Effect of enamel matrix derivative liquid in combination with a natural bone mineral on new bone formation in a rabbit GBR model

Abstract

Objectives:Recently, a new liquid carrier system for enamel matrix derivate (EMDliquid) was developed with better physico-chemical properties for improved adsorption of EMD to biomaterial surfaces. The aim of the present study was to investigate the bone regenerative potential of EMD-liquid in combination with a natural bone mineral (NBM) in vivo.Methods: Four 6-mm defects were created in the calvaria of six New Zealand white rabbits. Defects were filled with either (1) control (empty), (2) 20 mg of NBM, or (3) 20 mg of NBM + 20 µl of EMD-liquid (n = 8). All defects were covered with collagen barrier membranes. The bone regenerative potential was investigated by micro-CT and histomorphological analyses at 8 weeks postsurgery. Results:The mineralized tissue volume was significantly higher in the NBM + EMDliquid group when compared to control, whereas no difference was observed between the NBM alone and control groups. While no significant difference was observed for horizontal bone defect closure between the NBM + EMD-liquid and the NBM alone groups, NBM + EMD-liquid significantly increased the total mineralized area and reduced the percentage of soft/connective tissue infiltration. No statistically significant difference was observed between the NBM + EMD-liquid group and NBM alone group for amount of mineralized bone. Conclusion:The addition of EMD-liquid did not lead to statistically significant bone formation when compared to NBM alone. The combination of NBM + EMD-liquid but not NBM alone did however induce superior mineralized tissues when compared to control (empty). Further research investigating the adsorption potential of EMDliquid to bone-grafting particles with/without collagen may provide valuable insights into future regenerative strategies with enamel matrix proteins. K E Y W O R D Sbone formation, EMD, enamel matrix derivative, GBR, guided bone regeneration, Osteogain | 543 KOBAYASHI et Al.

Effect of enamel matrix derivative on alveolar ridge preservation in the posterior maxilla: A randomized controlled clinical trial

“…In an in vitro study, Miron et al 33 found that EMD in a liquid type carrier with bone grafting materials showed higher absorption of amelogenin than the gel type counterpart if used with bone grafting materials. Several pre‐clinical studies evaluating bone formation capability of EMD in a liquid type carrier showed significantly greater new bone formation and less soft/connective tissue infiltration 32‐34 . These variations in EMD carrier may result in improvement of efficacy of EMD for the ARP procedure, but further research, including in vivo and clinical studies are required.…”

Polymeric biomaterials for periodontal tissue engineering and periodontitis

“…A statistically significant increase in mineralized tissue was observed in white rabbits treated with NBM + EMD-liquid. 72 According to the results of a study published in 2019 by Lee et al it was also observed that the use of EMD in patients undergoing periodontal procedures greatly reduces the amount of pain and swelling felt and patients have a more comfortable post-operation period. 73 In addition to GFs that have been researched, there are GFs such as epidermal growth factor (EGF), insulin-like growth factor (IGF), stromal cell derived factor-1 (SDF-1), which have been less researched but have a positive effect on periodontal tissue regeneration.…”

“…86,87 Retrovirus, lentivirus, adenovirus, vaccinia virus, and adeno-associated virus (AAV) are examples of viral vectors. [88][89][90][91][92] Non-viral vectors have naked DNA which makes them easier to manufacture at low costs and insert into the cells. Gene delivery via non-viral vectors is called transfection.…”

The Applications of Enamel Matrix Derivative in Implant Dentistry: A Narrative Review

“…Since the effects of amelogenins on cells involved in periodontal regeneration found substantial support in the literature, the hypothesis that EMD could stimulate osteoblast and bone regeneration has been studied both in recently published animal [ 8 , 9 ] and human studies [ 10 , 11 ]. Jung et al [ 9 ] compared the effect of EMD-liquid as an adjunct to biphasic calcium phosphate (BCP) in a rabbit calvarial model, as evaluated through micro-CT and histomorphometric analysis.…”