline under drought stress has not been studied yet. The results showed significantly decreased in plant growth, yielding characteristics, photosynthetic pigments and soluble protein content in response to moderate and severe drought stress. Moreover, treatment with antioxidants caused increment the antioxidant enzyme activity, non-enzymatic antioxidant (ascorbic acid and glutathione) contents and endogenous proline in stressed and unstressed plants. In conclusion, The sequence combination of antioxidants and proline caused improvement in plant growth under drought stress by up-regulating the antioxidant defense system and osmolyte synthesis.

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Section: Changes In Enzymatic Antioxidantssupporting

confidence: 91%

Role of Ascorbic acid, Glutathione and Proline Applied as Singly or in Sequence Combination in Improving Chickpea Plant through Physiological Change and Antioxidant Defense under Different Levels of Irrigation Intervals

2020

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“…The outcome of increased proline concentration on saline stress has been extensively studied in plants, such as sorghum (De Lacerda et al, 2003), rice (Lima et al, 2004) and canola (Saadia et al, 2012). Specifically on sugar cane, when subjected to 100 mM of NaCl, it is observed the accumulation of this osmolyte in leaves and roots (García and Medina, 2003;Vasantha and Rajlakshmi, 2009;Karpe et al, 2012;Medeiros et al, 2015) which corroborates the present work. This accumulation is observed in salt tolerant sugarcane cultivars, because this is a mechanism of protection against salt stress.…”

Section: Discussionsupporting

confidence: 88%

“…Willadino et al (2011) and Medeiros et al (2015) observed in different sugar cane varieties a constant behavior of the enzyme up to 100 mM of NaCl, after that concentration there was an increase of the enzyme's activity, which was not observed in the present study, this may have occurred due to differences in tolerance between the varieties used. APX is another enzyme that actively participates in the removal of H 2 O 2 by transforming the hydrogen peroxide into water through the oxidation of ascorbate (Teixeira et al, 2004).…”

Section: Discussioncontrasting

confidence: 75%

Response to in vitro salt stress in sugarcane is conditioned by concentration and condition of exposure to NaCl

Granja¹,

Medeiros²,

Silva³

et al. 2018

Acta biol. Colomb.

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Salinity is one of the major environmental stress factors that affect crop productivity, as well as interfering with plant growth and development, resulting in reduced production quality. Given this, we highlight the importance of research in response to plants subjected to salt stress in order to assess the physiological and biochemical behavior of genotypes, with the objective of selecting the more tolerant. One way to ensure the uniformity of the response of the plants is through in vitro cultivation, which allows control of the cultivation conditions. Therefore, the objective of this work was to evaluate the response of two commercial varieties of sugar cane exposed to saline stress in different conditions (gradual and abrupt). Two varieties of sugarcane (RB931011 and RB872552) were subjected to in vitro salt stress by NaCl (56 mM and 112 mM), either gradually or suddenly. The responses of the enzymatic antioxidant system (catalase, peroxidases and ascorbate peroxidase) and free proline, as well as the Na + and K + contents, were assessed 30 days after the beginning of the treatments. Differences were observed in the responses of the varieties as a function of the induction mode of salt stress, graded or by shock, rather than as a function of the NaCl concentrations in the culture medium. The stress response is therefore conditioned not only by salt concentration, but also by the form of exposure to salt.Keywords: antioxidant enzymes, in vitro culture, oxidative stress, salinity. RESUMENLa salinidad es uno de los principales factores de estrés ambiental, además de interferir en el crecimiento de las plantas perjudica directamente la producción agrícola. En ese contexto, se destaca la importancia de investigaciones direccionadas a la respuesta de las plantas sometidas al estrés salino, con el fin de evaluar el comportamiento fisiológico y bioquímico con el objetivo de seleccionar genotipos tolerantes a dicha condición. Una de las técnicas más utilizadas para uniformizar la respuesta de las plantas a una condición en particular es el cultivo de tejidos in vitro. Por lo tanto, el objetivo de este estudio fue evaluar la respuesta de dos variedades comerciales de caña de azúcar (RB931011 e RB872552) expuestas a estrés salino con NaCl (56 mM e 112 mM) en diferentes condiciones, gradual y abrupta. Las respuestas del sistema antioxidante enzimático (catalasa, peroxidasa y ascorbato peroxidasa) y prolina libre, asi como las concentraciones de Na + e K + fueron evaluadas 30 días después del inicio de los tratamientos. Fueron observadas diferencias en la respuesta de las variedades en función del modo de inducción del estrés salino, graduado o abrupto, y no solo en función de las concentraciones de NaCl en el medio de cultivo. La respuesta al estrés es condicionada no solo por la concentración de sal sino también por la forma de exposición al medio salino.Palabras clave: cultivo in vitro, enzimas antioxidantes, estrés oxidativo, salinidad.

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“…Penggunaan prolin untuk memperkuat toleransi tanaman tebu terhadap kekeringan dapat dilakukan dengan pendekatan molekular (transgenik) atau aplikasi eksogen prolin pada tanaman atau perlakuan benih. Perbedaan genotipe tanaman tebu berpengaruh pada akumulasi prolin indigenous (Medeiros et al 2015). Tanaman toleran kekeringan mampu mengakumulasi prolin lebih banyak dibanding yang peka.…”

Section: Prolinunclassified

Respon Fisiologis Tanaman Tebu Terhadap Kekeringan, Physiological Responses of Sugarcane Plant to Drought

Mastur¹

2017

Bul. Tan. Tembakau, Serat & Minyak Industri

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ABSTRAKProduksi tebu nasional sering terhambat oleh adanya masalah kekeringan. Tujuan dari penulisan tinjauan ini adalah untuk membahas hasil-hasil penelitian tentang respon fisiologi tanaman tebu terhadap kekeringan sebagai dasar bagi pengelolaannya baik secara genetik maupun teknologi budi daya. Melalui tinjauan ini diharapkan kegiatan perakitan varietas unggul toleran kekeringan dan teknologi budi daya untuk penurunan dampak kekeringan lebih efektif. Kekeringan menyebabkan perubahan aktivitas fisiologis penting dimulai dari penutupan stomata untuk menekan transpirasi, penurunan input karbondioksida, penurunan jumlah klorofil, dan pada akhirnya penurunan laju fotosintesis netto. Akar merespon kekeringan dengan mensintesis dan mengirimkan sinyal asam absisat (ABA) yang mengakibatkan penutupan stomata sehingga menurunkan transpirasi dan serapan CO2. Kekeringan menyebabkan penurunan kandungan klorofil a, b, dan nisbah klorofil a/b. Penurunan laju fotosintesis dan kegiatan fisiologis lain menurunkan pertumbuhan tanaman tebu, dan akhirnya produktivitas gula. Genotipe yang toleran kekeringan menunjukkan kemampuan untuk meminimalkan pengaruh buruk kekeringan. Tingkat kerugian dari kekeringan paling besar pada fase perpanjangan, karena fase kebutuhan air yang besar untuk meningkatkan bobot tebu, terutama untuk pemanjangan batang. Fase pemasakan membutuhkan air paling sedikit, namun sangat penting karena menentukan rendemen gula. Konsentrasi senyawa osmoprotektan, yang membantu mengatasi peningkatan potensial osmosis sel, pada genotipe tanaman toleran kekeringan meningkat tinggi pada kondisi kekeringan. Senyawa osmoprotektan dapat berupa kelompok asam amino, gula atau amonium kuarter, seperti prolin, trehalosa, dan glisin betain. Senyawa lain untuk ketahanan kekeringan adalah larutan kompatibel yang memiliki bobot molekul rendah, mudah larut dan non-toksik dalam sitosol. Pemahaman biosintesis dan fungsi senyawa tersebut merupakan dasar dari upaya pemanfaatannya untuk penelitian dan pengelolaan kekeringan terutama melalui pemuliaan bioteknologi, konvensional, maupun produksi senyawa osmoprotek-tan untuk aplikasi eksogen.Kata kunci: Saccharum officinarum, fotosintesis, kekeringan, osmoprotektan ABSTRACT National sugarcane production is often inhibited by drought problem. The objective of this review is to discuss research findings on physiological responses of sugarcane to drought as a base for genetic and cultivation management. Through this review drought tolerance breeding activities and cultivation technology for mitigation impact will be coped with more effectively. Drought influenceses important physiological activities from stomata closure to minimize transpiration, reducing carbon dioxide input, chlorophyll and nett photosynthesis. Plant roots respond to drought througout biosynthesis and send signal to abscisic acid (ABA) for closing stomata to reduce transpiration and CO2 absorption. Drought reduces chlorophyll a, b, and a/b ratio. Reducing photosynthesis rate and other physiological activities inhibit sugarcan...

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EFFECT OF EXOGENOUS PROLINE IN TWO SUGARCANE GENOTYPES GROWN in vitro UNDER SALT STRESS

Cited by 14 publications

References 29 publications

Role of Ascorbic acid, Glutathione and Proline Applied as Singly or in Sequence Combination in Improving Chickpea Plant through Physiological Change and Antioxidant Defense under Different Levels of Irrigation Intervals

Role of Ascorbic acid, Glutathione and Proline Applied as Singly or in Sequence Combination in Improving Chickpea Plant through Physiological Change and Antioxidant Defense under Different Levels of Irrigation Intervals

Response to in vitro salt stress in sugarcane is conditioned by concentration and condition of exposure to NaCl

Respon Fisiologis Tanaman Tebu Terhadap Kekeringan, Physiological Responses of Sugarcane Plant to Drought

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