Effect of Different Carbon Sources on Bacterial Nanocellulose Production and Structure Using the Low pH Resistant Strain Komagataeibacter Medellinensis

Abstract

Bacterial cellulose (BC) is a polymer obtained by fermentation with microorganism of different genera. Recently, new producer species have been discovered, which require identification of the most important variables affecting cellulose production. In this work, the influence of different carbon sources in BC production by a novel low pH-resistant strain Komagataeibacter medellinensis was established. The Hestrin-Schramm culture medium was used as a reference and was compared to other media comprising glucose, fructose, and sucrose, used as carbon sources at three concentrations (1, 2, and 3% w/v). The BC yield and dynamics of carbon consumption were determined at given fermentation times during cellulose production. While the carbon source did not influence the BC structural characteristics, different production levels were determined: glucose > sucrose > fructose. These results highlight considerations to improve BC industrial production and to establish the BC property space for applications in different fields.

Statistical optimization of culture conditions to improve cell density ofKomagataeibacter medellinensisNBRC 3288: the first step towards to optimize bacterial cellulose production

“…In Figure 1 at 72 h starts deceleration of cell growth that is in line to results in static conditions obtained by Molina-Ramírez et al (Molina-Ramírez et al 2017) and agitated conditions obtained by Yoshinaga, Tonouchi, Watanabe (Yoshinaga et al 1997) and Okiyama, Shirae, Kano, Yamanaka (Okiyama et al 1992) with an strain belong to Komagataeibacter genus. These results suggest that fermentation mode (static or agitated) did not affect growth profile, but agitated mode allows obtain a higher cell density yield compared to static mode, in agitated mode a biomass measured by was 1.091(OD 600 ) against 0.690 (OD 600 ) obtained in static fermentation mode (data not shown).…”

“…These results suggest that fermentation mode (static or agitated) did not affect growth profile, but agitated mode allows obtain a higher cell density yield compared to static mode, in agitated mode a biomass measured by was 1.091(OD 600 ) against 0.690 (OD 600 ) obtained in static fermentation mode (data not shown). As was mentioned above, 72 h is the age where the K. medellinensis NBRC 3288 begins deceleration stage and match with begins of BC production according to Molina-Ramírez et al (Molina-Ramírez et al 2017), these results suggest that an inoculum of 72 h, would be the most appropriate to use in the further BC production process (Chao et al 2000; Bae and Shoda 2004). Likewise, Table 2 shows the results of maximum growth rate (μ m , h −1 ) in each treatment evaluated and as it was expected, the highest mm was obtained in the treatment with the highest biomass, which granted that culture medium of treatment 7 is the best to improve cell density yield of K. medellinensis NBRC 3288.…”

Development of novel three‐dimensional scaffolds based on bacterial nanocellulose for tissue engineering and regenerative medicine: Effect of processing methods, pore size, and surface area

“…The diffraction peaks were fitted using pseudo‐Voigt and Gaussian functions. The crystallinity index (Cr.I) was calculated according to Molina‐Ramirez et al as the percentage of the area of all crystalline peaks to the total area . The d‐spacing (d) between the crystal planes was determined using Bragg's law expressed by Eq.…”

Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions

“…D-Glucose, ethanol, D-fructose, maltose, sucrose, sorbitol, D-mannitol, D-ribose and D-xylose were reported as suitable carbon sources for this strain [26]. The cellulose producing ability of this strain has been assessed on different occasions [22,37,38]. However, reports on BNC production and the optimal conditions were not consistent, possibly due to the differing fermentation conditions, thus we have carried out the optimization study, using reported parameters as a starting point.…”