Effect of Er:YAG and Diode Laser Irradiation on the Root Surface: Morphological and Thermal Analysis

Theodoro, Letícia Helena; Haypek, Patrícia; Bachmann, Luciano; Garcia, Valdir Gouveia; Sampaio, José Jackson Coelho; Zezéll, Denise Maria; Eduardo, Carlos

doi:10.1902/jop.2003.74.6.838

Cited by 229 publications

(99 citation statements)

References 30 publications

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“…The temperature rise of teeth placed in a 378C water bath after laser irradiation was reported to be 58C lower than the temperature rise of isolated teeth [34]. Nonetheless, the measurement of temperature rise is widely used as a convenient and fundamental method in evaluating the thermal effect of laser on pulp tissue [35][36][37], as the precisely controlled laboratory conditions can hardly be conducted in vivo.…”

Section: Discussionmentioning

confidence: 99%

Bactericidal effects of diode laser on Streptococcus mutans after irradiation through different thickness of dentin

et al. 2006

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Background and Objectives: A reliable method to eradicate the bacteria of residual carious dentin has not yet been developed. The aim of this study was to evaluate the antibacterial effect of a diode laser on Streptococcus mutans through different thickness (500, 1,000, and 2,000 mm) of human dentin. The thermal effect of laser irradiation was also investigated. Study Design/Materials and Methods: Dentin specimens were inoculated with 2 ml of S. mutans on one side and irradiated by a diode laser on the other side with a power output ranging from 0.5 to 7 W. The laser tip was swept with the whole irradiation area of 7 mmÂ3 mm at a speed of about 10 mm/second with a total irradiation time of 30 seconds. Cooling with distilled water (30 ml/minute) was applied simultaneously during laser irradiation. After laser irradiation, the bacteria was removed from the dentin surfaces and cultured for 48 hours at 378C anaerobically to assess the colony forming units (CFU) per ml. The morphology of the lased bacteria and the temperature rise during laser irradiation were observed by scanning electron microscope (SEM) and measured by thermocouple, respectively. Results: The results revealed that 7 W of laser power could kill 97.7% of CFU through 500 mm thickness of dentin. However, the bactericidal efficiency was significantly reduced as the dentin thickness was increased. The morphological changes of lased bacteria ranged from less affected such as loss of their wall bands and existence of minicells to more severely degenerated, such as disintegration and fusion of cells with pores on the cell wall. Only the dentin specimens with a thickness of 500 mm exhibited a temperature rise greater than 5.58C after receiving 5 or 7 W of laser irradiation. Conclusions: A diode laser can eliminate the Streptococcus mutans of the residual carious dentin without inducing high pulpal temperature rise when the remaining dentin thickness is greater than 1 mm.

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Section: Discussionmentioning

confidence: 99%

Bactericidal effects of diode laser on Streptococcus mutans after irradiation through different thickness of dentin

et al. 2006

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“…3,4) Recently, the clinical use of lasers has increased, and lasers are now employed frequently even for endodontic treatment. 5,6) In particular, short wavelength Nd:YAG lasers and Ga-Al-As lasers are used for coagulation of deep tissue in the body, as well as for surgical treatment, hard tissue formation, and root canal therapy. [7][8][9] Against this background, we have been studying the anti-inflammatory effect of GaAl-As laser irradiation when used as an adjuvant to accelerate hard tissue formation.…”

mentioning

confidence: 99%

Stimulatory Effects of Hydroxyl Radical Generation by Ga-Al-As Laser Irradiation on Mineralization Ability of Human Dental Pulp Cells

Matsui

Tsujimoto

Matsushima

2007

Biological & Pharmaceutical Bulletin

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The present study was conducted to investigate the effects of Ga-Al-As laser irradiation on the mineralization ability of human dental pulp (HDP) cells. HDP cells in vitro were irradiated once with a Ga-AL-As laser at 0.5 W for 500 s and at 1.0 W for 500 s in order to investigate free radicals as one mechanism for transmission of laser photochemical energy to cells. Production of the hydroxyl radical ( · OH) was measured using the ESR spintrapping method and was found to be increased by laser irradiation. The DMPO-OH was not detected in the presence of dimethyl sulfoxide (DMSO), a · OH scavenger. The formation of calcification nodule was also investigated by von Kossa staining. The number of calcified nodules was increased by 1.0 W-laser irradiation. Alkaline phosphatase (ALP) activity was higher in the 1.0 W-laser irradiation group. Expression of mRNAs for heat shock protein 27, bone morphogenetic proteins (BMPs) and ALP were greater in the 1.0 W-laser irradiation group. Expression of BMPs in the conditioned medium was also higher in the 1.0 W-laser irradiation group. In particular, DMSO decreased the number of calcified nodule produced by 1.0 W-laser irradiation. These results supposed that the mineralization of HDP cells is stimulated by laser irradiation, and that · OH generated by laser irradiation is a trigger for promotion of HDP cell mineralization.

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“…[49][50][51] The study by Theodoro et al showed that applying laser for 30 seconds with water spray can decrease temperature (-2.2 ± 2.5°C). 52 It should be taken into consideration that using too much water or air cooling results in energy absorption and decreased ablation effect of laser. 53 Another parameter that affects the antibacterial effect of laser, is radiation time.…”

Section: Anti-bacterial Effect Of Er:yag Laser On Enterococcus Faecalismentioning

confidence: 99%

The Effect of Er:YAG Laser on Entroccocus faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth

et al. 2017

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Introduction: Successful root canal therapy depends on the complete elimination of microorganisms such as Entroccocus faecalis, which is impossible to achieve with the traditional methods. Lasers are recently introduced as a new method to solve the problem. The present study is planned and performed to examining the antibacterial effect of Er: YAG laser. Methods: Sixty extracted anterior primary teeth were prepared and sterilized. E. faecalis bacterium was cultured in canals. Samples were randomly divided into two groups. The first group was disinfected by NaOCl 5/25% and Er: YAG laser and the second group just by NaOCl 5/25%. Samples of canal contents were cultured and colony counts were calculated. The results were analyzed statistically by SPSS software and Mann Whitney test. Results: There was no significant difference between colony counts in both groups (P = 0.142). But the number of colonies in the first group was lower than in the second group. Conclusion: Although, Er: YAG laser cannot completely eliminate E. faecalis bacterium, its simultaneous use with NaOCl decreases E. faecalis.

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Effect of Er:YAG and Diode Laser Irradiation on the Root Surface: Morphological and Thermal Analysis

Abstract: The application of Er:YAG and diode lasers at the utilized parameters did not induce high pulpal temperatures. Root surface irregularities were more pronounced after irradiation with an Er:YAG laser than with a diode laser.

Cited by 229 publications

References 30 publications

Bactericidal effects of diode laser on Streptococcus mutans after irradiation through different thickness of dentin

Bactericidal effects of diode laser on Streptococcus mutans after irradiation through different thickness of dentin

Stimulatory Effects of Hydroxyl Radical Generation by Ga-Al-As Laser Irradiation on Mineralization Ability of Human Dental Pulp Cells

The Effect of Er:YAG Laser on Entroccocus faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth

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