Effect of Different Doses of Vitamin E with Selenium on Renal Damage Caused by Unilateral Ureteral Obstruction Model?

Abstract

Objective: Chronic kidney disease (CKD) is still common worldwide. We investigated the effect of two different doses of vitamin E with selenium (Se) administration to prevent CKD leads to renal damage via unilateral ureteral obstruction (UUO).Material and Methods: Thirty-two female Wistar Albino rats were divided into four groups; a sham group (left UUO+no medication); left UUO with no treatment, left UUO treated with 100 mg/kg dose Vitamin E+Se (RPVE+Se) and left UUO treated with 1000 mg/kg Vitamin E+Se (HDVE+Se) mixture. All rats subjected during 14 days and killed humanely. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), levels were determined in kidney tissue, whereas total antioxidant status (TAS), total oxidant status (TOS) and DNA damage marker 8-hydroxy-2-deoxyguanosine (8-OHdG) in serum. Histologic examination were also done. Results:The unilateral ureteral obstruction model increased MDA (p<0.05), TOS (p<0.001) and 8-OHdG levels (p<0.001) via oxidative mechanisms. In the treatment groups increased TAS, SOD (p< 0.001), and GSH (p<0.001) levels were determined, but in RPVE+Se group, TAS levels were slightly higher than the HDVE+Se group (p=0,039). In terms of histological findings, tubular necrosis (p=0.003) and lymphocyte infiltration (p=0.002) were observed renal tissue images. RPVE+Se group findings were similar to the sham group, while in HDVE group these images similar to the UUO group. Conclusions:It was observed that UUO caused oxidative stress resulting in renal damage, and controlled vitamin E application with Selenium administration, which is considered to be an effective antioxidant couple, inhibited oxidative stress.