Effect of Combined Changes in Culture Medium and Incubation Conditions on the Regeneration from Immature Embryos of Elite Varieties of Winter Wheat

Formulation of Nutrient Medium for In Vitro Somatic Embryo Induction in Plantago ovata Forsk

“…Oleifera (Metzg) Sink [20]. Contrary to this result, Tamas et al [54] reported that high-frequency regeneration efficiency was achieved in elite varieties of winter wheat by reducing the concentration of macroelements in the regeneration medium to half strength.…”

Callus induction and plant regeneration by Brazilian new elite wheat genotypes

“…The application of exogenous growth regulators combined with the production of endogenous hormones under genetic and environmental control could influence the hormonal balance, favoring organogenesis or embryogenesis, thus, allowing specific genotypes responses in determined media (Jiménez andBangerth 2001, Li et al 2003). In relation to wheat, Tamás et al (2004) showed that the endogenous hormone balance influences the regeneration of green spots as well as the appearance of plantlets. The authors detected an interaction between genotype, type of explant, composition of the culture medium and cultivation conditions determining the genotype regeneration capacity.…”

“…Several factors can influence the regenerative potential of a specimen: genotype, concentration of different growth regulators, explant types and sizes, culture media and culture conditions (El-Sherbeny et al 2001, Tamás et al 2004. Several media for tissue culture used with immature embryos are quoted in literature: MS (Musharige and Skoog 1962) (Harvey et al 1999, Gonzalez et al 2001); N6 (Wu et al 2002); B5 (Vasil and Vasil 1994) and modified MS -MB (Li et al 2003); P (Delporte et al 2001); CM4/MMS (Cheng et al 1997); and DSEM (Eudes et al 2003), with different combinations of components and growth regulators.…”

Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.)

“…On the other hand, establishment of an efficient regeneration system is necessary for the genetic engineering of wheat. The explants source, genotype and medium composition were shown to influence the frequency of callus induction and regeneration in the wheat tissue culture [8][9][10][11]. In particular, genotype and explants type are important factors for embryogenic callus production and regeneration [12].…”