Effect of dietary polyphenols on fructose uptake by human intestinal epithelial (Caco-2) cells

Andrade, Nicole Pamplona Bueno de; Araújo, João Pedro; Correia-Branco, Ana; Carletti, Jaqueline Vieira; Martel, Fátima

doi:10.1016/j.jff.2017.07.032

Cited by 40 publications

(23 citation statements)

References 52 publications

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“…a, the TEER values for AP‐BL and BL‐AP increased steadily during the first 10 days, rapidly increased from days 10 to 12 and remained stable at approximately 400–500 Ω·cm 2 on subsequent days. It was reported that TEER values in the range from 200 to 1000 Ω·cm 2 suggested good tight junctions of Caco‐2 monolayers; the higher TEER value resulted in more tight junctions of the cell monolayers (Andrade et al ., ). The TEER values between days 19 and 21 days of incubation were in the range from 450 to 500 Ω·cm 2 , which met with the experimental requirements.…”

Section: Resultsmentioning

confidence: 97%

Antioxidant activity in HepG2 cells, immunomodulatory effects in RAW 264.7 cells and absorption characteristics in Caco‐2 cells of the peptide fraction isolated from Dendrobium aphyllum

Liu

Sun

et al. 2018

Int J of Food Sci Tech

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Summary DA‐P, fraction of peptides with a molecular weight <1 kDa isolated from Dendrobium aphyllum, was analysed in three types of cell lines to verify its bioactivity and absorptivity. The cellular antioxidant activity of DA‐P in HepG2 cells was used and results revealed an EC50 of 2.88 ± 0.143 mg mL−1 and a CAA unit of 63.46 ± 2.11 μm QE/100 g peptides. DA‐P treatment enhanced the secretion of cytokines in RAW 264.7 cells. After demonstrating the presence of tight junctions in Caco‐2 monolayers, the absorption was 25.57% ± 0.016% and 19.7% ± 0.012% from different sides. The relatively high absorption indicated that the antioxidant‐relevant immune functions of DA‐P had a greater possibility to be absorbed by Caco‐2 cells. Free amino acids and LC‐MS/MS analysis indicated the degradation and expulsion of components after the absorption of DA‐P, and Ser‐Ser‐Arg was able to come across the monolayers.

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Section: Resultsmentioning

confidence: 97%

Antioxidant activity in HepG2 cells, immunomodulatory effects in RAW 264.7 cells and absorption characteristics in Caco‐2 cells of the peptide fraction isolated from Dendrobium aphyllum

Liu

Sun

et al. 2018

Int J of Food Sci Tech

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“…PPO is an important enzyme that has numerous health-promoting properties including antiviral, anticarcinogen, antimicrobial, and antimutagenic agents [37][38][39] and can be used in several industrial applications [40,41]. However, this enzyme is also known to be responsible for the enzymatic browning of fruits [42].…”

Section: Polyphenoloxidase (Ppo) Activity Of Fresh and Dried Mangomentioning

confidence: 99%

Effect of Heat Exposure on Activity Degradation of Enzymes in Mango Varieties Sindri, SB Chaunsa, and Tommy Atkins during Drying

2020

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Mango has been described as a valuable source of nutrients and enzymes that are beneficial to human health. Drying at different temperatures not only affects the nutritional properties but can also contribute to the degradation of valuable enzymes in dried fruit. The novelty of this paper is to investigate the quality of hot air dried mango in terms of activity retention of the heat-sensitive enzymes (HSE). For this, HSE was first screened in fresh mango flesh of the variety Samar Bahisht (SB) Chaunsa. Later, the combined effect of different drying temperatures (40 °C, 50 °C, 60 °C, 70 °C, and 80 °C) and air velocities (1.0 ms−1 and 1.4 ms−1) on the activity retention of HSE in dried mango slices of the varieties Sindri, SB Chaunsa, and Tommy Atkins were investigated. The results showed that the drying temperature had a significant impact on the degradation of HSE, while at the same time some influence of the air velocity was also observed. Drying at 40 °C and an air velocity of 1.4 ms−1 retained more HSE compared to those samples dried at higher temperatures. The least retention of HSE was found in samples dried at 80 °C.

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“…To further investigate the possible internalization mechanism, the cellular uptake of ginsenoside liposomes was inhibited by various GLUT inhibitors, including D-glucose (blocking GLUT1), phloridzin (blocking SGLT1), and quercetin (blocking GLUT2 and GLUT5) 29, 35, 41, 42. In the competition assay, at least one inhibitor exhibited significant inhibition of the internalization of each type of ginsenoside liposomes in the BGC-823 cells (Figure 2D-F, Figure S1A-C); therefore, the main endocytic pathway of ginsenoside liposomes was GLUT carrier-mediated.…”

Section: Resultsmentioning

confidence: 99%

Novel ginsenoside-based multifunctional liposomal delivery system for combination therapy of gastric cancer

et al. 2019

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The clinical treatment of gastric cancer (GC) is hampered by the development of anticancer drug resistance and the unfavorable pharmacokinetics, off-target toxicity, and inadequate intratumoral accumulation of the current chemotherapy treatments. Ginsenosides combined with paclitaxel (PTX) have been shown to exert synergistic inhibition of human GC cell proliferation. In the present study, we developed a novel multifunctional liposome system, in which ginsenosides functioned as the chemotherapy adjuvant and membrane stabilizer. These had long blood circulation times and active targeting abilities, thus creating multifunctionality of the liposomes and facilitating drug administration to the GC cells. Methods: Three ginsenosides with different structures were used to formulate the unique nanocarrier, which was prepared using the thin-film hydration method. The stability of the ginsenoside liposomes was determined by particle size analysis using dynamic light scattering. The long circulation time of ginsenoside liposomes was compared with that of conventional liposome and polyethylene glycosylated liposomes in vivo . The active targeting effect of ginsenoside liposomes was examined with a GC xenograft model using an in vivo imaging system. To examine the antitumor activity of ginsenoside liposomes against GC, MTT, cell cycle, and apoptosis assays were performed on BGC-823 cells in vitro and PTX-loaded ginsenoside liposomes were prepared to evaluate the therapeutic efficacy on GC in vivo . Results: The ginsenosides stabilized the liposomes in a manner similar to cholesterol. We confirmed the successful delivery of the bioactive combination drugs and internalization into GC cells via analysis of the glucose-related transporter recognition and longer blood circulation time. PTX was encapsulated in different liposomal formulations for use as a combination therapy, in which ginsenosides were found to exert their inherent anticancer activity, as well as act synergistically with PTX. The combination therapy using these targeted liposomes significantly suppressed GC tumor growth and outperformed most reported PTX formulations, including Lipusu ® and Abraxane ® . Conclusion: We established novel ginsenoside-based liposomes as a tumor-targeting therapy, in which ginsenoside functioned not only as a chemotherapy adjuvant, but also as a functional membrane material. Ginsenoside-based liposomes offer a novel platform for anticancer drug delivery and may lead to a new era of nanocarrier treatments for cancer.

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Effect of dietary polyphenols on fructose uptake by human intestinal epithelial (Caco-2) cells

Cited by 40 publications

References 52 publications

Antioxidant activity in HepG2 cells, immunomodulatory effects in RAW 264.7 cells and absorption characteristics in Caco‐2 cells of the peptide fraction isolated from Dendrobium aphyllum

Antioxidant activity in HepG2 cells, immunomodulatory effects in RAW 264.7 cells and absorption characteristics in Caco‐2 cells of the peptide fraction isolated from Dendrobium aphyllum

Effect of Heat Exposure on Activity Degradation of Enzymes in Mango Varieties Sindri, SB Chaunsa, and Tommy Atkins during Drying

Novel ginsenoside-based multifunctional liposomal delivery system for combination therapy of gastric cancer

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