Effect of density gradient material upon ex-vivo neutrophil extracellular trap (NET) formation

Harris, Phillipa; Roberts, Helen; Wright, Helen J.

doi:10.1016/j.freeradbiomed.2012.08.353

Cited by 4 publications

(5 citation statements)

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“…This condition is because neutrophils will start their activities some times after LPS invades cells, and the synthesis of arachidonic acid will begin 4 hours after bacterial exposure. 8,9,14,15 Neutrophil culture cells in this study may experience twice the pressure, namely LPS and the anti-nutrient component (toxic component) of cassava leaf extract. LPS and cassava leaf extract are thought to be extracellular stimuli that cause disruption of COX-2 enzyme production and affect COX-2 expression in cells or tissues.…”

Section: Discussionmentioning

confidence: 90%

“…ROS is a cell selfdefence process against injury for phagocytosis and elimination of pathogenic agents, but excessive production of ROS will attack neutrophil cells themselves and cause cell death. 7,8,11,13 Also, the time of administration of cassava leaf extract which was too short in the study is likely to be the cause of the anti-inflammatory effect of cassava leaf extracts is less optimum. This lack of optimum anti-inflammatory activity is likely correlated with low COX-2 expression in neutrophil cell cultures.…”

Section: Discussionmentioning

confidence: 98%

“…However, excessive neutrophil activity will cause pathological conditions and tissue damage, including periodontal tissue damage. [6][7][8] This neutrophil activity needs to be controlled with anti-inflammatory administration so that it can prevent tissue damage. Nonsteroidal anti-inflammatory drugs (NSAIDs) are the most effective anti-inflammatory drugs to suppress prostaglandin synthesis by inhibiting the production of the COX-2 enzyme.…”

Section: Introductionmentioning

confidence: 99%

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The effect of cassava (Manihot esculenta) leaf extract on COX-2 expression in the neutrophil cell culture exposed to the lipopolysaccharide of Escherichia coli (in-vitro study)

2019

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Introduction: Neutrophils are cells which played an initial role in the inflammation area and can be induced by lipopolysaccharide (LPS). Activated neutrophils will secrete the COX-2 enzyme which is involved in arachidonic acid synthesis to produce inflammatory mediators. However, excessive inflammation can cause tissue destruction; thus, the body needs anti-inflammation agents. Cassava leaf contains biochemical component which has an anti-inflammation effect. This study was aimed to determine the expression of COX-2 in neutrophils administered with cassava leaf extract and the LPS of E. coli. Methods: This study was experimental laboratories (in vitro study) using human neutrophil cells culture. The samples of this study were divided into 4 groups, administered with 12.5% cassava leaf extract (T1); 25% cassava leaf extract (T2); control group which contained only neutrophils cells (C1); and control group contained neutrophils and LPS (C2). Human neutrophil cells culture was incubated in the 12.5% and 25% cassava leaf extract before exposed with the LPS of E. coli. COX-2 expression was observed with immunochemistry methods. Results: The COX-2 expression in the 12.5% cassava leaf extract group was significantly higher than the 25% cassava leaf extract group (p < 0.05). Conclusion: Cassava leaf extract can inhibit the COX-2 expression and can be used as an alternative anti-inflammation agent.Keywords: Anti-inflammation, cassava leaf extract, COX-2 expression, neutrophils.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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The effect of cassava (Manihot esculenta) leaf extract on COX-2 expression in the neutrophil cell culture exposed to the lipopolysaccharide of Escherichia coli (in-vitro study)

2019

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“…The gradient films can be prepared by many methods, which include dissolution and diffusion methods [11], temperature gradient field method [12], as interpenetrating polymer networks [13], by extrusion [14,15], microfluidic techniques [16,17], electrochemical technique [18], centrifugation [19], and so on [20,21,22]. Till date, FGMs have been potentially used in various fields such as medicine [23,24,25,26,27,28], biology [29,30,31,32], and optics [5,33,34,35]. In this regards, preparation of gradient films by incorporating PDMS components along the thickness would be an effective way to fabric the favorable properties of both gelatin and PDMS segments.…”

Section: Introductionmentioning

confidence: 99%

“…However, as a polyelectrolyte, complicated non-covalent interactions exist among gelatin molecules, which are known to be sensitive to a variety of factors including concentration, solvent, pH, temperature and additives such as surfactant [30,31]. In addition, the difference of compatibility between PDMS and gelatin will induce aggregation of PDMS-E grafted gelatin in aqueous solution or organic solvent.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Compositional Gradient Polymeric Films Based on Gradient Mesh Template

Teng

Hou

et al. 2018

Polymers

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In this paper, a template-filling method was found to prepare composition gradient gelatin films by incorporating α-[3-(2,3-epoxypropoxy) propyl]-ω-butyl-polydimethylsiloxane (PDMS–E) grafted gelatin (PGG) into a gradient gelatin mesh template. The method can be used to prepare other composition gradient biopolymer films. Gradient mesh template prepared by the methacrylic anhydride cross-linked gelatin under temperature gradient field. The porosity of the template decreased from 89 to 35% which was accompanied by decrease in average pore size from 160 to 50 µm. Colloidal particles about 0.9~10 µm were formed from PGG after adding them to a mixed solvent system of 9:1 ( v / v ) of ethanol/water, which were filled in the mesh template under vacuum (0.06 MPa). A gradient film was obtained after drying at room temperature for 48 h. The results of scanning electron microscope-energy dispersive X-ray combined with freezing microtome and Fourier transform infrared spectroscopy suggested that the distribution of the Si element along the thickness showed a typical gradient pattern, which led to hydrophilic/hydrophobic continuous changing along the thickness of film. The water vapor permeability, thermal gravimetric analysis, differential scanning calorimetry and dynamic mechanical tensile results show that the gradient films had excellent water vapor permeability and flexibility, and hence could be used as biomimetic materials and leather finishing agents.

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Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

Hirschfeld

Chicca

Moonen

et al. 2022

Methods in Molecular Biology

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Effect of density gradient material upon ex-vivo neutrophil extracellular trap (NET) formation

Cited by 4 publications

References 0 publications

The effect of cassava (Manihot esculenta) leaf extract on COX-2 expression in the neutrophil cell culture exposed to the lipopolysaccharide of Escherichia coli (in-vitro study)

The effect of cassava (Manihot esculenta) leaf extract on COX-2 expression in the neutrophil cell culture exposed to the lipopolysaccharide of Escherichia coli (in-vitro study)

Preparation of Compositional Gradient Polymeric Films Based on Gradient Mesh Template

Characterization, Quantification, and Visualization of Neutrophil Extracellular Traps

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