Effect of exercise to exhaustion on myeloperoxidase and lysozyme release from blood neutrophils

Abstract

Exercise sessions (swimming in rats and treadmill running in humans) resulted in stimulation of neutrophil degranulation in the experiments with animals and in the human study. Myeloperoxidase (MPO) (+67%) and lysozyme (+51%) quantities in the plasma of rats increased significantly immediately after exercise. The blood plasma lysozyme concentration was increased by 41% at the 6th min of treadmill exercise in athletes. The blood concentrations of neutrophil proteins normalized both in humans and animals at rest. The neutrophil protein concentrations in blood increased in parallel with the decrease of their level in leukocytes. The neutrophil capacity for an oxidative burst was not changed by the exercise, but decreased for 3-6 h in the post-exercise period. Such dynamics of the oxidative burst activity suggest a lack of association between this parameter and the degranulation process. The neutrophil proteins that appear in blood during degranulation can be involved in enhancing the bactericidal potency of blood, the activation of granulopoiesis, neutrophil efflux from bone marrow, and the conditioning of blood endothelium for leukocyte extravasation.

Antioxidant supplementation influences the neutrophil tocopherol associated protein expression, but not the inflammatory response to exercise

“…Intense exercise induces the degranulation of neutrophils, which leads to an increase of the plasma concentration of marker neutrophil enzymes (elastase and MPO) [8] The increase in circulating neutrophils, and plasma MPO concentration after the half marathon, reflect neutrophil mobilization and priming for oxidative activity due to intense exercise. The demargination and degranulation stimulus might be related to the action of hormones such as catecholamines or glucocorticoids [37,38].…”

“…Physical activity induces neutrophil priming for oxidative burst and activates acute phase protein release [7,8]. An increase in MPO and lysozyme plasma concentrations has been evidenced as a result of exercise [8,9]. Exhaustive exercise also increases the neutrophil generation of reactive oxygen species (ROS) and lipid peroxidation and induces oxidative stress [10].…”

Innate immune responses to a single session of sprint interval training

“…Neutrophil OBA was expressed in 2 ways: 1) as the stimulated-area under curve, in which the integral of the stimulated chemiluminescence curve was calculated and the integral of the unstimulated control curve (chemiluminescence response without stimulation) was subtracted; 2) as the stimulated-peak chemiluninescnece (peak stimulated minus unstimulated chemiluninescnece at the equivalent time). It was assumed that the chemiluminescence response depends almost entirely on neutrophils (Morozov et al 2003) therefore the chemiluminescence responses were divided by the number of neutrophils per well in order to express OBA per neutrophil.…”

Effects of bovine colostrum supplementation on upper respiratory illness in active males

“…Following addition of fMLP, area under the curve (over 300 s), above the 180 stable unstimulated state was used to determine stimulated CL. To calculate 181 responses on a per cell basis, it was assumed that the stimulated CL is entirely from 182 neutrophils (Morozov et al, 2003). Thus, fMLP-stimulated area under the CL curve 183 was divided by the number of neutrophils present in each well to give CL in RLU (i.e.…”