Effect of Different Combinations of the Growth Factors and Hormones on In vitro Maturation of Goat Preantral Follicles

Shankaraiah, P.; Swathi, B.; Kumari, G. Aruna; Priyanka, B.; Prasad, C. S.; Amin, Ruhul

doi:10.20546/ijcmas.2018.704.224

Cited by 1 publication

(1 citation statement)

References 14 publications

(24 reference statements)

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“…The factors secreted by the cumulus cells regulate the disruption of the gap junction and cumulus expansion in pig oocytes [51]. Other studies reported that oocytes obtained in medium supplemented with growth hormone (GH), IGF-I, EGF, thyroxin (T4), and FSH had the maximum percentage of M-II stage oocytes (38.6%) when compared to the other treatments, revealing that in vitro development of goat PFs could be significantly improved through addition of GH, EGF, T4, and FSH in the best combination [52]. However, differential rates of maturation of cattle oocytes in vitro were found upon supplementation of FBS (81.7% ± 12.9%) and estrous calf serum (85.7 ± 12.7%) in TCM-199 containing 5 µg/mL FSH and 1 µg/mL estradiol [53].…”

Section: Ivm In 25 Ng/ml Leptin With Different Combinations Of Igf-i Egf Fbs and Bsamentioning

confidence: 96%

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Talukdar¹,

Biswas²,

Kadirvel³

et al. 2020

Turk J Vet Anim Sci

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The present study aimed to evaluate the effect of supplementing epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), fetal bovine serum (FBS), and bovine serum albumin (BSA) in different combinations in a maturation medium (MM) comprising TCM-199 + 5 µg/mL 17-β estradiol + 5 µg/mL ovine-luteinizing hormone (oLH) + 5 µg/mL pure follicle-stimulating hormone (pFSH) + 100 µM/mL cysteamine containing the best concentration of leptin among 10/20/25/30 ng/mL on in vitro maturation (IVM) and subsequent vitrification of matured goat oocytes. The IVM of oocytes was carried out in a CO 2 incubator for 27 h at 38.5 °C. The percentage of oocytes with cumulus expansion and polar body extrusion was found to be highest in 25 ng/mL leptin at 47.37% and 15.63%; rates of 66.67% and 28.57% were obtained when supplemented with 50 ng/mL IGF-I + 10 ng/mL EGF in medium containing 25 ng/mL leptin as compared to the other media. The in vitro matured oocytes were vitrified in 5 M ethylene glycol + 5 M propylene glycol with 0.5 M sucrose in TCM-199 + 20% FBS using liquid nitrogen. The highest percentage of morphologically intact in vitro matured oocytes following vitrification was obtained in medium containing 25 ng/mL leptin (89.29%). It was concluded that addition of IGF-I + EGF with 25 ng/mL leptin in MM yielded a higher rate of maturation of oocytes, and supplementation of 25 ng/mL leptin in MM resulted in a higher rate of morphologically intact vitrified oocytes, indicating the efficiency of leptin for cryotolerance.

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Section: Ivm In 25 Ng/ml Leptin With Different Combinations Of Igf-i Egf Fbs and Bsamentioning

confidence: 96%

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Talukdar¹,

Biswas²,

Kadirvel³

et al. 2020

Turk J Vet Anim Sci

View full text Add to dashboard Cite

show abstract

Effect of Different Combinations of the Growth Factors and Hormones on In vitro Maturation of Goat Preantral Follicles

Cited by 1 publication

References 14 publications

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

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