Effect of dietary intake of phenolic compounds from mango peel extract on growth, lipid peroxidation and antioxidant enzyme activities in zebrafish (Danio rerio)

Abstract

Four experimental diets were formulated with 50, 100, 150 and 200 mg of phenolic compounds (PCs) kg-1 feed, derived from mango peel. The control diet did not contain PCs. A total of 120 male zebrafish (Danio rerio) (average weight: 166 mg) were fed for eight weeks to assess the ability of PCs to prevent lipid peroxidation and enhance antioxidant status. Growth performance was calculated at the end of the experimental trial. Lipid peroxidations in muscle and antioxidant enzyme activity in the liver were evaluated at the end of the experiment. There was no significant difference in growth performance among treatments. Malondialdehyde (MDA) levels in muscle were significantly lower in fish fed diets containing 50 and 100 mg of PCs per kg of feed. Incorporation of PCs into zebrafish diet did not have any significant effects on glutathione peroxidase and superoxide dismutase activity. However, catalase (CAT) activity increased significantly in fish with diets containing 100, 150 and 200 mg of PCs kg-1 feed. These results suggest a potential protective effect against in vivo lipid peroxidation and CAT-modulating effects.

Corn Husk Phenolics Modulate Hepatic Antioxidant Response in Nile Tilapia (Oreochromis niloticus) Exposed to Hypoxia

“…•and H 2 O 2 triggers oxidative stress, decreasing cellular antioxidant defenses [14], which causes oxidative liver cell damage because the liver is the main metabolizing and detoxifying organ [12]. Previous studies have reported that hypoxia-induced oxidative stress inhibits the first-line antioxidant defense, made up of CAT, SOD, and GPx in the liver of freshwater fish [9,14].…”

“…In this regard, research has focused on finding low-cost natural antioxidants that reduce or prevent cell damage caused by hypoxia-induced oxidative stress. Plant wastes are a rich source of phenolic compounds (PCs) widely used as antioxidant additives in aquafeeds to enhance the hepatic antioxidant response and prevent its oxidative damage in fish [6,12,13]. PCs exert their antioxidant effect through the electrons/protons donation that neutralizes ROS or modulates the SOD, CAT, and GPx activities [13,14].…”

Polyphenolic Extracts from Spent Coffee Grounds Prevent H2O2-Induced Oxidative Stress in Centropomus viridis Brain Cells

“…This assay was performed according to Lizárraga-Velázquez et al [58] and Solé et al [59] with slight modifications. Briefly, 50 µL of the supernatant were mixed with 163 µL of 1 methyl-2-phenylindole (10.3 mM, in methanol:acetonitrile, 1:3; v/v), 50 µL of distilled water and 75 µL of HCl at 37%.…”

Antioxidant Molecules from Plant Waste: Extraction Techniques and Biological Properties

“…For instance, phenolic compounds from avocado seed and peel have been demonstrated to possess antioxidant properties by showing high radical scavenging activity against ROS, such as peroxyl superoxide (ROO) (2.2-10.6 µmol TE/g) and hypochlorous acid (HOCl) (EC 50 = 5.2-8.6 µg/mL) [173]. Furthermore, phenolic compounds from mango peels, such as gallic acid and quercetin, increase the activity of CAT and reduce the MDA levels, as a biomarker of lipid peroxidation, in an in-vivo model of zebrafish (Danio rerio) [174]. Lycopene (4.9%), a tetraterpene, from tomato peel, has been demonstrated to exert antioxidant activity via reducing the levels of ROS and diminishing the DNA fragmentation induced by H 2 O 2 in L6 cells (myoblasts) [175].…”