Effect of donor age on cultivation of human oral mucosal keratinocytes

In vitro cell culture system optimization of keratinocytes from oral lichen planus (OLP) patients

“…As previously reported, donor age is an important factor that significantly affects the culture of normal oral keratinocytes in vitro (Lauer et al , ; Grossman et al , ). Normal oral keratinocytes from older donors demonstrate weaker proliferative ability and longer incubation time to reach confluence.…”

Use of oral mucosal cell sheets for accelerated oral surgical wound healing

“…In these studies, the plating efficiency of primary disaggregated skin cells was around 1% and increased to 5% for newborn donors . However, CFEs of epithelial cells in oral mucosa were higher than those of skin or limbus, depending on donor age . In plating efficiency experiments with the first passage (P1), the CFEs of primary cultured oral keratinocytes were up to 33%.…”

“…4 However, CFEs of epithelial cells in oral mucosa were higher than those of skin or limbus, 12 depending on donor age. 15 In plating efficiency experiments with the first passage (P1), the CFEs of primary cultured oral keratinocytes were up to 33%. Accordingly, CFEs were reportedly high in cells of later passages with fewer holoclone cells.…”

Tissue engineering of cultured skin substitutes

“…-the growth of multilayered epithelial transplants (so called "sheet grafts") [13,14,37,44,46,53,57,59,61,93,110,118,120,121,141,145]; -the construction of composite multi-layered dermalepidermal analogues [13, 18, 20, 24, 25, 30, 37, 41-43, 48, 52, 53, 59, 61, 63, 78, 81, 84, 110, 130, 133, 135-137, 143, 144, 147, 149]; -and the concept of preconfluent cell grafting [13, 19, 23, 29, 39, 47, 49, 50, 58-63, 67, 68, 76, 82-85, 87, 100, 126, 134, 141, 143, 145]. A summary of the most well known techniques to produce and apply cultured skin substitutes and most of the currently available methods and research directions can be found in Table 3.…”