Effect of cytokines on the generation of active oxygen species by pulmonary and peripheral blood phagocytes

Abstract

The effect of naturally occurring cytokines on the chemiluminescence response of neutrophils from bronchoalveolar lavage and peripheral blood was studied in patients with chronic bronchitis. It was found that the response of neutrophils from bronchoalveolar lavage differs from that of peripheral blood neutrophils. The cytokines prestimulated the peripheral blood neutrophils for chemiluminescence. Key Words: cytokines; chemiluminescence; chronic bronchitisBronchoalveolar neutrophils and macrophages act as effector cells, hnpairments of the phagocytic component of ilnlnunity manifest themselves in reduced activity of alveolar macrophages, decrease in their content in bronchial mucus, increased content of functionally defective neutrophils, high activity of elastase and collagenase, and the presence of active oxygen forms in bronchoalveolar lavage [5,7]. The protective and damaging activities of puhnonary neutrophils have not been studied in sufficient detail. In the present study we used a model of spontaneous and stimulated chemiluminescence to deterlnine the role of cytokines in the regulation of functional activity of puhnonary and peripheral blood neutrophils in chronic nonspecific puhnonary diseases. Functional activity of neutrophils was assessed by measuring luminol-dependent chemiluminescence (LDCL) in an L1251 lmninometer (LKBWallac). Neutrophil suspension was prepared by incubating heparinized (20 U/lnl) venous blood (3 ml) with 3% gelatine solution at 37"C for 15 rain. The resultant cell suspension was twice washed with lnedium 199. Cellular composition was determined by the Zadorozhnyi-Dozlnorov staining. The neutrolghil count was adjusted to 2x106 cells/ml. Bronchoalveolar lavage was obtained by the conventional method. Cell suspension was prepared by filtration of the lavage through a capron filter and centrifugation in medimn 199 15 min at 400g and for 10 min at 200g. The viability and counts of neutrophils and macrophages were deterlnined using Zadorozhnyi-Dozmorov stain. The neutrophil content of the suspension was adjusted to 2x 106 cells/ml. Since the amplitude and the rate of neutrophilic CL response development are greater than those in macrophages [2], we neglected the contribution of lnacrophages to the chemilulninescence of the suspension. The chemiluminescence of other cells (lymphocytes and 0007-4888/98/0010-01035520,00 ~ Kluwer Academic/l)lemm~ |h,bti~h~w~ MATERIALS AND METHODS Neutrophils

Ultraweak Photon Emission as a Non-Invasive Health Assessment: A Systematic Review

“…These studies were grouped by their system of research. The most commonly studied system of research was blood cells (9) [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] followed by whole body (8) [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , blood plasma (4) [39] , [57] , [58] , [59] , tissues (2) [60] , [61] , urine (2) [62] , [63] , saliva (1) [64] , and other cells (1) [65] .…”

Quantifying Biofield Therapy through Biophoton Emission in a Cellular Model

“…When researchers established links among oxidation, free radical production, and an increase in BE, this led to further investigation of using BE as a means to measure an individual’s health ( E. P. Wijk & Wijk, 2005 ; R. Van Wijk, 2001 ). Recent studies have shown an association of increased BE in multiple sclerosis patients ( Hammann et al, 1987 ), ankylosing spondylitis ( K. J. Ho et al, 2000 ), and in chronic lung disease ( Koval’chuk et al, 1998 ). This increase in BE during stress, illness, and disease ( Grasso et al, 1992 ; Keshavarzian et al, 1992 ; E. P. Van Wijk et al, 2010 ) corresponds to a number of studies that show that BE decreases in healthful behavior such as meditation ( E. P. Van Wijk et al, 2006 ; E. P. Van Wijk et al 2008a ; E. P. Van Wijk et al, 2008b ).…”