Effect of dietary docosahexaenoic acid on rhodopsin content and packing in photoreceptor cell membranes

Senapati, Subhadip; Gragg, Megan; Samuels, Ivy S.; Parmar, Vipul M.; Maeda, Akiko; Park, Paul Shin-Hyun

doi:10.1016/j.bbamem.2018.03.030

Cited by 34 publications

(44 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another important conclusion from our study is that the consequences of DHA deficit caused by the Mfsd2a knock-out are different from those caused by dietary DHA restriction. Dietary restriction in mice and rats could lead to a higher degree of DHA deprivation of the retina associated with reductions in ERG responses, outer segment shortening and alteration in the rate of photoreceptor outer segment renewal without notable photoreceptor loss (Benolken et al, 1973;Wheeler et al, 1975;Senapati et al, 2018). However, this diet leads to a major substitution of DHA with docosapentaenoic acid (22:5; Anderson et al, 1974;Niu et al, 2004), which does not happen in Mfsd2a Ϫ/Ϫ retinas.…”

Section: Discussionmentioning

confidence: 99%

“…It is generally agreed that, like the brain, the retina cannot synthesize its own DHA de novo and relies on uptake from extraretinal sources, such as blood-borne lipids, for maintaining its DHA content (Scott and Bazan, 1989;Bazan et al, 2011). Multiple studies analyzing the consequences of DHA dietary restriction documented that DHA-deprived retinas produced light responses of reduced sensitivity (Benolken et al, 1973;Wheeler et al, 1975;Senapati et al, 2018), suggesting that DHA may be critical for supporting visual signaling. Recently, Silver and colleagues showed that Mfsd2a is expressed in retinal pigment epithelium (RPE) and retinal vasculature and that the Mfsd2a-mediated lipid transport in RPE is particularly important for maintaining the high DHA concentration in the retina (Wong et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Disrupted Blood-Retina Lysophosphatidylcholine Transport Impairs Photoreceptor Health But Not Visual Signal Transduction

et al. 2019

View full text Add to dashboard Cite

Retinal photoreceptor cells contain the highest concentration of docosahexaenoic acid (DHA) in our bodies, and it has been long assumed that this is critical for supporting normal vision. Indeed, early studies using DHA dietary restriction documented reduced light sensitivity by DHA-deprived retinas. Recently, it has been demonstrated that a major route of DHA entry in the retina is the delivery across the blood-retina barrier by the sodium-dependent lipid transporter, Mfsd2a. This discovery opened a unique opportunity to analyze photoreceptor health and function in DHA-deprived retinas using the Mfsd2a knockout mouse as animal model. Our lipidome analyses of Mfsd2a ؊/؊ retinas and outer segment membranes corroborated the previously reported decrease in the fraction of DHA-containing phospholipids and a compensatory increase in phospholipids containing arachidonic acid. We also revealed an increase in the retinal content of monounsaturated fatty acids and a reduction in very long chain fatty acids. These changes could be explained by a combination of reduced DHA supply to the retina and a concomitant upregulation of several fatty acid desaturases controlled by sterol regulatory element-binding transcription factors, which are upregulated in Mfsd2a ؊/؊ retinas. Mfsd2a ؊/؊ retinas undergo slow progressive degeneration, with ϳ30% of photoreceptor cells lost by the age of 6 months. Despite this pathology, the ultrastructure Mfsd2a ؊/؊ photoreceptors and their ability to produce light responses were essentially normal. These data demonstrate that, whereas maintaining the lysophosphatidylcholine route of DHA supply to the retina is essential for long-term photoreceptor survival, it is not important for supporting normal phototransduction.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Disrupted Blood-Retina Lysophosphatidylcholine Transport Impairs Photoreceptor Health But Not Visual Signal Transduction

et al. 2019

View full text Add to dashboard Cite

show abstract

“…First, a member of the phospholipid flippase family has been shown to be essential for odourant receptor responses in Drosophila melanogaster 44,45 , suggesting that the PL composition of the OSN plasma membrane is an important factor for olfactory signalling. A number of studies also demonstrated that DHA-rich retinal PLs enhance the activity of rhodopsin, a receptor that belongs to the same G-protein-coupled receptor class as odourant receptors [46][47][48][49][50][51][52][53] . Specific interactions between DHA and rhodopsin improve the stability of the receptor 54 .…”

Section: Con L Owmentioning

confidence: 99%

Perinatal exposure to diets with different n-6:n-3 fatty acid ratios affects olfactory tissue fatty acid composition

Khoury

Soubeyre

Cabaret

et al. 2020

Sci Rep

View full text Add to dashboard Cite

The olfactory mucosa (OM) and the olfactory bulb (OB) are responsible for the detection and processing of olfactory signals. Like the brain and retina, they contain high levels of n-3 and n-6 polyunsaturated fatty acids (PUFAs), which are essential for the structure and function of neuronal and non-neuronal cells. Since the influence of the maternal diet on olfactory lipid profiles of the offspring has been poorly explored, we examined the effects of feeding mice during the perinatal period with diets containing an adequate linoleic acid level but either deficient in α-linolenic acid (ALA) or supplemented in n-3 long-chain PUFAs on the lipid composition of dams and weaning offspring olfactory tissues. In both the OM and OB, the low n-3 ALA diet led to a marked reduction in n-3 PUFAs with a concomitant increase in n-6 PUFAs, whereas consumption of the high n-3 PUFA diet reduced n-6 PUFAs and increased n-3 PUFAs. Structural analysis showed that the molecular species profiles of the main phospholipid classes of olfactory tissues from weaning pups were markedly affected by the maternal diets. This study demonstrates that the PUFA status of olfactory tissues is sensitive to diet composition from the early stages of development.

show abstract

“…OS is heavily loaded with a large number of closed membranous sacs, which overlap as pancakes stacked in a perpendicular array respect to the major axis of the rod. Rhodopsin and others GPs visual pigments move along and within (flip-flop) the phospholipid bilayer thus being their functionality fully dependent of the viscosity/fluidity balance of the sacs membranes, fact which in turns is mainly determined for the relative ratio of ω3/ω6 /ω9 PUFAs and cholesterol [81, 82]. Rods are distributed by the retina together with the cones but are better adapted for vision with little light intensity or twilight vision (scotopic vision), which does not allow a proper the perception of colors.…”

Section: Introductionmentioning

confidence: 99%

“…1 and 3). DHA and other ω3 and ω6 LCHU-PUFAs are separated and esterified in triglycerides, stored in the abundant oil droplets of PEC and then re-uptaked by myoid area of rod and cones [8, 12, 82, 103, 104].…”

Section: Introductionmentioning

confidence: 99%

Role of ω3 polyunsaturated fatty acids in diabetic retinopathy: a morphological and metabolically cross talk among blood retina barriers damage, autoimmunity and chronic inflammation

Eynard

Repossi

2019

Lipids Health Dis

View full text Add to dashboard Cite

Vision disorders are one of the most serious complications of diabetes mellitus (DM) affecting the quality of life of patients and eventually cause blindness. The ocular lesions in diabetes mellitus are located mainly in the blood vessels and retina layers. Different retina lesions could be grouped under the umbrella term of diabetic retinopathies (DMRP). We propose that one of the main causes in the etiopathogenesis of the DMRP consists of a progressive loss of the selective permeability of blood retinal barriers (BRB). The loss of selective permeability of blood retinal barriers will cause a progressive autoimmune process. Prolonged autoimmune injures in the retinal territory will triggers and maintains a low-grade chronic inflammation process, microvascular alterations, glial proliferation and subsequent fibrosis and worse, progressive apoptosis of the photoreceptor neurons. Patients with long-standing DM disturbances in retinal BRBs suffer of alterations in the enzymatic pathways of polyunsaturated fatty acids (PUFAs), increase release of free radicals and pro-inflammatory molecules and subsequently incremented levels of vascular endothelial growth factor. These facts can produce retinal edema and photoreceptor apoptosis. Experimental, clinical and epidemiological evidences showing that adequate metabolic and alimentary controls and constant practices of healthy life may avoid, retard or make less severe the appearance of DMRP. Considering the high demand for PUFAs ω3 by photoreceptor complexes of the retina, it seems advisable to take fish oil supplements (2 g per day). The cellular, subcellular and molecular basis of the propositions exposed above is developed in this article. Synthesizer drawings the most relevant findings of the ultrastructural pathology, as well as the main metabolic pathways of the PUFAs involved in balance and disbalanced conditions are provided.

show abstract

Effect of dietary docosahexaenoic acid on rhodopsin content and packing in photoreceptor cell membranes

Cited by 34 publications

References 61 publications

Disrupted Blood-Retina Lysophosphatidylcholine Transport Impairs Photoreceptor Health But Not Visual Signal Transduction

Disrupted Blood-Retina Lysophosphatidylcholine Transport Impairs Photoreceptor Health But Not Visual Signal Transduction

Perinatal exposure to diets with different n-6:n-3 fatty acid ratios affects olfactory tissue fatty acid composition

Role of ω3 polyunsaturated fatty acids in diabetic retinopathy: a morphological and metabolically cross talk among blood retina barriers damage, autoimmunity and chronic inflammation

Contact Info

Product

Resources

About