Effect of Constant Glucose Feeding on the Production of Exopolysaccharides by Tremella fuciformis Spores

Abstract

A fed-batch culture system with constant feeding (glucose 80 g L(-1), 0.25 ml min(-1)) was used to study the influence of glucose on cell dry weight and exopolysaccharides production from submerged Tremella fuciformis spores in a 5-L stirred-tank bioreactor. The results showed that high levels of cell mass (9.80 g L(-1)) and exopolysaccharides production (3.12 g L(-1)) in fed-batch fermentation were obtained after 1 h of feeding, where the specific growth rate (mu) and exopolysaccharides yield on substrate consumed (YP/S) were 0.267 d(-1) and 0.14 g g(-1). Unlike batch fermentation, maximal cell mass and exopolysaccharides production merely reached 7.11 and 2.08 g L(-1); the specific growth rate (mu) and exopolysaccharides yield on substrate consumed (YP/S) were 0.194 d(-1) and 0.093 g g(-1), respectively. It is concluded that the synthesis of exopolysaccharides can be promoted effectively when feeding glucose at a late exponential phase.

Lignin-degrading peroxidases in white-rot fungus Trametes hirsuta 072. Absolute expression quantification of full multigene family

“…This data is in agreement with [25,56,57]. So the main source of carbon in the medium (glucose) was almost depleted, and fungus gets under the conditions of carbon starvation and is “searching” for the sources of carbon.…”

Purification of Polysaccharide Produced by the Haploid Yeast Strain of Tremella sanguinea and Its Antioxidant and Prebiotic Activities

“…Fortunately, polysaccharides, the major group of bioactive ingredients in have been extracted and isolated not only from the basidiocarps, but also from mentation culture of the isolated strains [3,6]. Tremella species are heterogene there are two stages in their life cycle, the haploid yeast stage and the filam dikaryon stage; the haploid yeast stage is called tremellomycetous yeast [11][12][13] been found that the basidiospore of both T. fuciformis and T. aurantialba could re by budding and growing as haploid yeast cells [3,[14][15][16]. As it is easier to ac high-density cultivation and shorter production period for yeast, the cultivatio yeast form strain has been considered a more promising alternative for the lar production of polysaccharides from T. fuciformis [14][15][16] and T. aurantialba [3].…”

“…Tremella species are heterogeneous and there are two stages in their life cycle, the haploid yeast stage and the filamentous dikaryon stage; the haploid yeast stage is called tremellomycetous yeast [ 11 , 12 , 13 ]. It has been found that the basidiospore of both T. fuciformis and T. aurantialba could reproduce by budding and growing as haploid yeast cells [ 3 , 14 , 15 , 16 ]. As it is easier to achieve a high-density cultivation and shorter production period for yeast, the cultivation of the yeast form strain has been considered a more promising alternative for the large-scale production of polysaccharides from T. fuciformis [ 14 , 15 , 16 ] and T. aurantialba [ 3 ].…”

“…It has been found that the basidiospore of both T. fuciformis and T. aurantialba could reproduce by budding and growing as haploid yeast cells [ 3 , 14 , 15 , 16 ]. As it is easier to achieve a high-density cultivation and shorter production period for yeast, the cultivation of the yeast form strain has been considered a more promising alternative for the large-scale production of polysaccharides from T. fuciformis [ 14 , 15 , 16 ] and T. aurantialba [ 3 ]. However, polysaccharides produced by the haploid yeast stage of T. sanguinea have not been reported.…”