Effect of cryoprotectants and their concentration on in vitro development of vitrified-warmed immature oocytes in buffalo (Bubalus bubalis)

Wani, Nisar Ahmad; Maurya, S. N.; Misra, A. K.; Saxena, Vijay Laxmi; Lakhchaura, B. D.

doi:10.1016/j.theriogenology.2003.06.002

Cited by 49 publications

(33 citation statements)

References 37 publications

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“…Although there is still considerable debate, published literature tends to confirm the likelihood that these cryoprotectants are not highly toxic. DMSO alone [32] or in combination with other cryoprotectants [33] has been used successfully for oocyte vitrification, and in at least one study [34], the exclusion of DMSO from vitrification solution resulted in lower survival rates of oocytes. However, it has also been reported that DMSO (used alone or in combination with EG) resulted in reduced developmental competence of oocytes [11,18].…”

Section: Discussionmentioning

confidence: 99%

Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development

Prentice-Biensch

Singh

Mapletoft

et al. 2012

Reprod Biol Endocrinol

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BackgroundThe present studies evaluated the effects of cryoprotectants, the vitrification procedure and time in the warming solution containing sucrose on cleavage and embryo development of immature (GV stage) bovine cumulus-oocyte complexes (COCs).MethodsTwo experiments were conducted. In Experiment 1, COCs (n = 420) were randomly assigned to four groups: 1) Control group: no treatment; 2) VS1 group: COCs were exposed to vitrification solution 1 (VS1) containing 7.5% ethylene glycol [EG] + 7.5% dimethyl sulfoxide [DMSO] + 20% calf serum [CS] in TCM-199 at 37 C for 5 min; 3) VS1 + VS2 group: COCs were exposed to VS1 for 5 min followed by VS2 (15% EG + 15% DMSO + 17.1% sucrose + 20% CS) at 37 C for 45–60 sec; and 4) Vitrified group: COCs were exposed to VS1 and VS2, loaded on cryotops, vitrified in liquid nitrogen and then warmed in TCM-199 + 17.1% sucrose + 20% CS at 37 C for 1 min. In Experiment 2, COCs (n = 581) were assigned to the same groups, but those in VS1, VS1 + VS2 and Vitrified groups were sub-divided and exposed to the warming solution for either 1 or 5 min. After treatment and/or warming, all COCs in both experiments underwent in vitro maturation, in vitro fertilization and in vitro culture.ResultsCleavage and blastocyst rates did not differ among Control, VS1 and VS1 + VS2 groups in either experiment. In Experiment 2, there was no effect of time in the warming solution.However, both cleavage and blastocyst rates were lower (P < 0.001) in the Vitrified group than in the Control, VS1 and VS1 + VS2 groups (40.9 and 1.6% vs 92.2 and 34.4%, 79.4 and 25.2%, and 80.2 and 20.8%, respectively in Experiment 1, and 25.0 and 1.7% vs 75.3 and 27.2%, 67.9 and 19.5%, and 62.7 and 22.5%, respectively in Experiment 2).ConclusionsThe permeating cryoprotectants (EG and DMSO) present in VS1 and VS2 solutions and the time in the warming solution containing sucrose had no adverse effects on cleavage and blastocyst rates of immature bovine COCs. However, cleavage rate and early embryo development were reduced following the vitrification and warming.

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Section: Discussionmentioning

confidence: 99%

Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development

Prentice-Biensch

Singh

Mapletoft

et al. 2012

Reprod Biol Endocrinol

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“…In buffalo, limited attempts have been made to cryopreserve immature oocytes by slow freezing (Gautam et al 2008) or vitrification (Dhali et al 2000;Wani et al 2004). To our knowledge, there is no information available on the cryopreservation of mature oocytes by slow freezing, with only one report on the vitrification of mature oocytes (Gasparrini et al 2007) The present study was undertaken to investigate the effects of the type of cryoprotectant and concentration on post-thaw morphology, the nature and extent of injuries and the developmental competence of IVM buffalo oocytes subjected to slow freezing or vitrification.…”

Section: Introductionmentioning

confidence: 99%

Effect of type of cryoprotectant on morphology and developmental competence of in vitro-matured buffalo (Bubalus bubalis) oocytes subjected to slow freezing or vitrification

Gautam

Verma

Palta

et al. 2008

Reprod. Fertil. Dev.

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The present study examined the effects of different cryoprotectants on morphology and developmental competence of in vitro-matured buffalo oocytes after slow freezing or vitrification. After slow freezing in dimethyl sulfoxide (DMSO), ethylene glycol (EG) or 1,2-propanediol (PROH), at 1.0 or 1.5 m each, the proportion of morphologically normal oocytes recovered was significantly higher (P < 0.05) with 1.5 than 1.0 m for all cryoprotectants and was highest (P < 0.05) for 1.5 m DMSO. Following vitrification, the percentage of morphologically normal oocytes recovered was lower (P < 0.01) for 40% EG than for 40% DMSO, 20% EG + 20% DMSO or 20% EG + 20% PROH. The most common damage, irrespective of the cryopreservation method, was loss of cumulus mass. The cleavage rate and the proportion of vitrified-warmed oocytes that developed to morulae/blastocysts were significantly higher (P < 0.01) for 20% EG + 20% DMSO than for the other groups. A higher proportion of oocytes developed to morulae (11.5% v. 4.3%) or blastocysts (5.4% v. 0.6%) after vitrification in 20% EG + 20% DMSO than after slow freezing in 1.5 m DMSO. In conclusion, vitrification was more effective than slow freezing for the cryopreservation of in vitro-matured buffalo oocytes.

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“…These procedures have made it possible to reliably cryopreserve oocytes that are very sensitive to chilling damage and previously have not been cryopreserved successfully. Oocyte vitrification has been reported with various levels of success in the mouse (Stachecki et al 1998a(Stachecki et al , 1998b, human (Kuleshova et al 1999), cattle (Fuku et al 1992;Vajta et al 1998;Hurtt et al 2000;Papis et al 2000), horse (Hurtt et al 2000), goat (Begin et al 2003), pig (Isachenko et al 1998) and buffalo (Dhali et al 2000a(Dhali et al , 2000bWani et al 2004). In buffalo, the first success in production of buffalo blastocysts from vitrified immature oocytes was reported by Wani et al (2004) but blastocyst rates were still low (10-15%).…”

Section: Introductionmentioning

confidence: 99%

Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes

Boonkusol

Faisaikarm

Dinnyés

et al. 2007

Reprod. Fertil. Dev.

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The purpose of the present study was to investigate the effects of two vitrification procedures on developmental capacity and ultrastructural changes of matured swamp buffalo oocytes. In vitro-matured oocytes were vitrified by using 35 and 40% ethylene glycol as vitrification solution for solid surface vitrification (SSV) and in-straw vitrification (ISV), respectively. Survival rate of vitrified-warmed oocytes, evaluated on the basis of ooplasm homogeneity, oolemma integrity and zona pellucida intactness, as well as parthenogenetic blastocyst rates of vitrified-warmed oocytes were significantly higher with SSV (89.3 and 13.6%, respectively) than ISV (81.8 and 5.5%, respectively). However, they were still significantly lower than that of control oocytes (100 and 34.2%, respectively). For examining the ultrastructural changes, fresh, VS-exposed (ISV and SSV), and vitrified-warmed (ISV and SSV) oocytes were processed for transmission electron microscopy. In VS-exposed oocytes, reduction of microvilli abundance and damage of mitochondrial membrane were found only in the ISV group. In vitrified-warmed oocytes, however, it was clear that both methods of vitrification induced profound ultrastructural modifications to microvilli, mitochondria, oolemma and cortical granules as well as to the size and position of vesicles. Damaged mitochondria were, however, more abundant in ISV vitrified oocytes than in SSV vitrified oocytes, which correlated with the developmental data, showing the superiority of the SSV method. The present study demonstrated the feasibility of vitrification of in vitro-matured swamp buffalo oocytes.

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Effect of cryoprotectants and their concentration on in vitro development of vitrified-warmed immature oocytes in buffalo (Bubalus bubalis)

Cited by 49 publications

References 37 publications

Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development

Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development

Effect of type of cryoprotectant on morphology and developmental competence of in vitro-matured buffalo (Bubalus bubalis) oocytes subjected to slow freezing or vitrification

Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes

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