Effect of Electrospun Scaffold Fiber Alignment on Endothelial Cell Growth

Hom, Sarah; O'Hara, Joseph; Rubenstein, David A.

doi:10.1096/fasebj.29.1_supplement.792.2

Cited by 3 publications

(2 citation statements)

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“…Electrospinning has been used to design highly porous scaffolds that mimic topological cues of the ECM within native arteries. − Nano/microfiber scaffolds with different degrees of fiber- alignment and -diameter have been developed to optimize structural and mechanical properties of VGs in an effort to regulate cell adhesion, shape, spreading, and migration. − …”

Section: Introductionmentioning

confidence: 99%

Combining Electrospun Fiber Mats and Bioactive Coatings for Vascular Graft Prostheses

et al. 2016

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The patency of small-diameter (<6 mm) synthetic vascular grafts (VGs) is still limited by the absence of a confluent, blood flow-resistant monolayer of endothelial cells (ECs) on the lumen and of vascular smooth muscle cell (VSMC) growth into the media layer. In this research, electrospinning has been combined with bioactive coatings based on chondroitin sulfate (CS) to create scaffolds that possess optimal morphological and bioactive properties for subsequent cell seeding. We fabricated random and aligned electrospun poly(ethylene terephthalate), ePET, mats with small pores (3.2 ± 0.5 or 3.9 ± 0.3 μm) and then investigated the effects of topography and bioactive coatings on EC adhesion, growth, and resistance to shear stress. Bioactive coatings were found to dominate the cell behavior, which enabled creation of a near-confluent EC monolayer that resisted physiological shear-flow conditions. CS is particularly interesting since it prevents platelet adhesion, a key issue to avoid blood clot formation in case of an incomplete EC monolayer or partial cell detachment. Regarding the media layer, circumferentially oriented nanofibers with larger pores (6.3 ± 0.5 μm) allowed growth, survival, and inward penetration of VSMCs, especially when the CS was further coated with tethered, oriented epithelial growth factor (EGF). In summary, the techniques developed here can lead to adequate scaffolds for the luminal and media layers of small-diameter synthetic VGs.

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Section: Introductionmentioning

confidence: 99%

Combining Electrospun Fiber Mats and Bioactive Coatings for Vascular Graft Prostheses

et al. 2016

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“…A morfologia de scaffolds vasculares também é uma propriedade importante a ser avaliada. Diversos estudos mostram que fatores como área superficial, dimensões, porosidade, rugosidade e orientação de fibras (alinhada e desalinhada) influenciam na adesão, proliferação e diferenciação celular (HOM et al, 2015;CHEN, H. L. et al, 2017;RODRIGUES et al, 2020). Além disso, estruturas que possuem propriedades similares a MEC possibilitam a melhor mimetização dos vasos sanguíneos (JORDAN et al, 2016).…”

Section: Avaliação Das Propriedades Do Scaffold Para Engenharia De Te...unclassified

Desenvolvimento de scaffold polimérico para aplicações na engenharia de tecidos vasculares

Caroline Pereira Rodrigues

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À Deus por toda proteção e todas as bençãos que recebi em minha vida.Aos meus pais, Vinícius e Débora, que me ensinaram a ser uma pessoa íntegra, a acreditar em mim e lutar pelos meus sonhos, e que me fazem querer ser melhor a cada dia. Por todo amor incondicional e por serem luz que me guia de onde estiverem.À toda minha família, que sempre torce por mim. Às minhas avós, Luzia e Jamila, por todo amor, traduzido em cada pequeno gesto. Aos meus tios, principalmente Alessandra, Rodrigo, Cyntia e Mikio, que me apoiaram e aconselharam em muitos momentos. Aos meus irmãos, Guilherme e Alexandre, que sempre me dão força e coragem quando preciso. Ao Alex por todo apoio, amizade, paciência e amor. Por todas as ligações diárias, essenciais para recarregar minha energia, todos os momentos que aprendemos e crescemos juntos e toda reciprocidade. À Luciana, Alex, Nicolas e Igor por me acolherem como parte de sua família e por todo carinho. Aos meus amigos que deixaram comigo um pouco de si para eu carregar aonde for. Meus amigos de infância e ensino médio, que mesmo com o tempo e distância não se deixaram afastar. Aos amigos da faculdade, principalmente Ana e Pracuch, que sempre me incentivaram e se fizeram presentes. E aos meus amigos de Limeira que, com sua companhia, tornaram essa cidade tão especial para mim. À minha orientadora, Laís, por todo conhecimento, experiência, ajuda e amizade ao longo do mestrado. Por ter confiado em mim desde o início e ter me ajudado a tornar a pesquisadora que sou hoje. Aos membros da banca, titulares e suplentes, por se disponibilizarem a enriquecer meu projeto, na qualificação e defesa da minha dissertação, com todo seu conhecimento e experiência.

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Fabrication of random and aligned-oriented cellulose acetate nanofibers containing betamethasone sodium phosphate: structural and cell biocompatibility evaluations

Saifoori

Fallah-Darrehchi

Zahedi

et al. 2017

Journal of Polymer Engineering

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Abstract: The objective of this work was to prepare electrospun cellulose acetate (CA) nanofibers containing betamethasone sodium phosphate (BSP). Two different morphologies including random and aligned orientations were rationally designed to improve the performance of samples in in vitro experiments. By comparing the CA nanofibrous samples with randomly and aligned-oriented morphologies, the scanning electron microscopy images showed that the neat aligned-oriented nanofibers with an average diameter of 180±15 nm could be obtained using a high-speed rotating collector. Subsequently, the tensile test confirmed that the aligned CA nanofibers had higher mechanical properties than that of the randomly oriented ones. Moreover, the BSP release profile obtained by UV-vis spectrophotometry depicted that the aligned samples had an initial burst release of BSP followed by a slow penetration of the drug with a gentle slope during 72 h. Furthermore, the ultimate amounts of BSP released from the random and aligned CA nanofibers into the phosphate buffer solution were 63% and 53%, respectively. Finally, human adipose-derived mesenchymal stem cells were seeded on both aligned and random electrospun CA nanofibrous samples containing BSP. The thiazolyl blue and hematoxylin and eosin staining results showed that the BSP-loaded nanofibers with the aligned morphology provided the most suitable environment for the cells’ growth, viability, and proliferation.

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Effect of Electrospun Scaffold Fiber Alignment on Endothelial Cell Growth

Cited by 3 publications

References 0 publications

Combining Electrospun Fiber Mats and Bioactive Coatings for Vascular Graft Prostheses

Combining Electrospun Fiber Mats and Bioactive Coatings for Vascular Graft Prostheses

Desenvolvimento de scaffold polimérico para aplicações na engenharia de tecidos vasculares

Fabrication of random and aligned-oriented cellulose acetate nanofibers containing betamethasone sodium phosphate: structural and cell biocompatibility evaluations

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