Effect of Desiccation Time on Seed Moisture and Regeneration of Mangaba (Hancornia speciosa) Embryos

Santana, Fernanda Vieira; Oliveira, Annie Carolina Araújo de; Oliveira, Leila Albuquerque Resende de; Miranda, Isabella Cícera Dias; Machado, C. de A.; Santos, Paulo Augusto Almeida; Silva, Ana Veruska Cruz da; Lédo, A. da S.

doi:10.9734/jeai/2018/44644

Cited by 2 publications

(3 citation statements)

References 9 publications

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“…Such behavior suggests that it is necessary to adjust the concentration of the components used in the culture media, and that oxidative stress influenced the obtained regeneration rates. When evaluating the effect of mangabeira desiccation time on moisture and seed regeneration using cryopreserved embryos, Santana et al (2018), did not obtain results with any type of regeneration, which demonstrates that protocols must still be adapted and adjusted according to the cryopreservation technique chosen and the type of explant used.…”

Section: Resultsmentioning

confidence: 96%

“…It is important to note that despite being widely used as an alternative for the conservation of several species, cryopreservation projects must have clear objectives, long-term funding, qualified technical support personnel, the necessary infrastructure, and well-defined protocols so that they can be implemented routinely in plant cryobanks and help establish backup collections of plant genetic resources (Ochatt et al, 2021). The literature reports some cryopreservation protocols in mangabeira, but not all showed satisfactory results (Sartor et al, 2012;Santos et al, 2015;Prudente et al, 2017a;Prudente et al, 2017b;Santana et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effects of droplet vitrification on the regeneration of apical shoot tips of mangabeira accessions

Santana

Oliveira

Santos

et al. 2022

RSD

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The mangabeira (Hancornia speciosa Gomes) is a fruit tree of high potential that is distributed in several regions of the Brazil, but which is of great social economic importance for the Northeast region. Due to the wide possibility of using its fruits that can be consumed in natura or industrialized, and that represent a financial source for traditional communities that practice the extractivism of this species. The objective of this study was to evaluate the efficiency of the droplet vitrification technique for the long-term conservation of five mangabeira accessions, as well as to verify if there is any different behavior among the accessions to the possible effects of the cryopreservation process. The apical shoot tips were subjected to different periods (30 and 50 min for test I; 25, 50 and 75 min for test II) of exposure to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. To evaluate the effects of cryoprotective solutions and exposed times on apical shoot tips exposed or not exposed to liquid nitrogen, the percentage of regeneration were observed at 30th and 60th day of in vitro cultivation. It was possible to cryopreserve the apical shoot tips from five accessions studied through the droplet vitrification technique. Adjusts must be made in the droplet vitrification technique steps in the pre-culture and post-culture media so that there is a mitigation of the damage caused during the cryopreservation.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Effects of droplet vitrification on the regeneration of apical shoot tips of mangabeira accessions

Santana

Oliveira

Santos

et al. 2022

RSD

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“…Cryopreservation strategies, such as storing the embryonic axes of seeds under low temperatures (Ballesteros et al 2014), allow for the conservation of propagules for future in vitro development. Regardless of the advances in cryoprotective techniques and solutions, this strategy still challenges conservation of some species (Santana et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Overcoming Hancornia speciosa seed recalcitrance: harvest season and storage time

Souza

Nunes

Freitas

et al. 2022

Preprint

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Storage of desiccation-tolerant seeds is one of the most effective germplasm conservation strategies; however, several species of tropical and subtropical humid forests have seeds that are sensitive to desiccation, and recalcitrant seeds, making conservation a challenge. Recalcitrant seeds deteriorate during storage due to high respiration rates and metabolic activity, and protocols employing osmoprotective solutions aim to minimize those effects to maintain seed viability for a more extended period. Hancornia speciosa, a fruit tree considered a priority for research in Brazil, is a desiccation-sensitive species. Thus, this study aimed to assess the physiological parameters of viability, vigor, and enzymatic activity of H. speciosa seeds stored in an osmoprotective solution. Germination percentage, water content, electrical conductivity, shoot, root, seedling length, peroxidase activity, and heat-resistant protein concentration were determined for seeds collected during summer and winter harvests. In addition, gene sequences were explored through gene ontology using Blast analysis to identify the biological and molecular processes associated with enzymatic action during storage. Summer-collected seeds performed better in viability and vigor and are recommended for storage in the osmoprotective solution. After being stored in the solution, seeds collected in the winter improved germination and vigor. H. speciosa seeds harvested in the summer or winter and stored in the osmoprotective solution remain viable for up to 90 days. Peroxidase and heat-resistant proteins are active; these enzymes' expression regulation should be investigated in future studies.

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Effect of Desiccation Time on Seed Moisture and Regeneration of Mangaba (Hancornia speciosa) Embryos

Cited by 2 publications

References 9 publications

Effects of droplet vitrification on the regeneration of apical shoot tips of mangabeira accessions

Effects of droplet vitrification on the regeneration of apical shoot tips of mangabeira accessions

Overcoming Hancornia speciosa seed recalcitrance: harvest season and storage time

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